Human periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitro

Hasegawa, Tomoka; Kikuiri, Takashi; Takeyama, Sadaaki; Yoshimura, Yoshitaka; Mitome, Masato; Oguchi, Haruhisa; Shirakawa, Tetsuo

doi:10.1054/tice.2002.0223

Cited by 41 publications

(36 citation statements)

References 31 publications

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“…And TRAP ( + ) osteoclast-like cells could be induced when DPDLCs were cocultured with mouse bone marrow cells [7]. However, as the stem cells derived from PDL and as the progenitors for (E-F) Western blot assay showed expression of Runx2, RANKL, and OPG after knockdown of Runx2.…”

Section: Discussionmentioning

confidence: 98%

“…Previous studies indicated that periodontal ligament (PDL) tissue played an important role in regulating osteoclast differentiation, suggesting PDL might be involved in root resorption [7,8]. Periodontal ligament stem cells (PDLSCs), which were isolated from periodontal tissue, are capable of differentiating into osteoblast, adipocyte, and tooth cementoblast in vitro and regenerating cementum/PDL tissue in vivo [9].…”

mentioning

confidence: 99%

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Periodontal Ligament Stem Cells Modulate Root Resorption of Human Primary Teeth via Runx2 Regulating RANKL/OPG System

Wang

Dong

et al. 2014

Stem Cells and Development

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Physiological primary teeth exfoliation is a normal phenomenon during teeth development. However, retained primary teeth can often be observed in the patients with cleidocranial dysplasia (CCD) caused by mutation of Runx2. The potential regulative mechanism is still unknown. In the present study, periodontal ligament stem cells (PDLSCs) were derived from different resorbed stages of primary teeth and permanent teeth from normal patients and primary teeth from CCD patient. The proliferative, osteogenic and osteoclast-inductive capacities of PDLSCs from each group were detected. We demonstrated here that the proliferative ability of PDLSCs was reduced while the osteogenic and the osteoclast-inductive capacity of PDLSCs were enhanced during root resorption. The results also showed that PDLSCs from permanent teeth and CCD patient expressed low level of Runx2 and RANKL while high level of OPG. However, expression of Runx2 and RANKL were increased while expression of OPG was decreased in PDLSCs derived from resorbed teeth. Furthermore, Runx2 regulating the expression of RANKL and OPG and the osteoclast-inductive capacity of PDLSCs were confirmed by gain or loss of function assay. These data suggest that PDLSCs promote osteoclast differentiation via Runx2 upregulating RANKL and downregulating OPG, leading to enhanced root resorption that results in physiological exfoliation of primary teeth.

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

Periodontal Ligament Stem Cells Modulate Root Resorption of Human Primary Teeth via Runx2 Regulating RANKL/OPG System

Wang

Dong

et al. 2014

Stem Cells and Development

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“…Hasegawa et al 8 reported that PDL cells derived from deciduous and permanent teeth synthesized both RANKL and OPG, and could regulate the differentiation of osteoclasts. It was also reported that PDL cells secrete M-CSF in response to TNF-α stimulation.…”

Section: Figmentioning

confidence: 99%

Effects of compressive stress on the expression of M-CSF, IL-1β, RANKL and OPG mRNA in periodontal ligament cells

et al. 2009

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Objective: The aim of this study was to determine if human PDL cells can produce osteoclastogenic mRNA and examine how compressive stress affects the expression of osteoclastogenic mRNA in human PDL cells. Methods: Human PDL cells were obtained from biscupids extracted for orthodontic treatment. The compressive force was adjusted by increasing the number of cover glasses. PDL cells were subjected to a compressive force of 0.

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“…The mechanisms of molecular control of odontoclasts has not been fully explained. However, it has been shown that the RANK receptor is expressed by odontoclasts, and RANKL, by odontoblasts, pulp and periodontal ligament [37][38][39].…”

Section: Clinical Manifestations In the Oral Cavity In Patients With mentioning

confidence: 99%

Clinical immunology Clinical manifestations in the oral cavity in patients with hyper-IgE syndrome.

Olczak‐Kowalczyk¹,

Matosek²,

Heropolitańska-Pliszka³

et al. 2013

cejoi

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Human periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitro

Cited by 41 publications

References 31 publications

Periodontal Ligament Stem Cells Modulate Root Resorption of Human Primary Teeth via Runx2 Regulating RANKL/OPG System

Periodontal Ligament Stem Cells Modulate Root Resorption of Human Primary Teeth via Runx2 Regulating RANKL/OPG System

Effects of compressive stress on the expression of M-CSF, IL-1β, RANKL and OPG mRNA in periodontal ligament cells

Clinical immunology Clinical manifestations in the oral cavity in patients with hyper-IgE syndrome.

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