Human periodontal ligament cell sheets cultured on amniotic membrane substrate

Adachi, Kôichi; Amemiya, Takeshi; Nakamura, Takahiro; Honjyo, K.; Kumamoto, Shoichiro; Yamamoto, Toshiro; Bentley, Adam J.; Fullwood, Nigel J.; Kinoshita, Shigeru; Kanamura, Narisato

doi:10.1111/odi.12176

Cited by 17 publications

(24 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oral mucosal epithelial cell [78] Corneal epithelium [80] Not needed Amniotic membrane Oral mucosal epithelial cell [7], Corneal endothelial cell [8], Corneal epithelium [10] Conjunctival epithelial cell [9], Amniotic epithelial cell [81], Keratinocyte [82], PDL cell [83] Temperature Temperature-responsive Somatic cell (epithelial): Corneal epithelium [51], reduction below polymer (PIPAAm)-Keratinocyte [13], Corneal epithelial cell [14], Esophageal mucosa 32°C grafted cell culture vessel Oral mucosal epithelial cell [15], RPE cell [16], [52] Middle ear mucosal cell [17], Urothelial cell [18], Alveolar epithelial cell [19], Tracheal epithelial cell [20] Somatic cell (non-epithelial):…”

Section: Key Materials Cell Type (Pre-clinical Study) Target Tissue Stmentioning

confidence: 99%

Cell sheet engineering for regenerative medicine: Current challenges and strategies

Owaki

Shimizu

Yamato

et al. 2014

Biotechnology Journal

161

135

View full text Add to dashboard Cite

Substantial progress made in the areas of stem cell research and regenerative medicine has provided a number of innovative methods to repair or regenerate defective tissues and organs. Although previous studies regarding regenerative medicine, especially those involving induced pluripotent stem cells, have been actively promoted in the past decade, there remain some challenges that need to be addressed in order to enable clinical applications. Designed for use in clinical applications, cell sheet engineering has been developed as a unique, scaffold-free method of cell processing utilizing temperature-responsive cell culture vessels. Clinical studies using cell sheets have shown positive outcomes and will be translated into clinical practice in the near future. However, several challenges stand in the way of the industrialization of cell sheet products and the widespread acceptance of regenerative medicine based on cell sheet engineering. This review describes current strategies geared towards the realization of the regenerative medicine approach.

show abstract

Section: Key Materials Cell Type (Pre-clinical Study) Target Tissue Stmentioning

confidence: 99%

Cell sheet engineering for regenerative medicine: Current challenges and strategies

Owaki

Shimizu

Yamato

et al. 2014

Biotechnology Journal

161

135

View full text Add to dashboard Cite

show abstract

“…More importantly, in addition to the ability to differentiate into osteoblast-like cells and adipocytes, PDLSCs can also specifically differentiate into periodontal cells, such as cementoblast-like cells and collagen forming cells in vitro, and they possess the capacity to generate a cementum/PDL-like structure when they were transplanted into nude mouse and preclinical periodontal defect models. [5][6][7][8][9][10][11][12] Conventional hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 , HA) bioceramics had been used in periodontal bone tissue repair for their chemical similarity to bone mineral and their excellent biological activity and osteoconductivity. 13 However, it is reported that HA bioceramics lack osteoinductive ability, and consequently possess limited ability to form an interface with new bone tissue and hence hamper the new bone formation.…”

Section: Introductionmentioning

confidence: 99%

Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

Mao¹,

Liu²,

Zhao³

et al. 2015

IJN

View full text Add to dashboard Cite

The surface structure of bioceramic scaffolds is crucial for its bioactivity and osteoinductive ability, and in recent years, human periodontal ligament stem cells have been certified to possess high osteogenic and cementogenic differential ability. In the present study, hydroxyapatite (HA) bioceramics with micro-nano-hybrid surface (mnHA [the hybrid of nanorods and microrods]) were fabricated via hydrothermal reaction of the α-tricalcium phosphate granules as precursors in aqueous solution, and the effects of mnHA on the attachment, proliferation, osteogenic and cementogenic differentiations of human periodontal ligament stem cells as well as the related mechanisms were systematically investigated. The results showed that mnHA bioceramics could promote cell adhesion, proliferation, alkaline phosphatase (ALP) activity, and expression of osteogenic/cementogenic-related markers including runt-related transcription factor 2 (Runx2), ALP, osteocalcin (OCN), cementum attachment protein (CAP), and cementum protein (CEMP) as compared to the HA bioceramics with flat and dense surface. Moreover, mnHA bioceramics stimulated gene expression of low-density lipoprotein receptor-related protein 5 ( LRP5 ) and β-catenin , which are the key genes of canonical Wnt signaling. Moreover, the stimulatory effect on ALP activity and osteogenic and cementogenic gene expression, including that of ALP, OCN, CAP, CEMP, and Runx2 of mnHA bioceramics could be repressed by canonical Wnt signaling inhibitor dickkopf1 (Dkk1). The results suggested that the HA bioceramics with mnHA could act as promising grafts for periodontal tissue regeneration.

show abstract

“…This was evident as the filaments on the basement side were more compact than those on the stromal side of the HAM. Adachi et al (2014) demonstrated that the stromal surface showed a hemi-desmosomal attachment between the cellmembrane interface, while only physical interlocking was seen on the basement membrane side of the HAM, as evident from both the transverse electron microscopy and immunohistochemistry.…”

Section: Discussionmentioning

confidence: 85%

“…Over the past few decades, with advancements in tissue engineering, biomaterial science and scaffold design, this 'miracle' membrane has been introduced into periodontal tissue engineering (Litwiniuk & Grzela 2014). Adachi et al (2014) demonstrated that periodontal cells cultured on amniotic membrane substrates express essential proteins for cell-substrate adhesion and the maintenance of tissue integrity. The HAM has also been shown to have good adhesion with periosteal-derived cell sheets and dental pulp-derived cell sheets Honjo et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Human Amniotic Membrane as a Scaffold for Periodontal Ligament Fibroblast Attachment and Proliferation

Elahi¹,

Taib²,

Berahim³

et al. 2019

JSM

View full text Add to dashboard Cite

This study was aimed at evaluating the ability of the human amniotic membrane (HAM) to act as a scaffold for the growth of the main cells in periodontal regeneration, human periodontal ligament fibroblasts (HPDLFs). The HAM has many biological properties that are suitable for periodontal tissue regeneration such as low immunogenicity, anti-fibrosis, anti-inflammation, and a rich extracellular matrix component. Commercially available HPDLFs were seeded onto the HAM, and the attachment and proliferation of the cells were observed through scanning electron microscopy (SEM) and histological analysis. Cell viability was assessed using the alamarBlue® proliferation assay at days 1, 3, 7, 14 and 21. Histologically, the HPDLFs showed a monolayer to multilayer attachment onto the HAM from day 1 to day 7. The SEM analysis demonstrated that the HPDLFs had attached appropriately onto the HAM surface at day 1 to day 3, and began overlapping at day 7, while maintaining their flat shape. However, by days 14 and 21, there was an alteration in the morphology of the cells, where they later became rounded. The proliferation assay showed that the viability of the HPDLFs on the HAM had increased significantly from day 1 to day 7 (p=0.012), but later showed significant reduction at day 14 (p=0.002) and day 21 (p=0.005). In conclusion, this study showed that the HAM was able to function well as a scaffold for HPDLFs within 7 days, and thus, it can be a promising scaffold for periodontal regeneration. However, the behaviour of the cells in relation to the membrane over longer culture duration warrants further investigation. ABSTRAK Kajian ini bertujuan untuk menilai keupayaan membran amniotik manusia (HAM) untuk bertindak sebagai perancah bagi pertumbuhan sel-sel utama dalam regenerasi periodontal iaitu sel fibroblas ligamen periodontal manusia (HPDLFs). HAM mempunyai banyak ciri biologi yang sesuai untuk pertumbuhan semula tisu periodontal seperti keimunogenan yang rendah, anti-fibrosis, anti-keradangan dan kaya dengan komponen matriks luar sel. HPDLFs yang tersedia secara komersial telah dibiakkan ke atas HAM, dan pelekatan serta pengembangbiakan HPDLFs diperhatikan melalui pemeriksaan mikroskop imbasan elektron (SEM) dan analisis histologi. Daya tahan sel telah dinilai menggunakan ujian percambahan alamarBlue® pada hari 1, 3, 7, 14 dan 21. Secara histologi, HPDLFs menunjukkan pelekatan ekalapisan kepada beberapa lapisan ke atas HAM daripada hari pertama hingga ke-7. Analisis SEM menunjukkan bahawa HPDLFs telah melekat dengan baik pada permukaan HAM pada hari pertama hingga hari ke-3 dan mula bertindih pada hari ke-7, sambil mengekalkan bentuknya yang rata. Bagaimanapun, pada hari ke-14 dan ke-21, terdapat perubahan pada morfologi sel, dengan mereka kemudiannya menjadi bulat. Ujian percambahan menunjukkan bahawa daya tahan sel HPDLFs pada HAM meningkat dengan ketara daripada hari pertama hingga hari ke-7 (p=0.012) namun kemudiannya menunjukkan penurunan yang ketara pada hari ke-14 (p=0.002) dan hari ke-21 (p=0.005). Kesimpulannya, kaj...

show abstract

Human periodontal ligament cell sheets cultured on amniotic membrane substrate

Abstract: We conclude that AM may represent a suitable substrate for culturing PDL cells and that PDL cells cultured on AM show sheet formation.

Cited by 17 publications

References 36 publications

Cell sheet engineering for regenerative medicine: Current challenges and strategies

Cell sheet engineering for regenerative medicine: Current challenges and strategies

Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

Evaluation of the Human Amniotic Membrane as a Scaffold for Periodontal Ligament Fibroblast Attachment and Proliferation

Contact Info

Product

Resources

About