Human parathyroid antigen: characterization and localization with monoclonal antibodies.

Cance, William G.; Wells, Samuel A.; Dilley, William G.; Welch, Michael J.; Otsuka, Fyllis L.; Davie, Joseph M.

doi:10.1073/pnas.83.16.6112

Cited by 10 publications

(5 citation statements)

References 28 publications

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“…From this study and the reports of Posillico's group [1] and our group [2], however, it can be concluded that the parathyroid cell has a variety of surface molecules, some of which are shared by other endocrine tissues, others of which appear unique to the parathyroid cell, and others of which are restricted to the parathyroid and kidney. For many of these reported markers, specific monoclonal antibody binding can affect parathyroid hormone release and/or cellular calcium changes.…”

supporting

confidence: 39%

Invited commentary

Cance

1988

World j. surg.

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supporting

confidence: 39%

Invited commentary

Cance

1988

World j. surg.

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“…At present, we do not know whether the monoclonal antiparathyroid antibody recently reported by Cance et al [18] recognizes similar structures as the presently used antibodies. The former antibody appears to react exclusively with parathyroid cells and its functional effects has not been reported on.…”

Section: Discussionmentioning

confidence: 88%

Monoclonal antiparathyroid antibodies revealing defect expression of a calcium receptor mechanism in hyperparathyroidism

et al. 1988

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Monoclonal antibodies were generated with a hybridoma technique after immunization of mice with intact human parathyroid cells. Three antibodies of the IgG type reacted in immunohistochemistry and immunofluorescense with parathyroid epithelial cells and proximal tubule cells of the kidney but not with a large number of other human tissues. Adenomatous and hyperplastic parathyroid glands of patients with hyperparathyroidism (HPT) demonstrated a reduced immunohistochemical reactivity with the antibodies as compared to the intense staining of normal human parathyroid tissue. Experiments with dispersed parathyroid cells from the normal and pathological glands showed that 2 of the antibodies blocked the effects of ambient calcium on cytoplasmic calcium and parathyroid hormone release. This indicates that the antibodies interfere with a newly recognized receptor mechanism of parathyroid cells involved in the sensing and gating of calcium and thereby also in the regulation of parathyroid hormone release. A reduced expression of the putative calcium receptor mechanism of abnormal parathyroid cells may, thus, be principally responsible for the hypercalcemia of HPT by causing a decreased sensitivity to extracellular calcium of the parathyroid hormone release from pathological parathyroid glands. The antibodies should improve the parathyroid histological diagnosis by their ability to identify and to distinguish between normal and abnormal parathyroid tissue.

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“…Previous authors have described successful intramuscular parathyroid tissue implantation through a skin incision 2,8,9 . The technique of implantation used in the present study differed in that the tissue was injected intramuscularly into the mouse's flank.…”

Section: Methods and Resultsmentioning

confidence: 99%

“…Previous authors have described successful intramuscular parathyroid tissue implantation through a skin incision. 2,8,9 The technique of implantation used in the present study differed in that the tissue was injected intramuscularly into the mouse's flank. To do this, a 1 3 l-mm to 1 3 2-mm fragment of tissue was drawn up into the hub of a 1Cgauge needle and was injected into the muscle.…”

Section: Experiments 1: Mansplantation Of Human Parathyroid Tissuementioning

confidence: 99%

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Parathyroid Visualization With Cibacron Blue/BB5‐G1

1996

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BB5-G1 is a murine monoclonal antibody with proven affinity for human parathyroid tissue. The authors conjugated BB5-G1 to cibacron blue, a water-soluble dye, in an attempt to enhance intraoperative parathyroid visualization. Athymic nude mice possessing intramuscularly implanted human parathyroid tissue were infused with the conjugate, then surgically explored. Intraoperative findings were video-captured, then analyzed by three independent observers and by computer, generating subjective and objective data quantifying parathyroid visualization. Parathyroid tissue implants were stained and were easily visualized. The implants in the test mice displayed higher computer-generated color intensity ratings (mean, 10.25 units) compared to control mice (mean, 6.10 units) (p<0.5). Histologic sections confirmed the presence of dye and antibody within parathyroid tissue. This represents the first study to use such a conjugate to selectively enhance parathyroid visualization.

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Human parathyroid antigen: characterization and localization with monoclonal antibodies.

Cited by 10 publications

References 28 publications

Invited commentary

Invited commentary

Monoclonal antiparathyroid antibodies revealing defect expression of a calcium receptor mechanism in hyperparathyroidism

Parathyroid Visualization With Cibacron Blue/BB5‐G1

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