Human papillomavirus viral load as a useful triage tool for non-16/18 high-risk human papillomavirus positive women: A prospective screening cohort study

Li, Dong; Wang, Margaret; Zhao, Xinghong; Feng, Rui; Hu, Shang Ying; Zhang, Qian; Smith, Jennifer S.; Qiao, You‐Lin; Zhao, Fen

doi:10.1016/j.ygyno.2017.11.016

Cited by 28 publications

(42 citation statements)

References 47 publications

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“…Several events may modulate E6 and E7 transcript rates in HPV positive tumors. As mentioned above, variable HPV viral loads may be observed in oropharyngeal squamous cell carcinomas and this could modify HPV-induced carcinogenesis as described in cervical cancer [30,31]. Some epigenetic events could enhance E6 or E7 transcription, such as a hypomethylation of p97 promoter at the E6 proximal part of the non-coding locus control region present in the HPV genome [32].…”

Section: Discussionmentioning

confidence: 97%

“…RNA CISH assay was performed to detect high risk HPV E6 and E7 transcripts (RNAscope 2.0 BROWN assay kit), using the HPV-HR18 probe cocktail (Advanced Cell Diagnostics Inc.), according to the manufacturer's instructions. This cocktail contains probes targeting E6 and E7 transcripts from 18 high risk HPV genotypes (HPV16, 18,26,31,33,35,39,45,51,52,53,56,58,59, 66, 68, 73, and 82). A case was considered positive in the presence of a brown punctiform staining in the cytoplasm of tumor cells.…”

Section: Pathology and Immunohistochemistrymentioning

confidence: 99%

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HPV RNA CISH score identifies two prognostic groups in a p16 positive oropharyngeal squamous cell carcinoma population

et al. 2018

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HPV-related and HPV-unrelated oropharyngeal squamous cell carcinomas are two distinct entities according to the Union for International Cancer Control, with a better prognosis conferred to HPV-related oropharyngeal squamous cell carcinomas. However, variable clinical outcomes are observed among patients with p16 positive oropharyngeal squamous cell carcinoma, which is a surrogate marker of HPV infection. We aimed to investigate the prognostic value of RNA CISH against E6 and E7 transcripts (HPV RNA CISH) to predict such variability. We retrospectively included 50 histologically confirmed p16 positive oropharyngeal squamous cell carcinomas (p16 positive immunostaining was defined by a strong staining in 70% or more of tumor cells). HPV RNA CISH staining was assessed semi-quantitatively to define two scores: RNA CISH "low" and RNA CISH "high". Negative HPV RNA CISH cases were scored as RNA CISH "low". This series contained 29 RNA CISH low cases (58%) and 21 RNA CISH high cases (42%). Clinical and pathologic baseline characteristics were similar between the two groups. RNA CISH high staining was associated with a better overall survival in both univariate and multivariate analyses (p = 0.033 and p = 0.042, respectively). Other recorded parameters had no prognostic value. In conclusion, HPV RNA CISH might be an independent prognostic marker in p16 positive oropharyngeal squamous cell carcinomas and might help guide therapeutics.

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Section: Discussionmentioning

confidence: 97%

Section: Pathology and Immunohistochemistrymentioning

confidence: 99%

HPV RNA CISH score identifies two prognostic groups in a p16 positive oropharyngeal squamous cell carcinoma population

et al. 2018

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“…High viral load has been suggested as a marker of non-transient infection and it has an elevated likelihood of having cells with transforming infections and viral integration into the cellular genome as increasing number of infected cells and/or increasing numbers of viral copies occur in individual cells [5,17]. However, association between HPV viral load and cervical lesion grade is controversial [6,8]. There could be undetermined confounding factors [12,14].…”

Section: Discussionmentioning

confidence: 99%

“…HPV genotyping by HybriMax was performed using an HPV GenoArray Test Kit (HybriBio Ltd., China). This assay can determine 21 HPV types, including 14 high-risk HPV types (16,18,31,33,35,39,45,51,52,56,58,59, 66, and 68), five low-risk HPV types (6,11,42,43, and 44), and two unknown-risk types (53 and CP8304), by the flow-through hybridization technique using HPV DNA amplified by PCR as described before [16].…”

Section: Detection Of Hpv-dnamentioning

confidence: 99%

“…However, the relation between HR-HPV viral load and future risk of HSIL or CC is less certain. Many studies have reported that viral load increases with the elevation of disease severity [6,7], while others have not confirmed such an increased risk of disease progression [8,9]. Integration of the viral genome is a prerequisite for establishing a causal role in the carcinogenic process [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Analysis on influencing factors of viral load in patients with high-risk human papillomavirus

Wang

Zhang

et al. 2020

Preprint

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Background High-risk human papillomavirus (HR-HPV) load is thought to be influenced by many factors, and relationship between viral load and degree of cervical lesion is controversial. This study was to explore possible influencing factors of HR-HPV viral load in uterine cervix. Methods 605 women who needed colposcopic evaluation for abnormal cervical screening in Affiliated Hospital of Weifang Medical University, China, between November 2017 and September 2018 were enrolled. Cervical specimens were collected from endo- and ectocervix separately using two different cervical brushes. Hybrid Capture II test was used to measure HR-HPV load. Age, histological severity, number of viral types, area and location of cervical lesions were recorded. The correlation between viral load and influencing factors was analysed using univariate and multivariate analysis. Results HR-HPV load was positively correlated with age, histological severity, multiple HPV types and area of cervical lesions (P < 0.05). Viral load with combination of endo- and ectocervical sampling was significantly higher than simple endocervical sampling (P < 0.001). Multivariate analysis showed that age, multiple HPV types and area of cervical lesions were independent factors for HR-HPV load with combination of endo- and ectocervical sampling (P < 0.05). However, only age and area of cervical lesions were independent factors for viral load with simple endocervical sampling (P < 0.05). No significant association was found between viral load and lesion severity in multivariate analysis (P > 0.05). Conclusion HR-HPV load is influenced by age, histological severity, multiple viral types, area of cervical lesion and sampling methods. Age and area of cervical lesions are independent factors for viral load.

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Diagnostic value of high‐risk HPV other than type 16/18 in high‐grade cervical neoplasia among cytology‐negative women: A multicenter retrospective study

Bai

Xue

et al. 2023

Cancer Medicine

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Background: Human papillomavirus (HPV) is a necessary cause of cervical cancer, and a tool more sensitive than cytology for the early screening of cervical precancers. The two most carcinogenic genotypes HPV 16/18 have been reported in the majority of studies. High-risk HPVs other than HPV 16/18 (non-16/18-hrHPVs) cause approximately a quarter of cervical cancers, and we aimed to analyze the genotype-specific prevalence, risk and diagnostic efficiency of non-16/18-hrHPVs in cervical carcinogenesis among Chinese cytology-negative women.Methods: A total of 7043 females who had abnormal cervical testing results from January 2018 to October 2021 were enrolled, among them 3091 were cytologynegative. Descriptive statistics was used to estimate the HPV genotype-specific prevalence, and multivariable logistic regression was used to estimate the genotype-specific non-16/18 hrHPVs risk of cervical carcinogenesis. The evaluation of diagnostic value among HPV genotypes included the possibility of predicting cervical intraepithelial neoplasia grade 2/3 or worse (CIN2+/CIN3+) and the diagnostic efficiency measured by increased referral rate and referral numbers of colposcopies per additional CIN2+/CIN3+ detected. Results: Among HPV-positive cytology-negative women, the five dominant genotypes for CIN2+/CIN3+ were HPV 31/33/35/52/58. HPV 52/58/33 had comparatively high sensitivity and specificity in predicting CIN2+/CIN3+, while the referral strategy of multiple HPV58 required 26 colposcopies to detect 1 CIN3+, compared with 14, 12, and 8 required by multiple HPV52, 31, and 33, respectively.Conclusions: HPV31/33/35/52/58 infections are significant risk factors for cervical lesions, and multiple HPV 31/33/52 infections should be included in the previously recommended HPV16/18 genotyping triage for colposcopy in China, as the benefits of disease prevention may outweigh the disadvantages of increasing requirements for colposcopy services.

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Human papillomavirus viral load as a useful triage tool for non-16/18 high-risk human papillomavirus positive women: A prospective screening cohort study

Abstract: Our findings support the guidelines in referring all HPV16/18 positive women to colposcopy and suggest triaging non-16/18 hrHPV positive women using viral loads in resource-limited areas where cytology screening was inaccessible.

Cited by 28 publications

References 47 publications

HPV RNA CISH score identifies two prognostic groups in a p16 positive oropharyngeal squamous cell carcinoma population

HPV RNA CISH score identifies two prognostic groups in a p16 positive oropharyngeal squamous cell carcinoma population

Analysis on influencing factors of viral load in patients with high-risk human papillomavirus

Diagnostic value of high‐risk HPV other than type 16/18 in high‐grade cervical neoplasia among cytology‐negative women: A multicenter retrospective study

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