Human papillomavirus typing of invasive cervical cancers in Italy

Abstract

Background: Human papilloma viruses (HPV) are the necessary cause of invasive cervical cancer (ICC). Of the many different types identified so far, only a few of them account for the great majority of cases worldwide, with geographical differences in their distribution. Data on the local distribution are now of interest in view of the soon-to-come introduction of HPV type-specific prophylactic vaccines.

HPV-16 E6 L83V variant in squamous cell carcinomas of the upper aerodigestive tract

“…Sequence analysis of the E6 gene of our HPV16-positive UADT-SCCs showed the presence of the T350G mutation in 5/8 cases, all of which located in the oral cavity and oropharynx [5/6 (83.33%)], while the two cases in the larynx and hypopharynx contained HPV 16 prototype sequences. Of the naturally occurring HPV 16 viral variants, the T350G (L83V) is the most frequently found among invasive cervical cancers (Del Mistro et al 2006;Tornesello et al 2004) and has been linked to a higher oncogenic potential than the prototype (Lichtig et al 2006). The E6 variant distribution found among our UADT cases (5/8) reXects the frequency observed in this area by sequence analysis in cervical samples; the L83V variant predominates over the prototype, with a higher prevalence among women with invasive cervical cancer and high grade lesions than among women with no or low grade lesions (Tornesello et al 2004, data not shown).…”

“…HPV types were identiWed by restriction fragment length polymorphism (RFLP) analysis of the ampliWed products. AmpliWcation of a 323-bp fragment in the HPV 16 E6 region by type-speciWc primers H16L1/H16R3 was also performed on all samples (Del Mistro et al 2006); type-speciWc primers 16E2-1/16E2-2 that amplify a 1,192-bp fragment in the E2 region were used on HPV 16 positive samples (Das et al 1992). DNA from SiHa (HPV 16 positive) and HeLa (HPV 18 positive) cell lines were used as positive controls.…”

Comparison of a novel HPV test with the Hybrid Capture II (hcII) and a reference PCR method shows high specificity and positive predictive value for 13 high-risk human papillomavirus infections

“…Certain types of HPV (high-risk types) have been found to be closely associated with the development of greater than 90% of cervical cancers (Chacón et al, 2007;Cuschieri et al, 2004;Del Mistro et al, 2006). The use of combined tests to detect the presence of high-risk HPV DNA together with Pap testing has been shown to greatly improve the ability to detect pre-cancerous states (Wright et al, 2004).…”

Extensive anal condylomatosis: prognosis in relation to viral and host factors

“…Most of the data on HPV 16 viral variants have been obtained from cervical lesions. The T350G (L83V) isolate is the most commonly found among invasive carcinoma [14,24] and has been linked to an increased risk of progression of cervical disease [25]. The number of our cases is too small to draw any conclusion, but it is noteworthy that two of three patients harbouring the L83V variant developed invasive carcinoma, and that the only death occurred in one of these patients.…”