Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix

Pater, Mary M.; Pater, Alan

doi:10.1016/0042-6822(85)90164-3

Cited by 256 publications

(161 citation statements)

References 13 publications

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“…Previously, cells of myeloid origin such as dendritic cells were the only cell types to express TREM-2 that were known to interact with gonococci (48,49). For the immortalized cell lines, however, it should be noted that they contain DNA sequences from human papillomaviruses which could theoretically influence TREM-2 expression (50,51), and as such they may not accurately represent the expression of TREM-2 on primary reproductive tract epithelial cells lacking papillomavirus DNA.…”

Section: Discussionmentioning

confidence: 99%

TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells

et al. 2008

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Triggering receptor expressed on myeloid cells-2 (TREM-2) is an innate immune receptor that initiates cellular activation upon ligation. In this study, we examined the interaction of TREM-2 with Neisseria gonorrhoeae using murine TREM-2A as it has been reported to recognize bacterial ligands. Using a whole bacteria ELISA, TREM-2A bound to all 6 strains in variable degrees. Far western blots of gonococcal outer membranes revealed TREM-2A binding to lipooligosaccharide (LOS) and Opa protein with predominant binding to LOS. Binding of TREM-2A to LOS was confirmed by ELISA and surface plasmon resonance. O-deacylation of the lipid A significantly reduced binding. Flow cytometry and reporter cell assays showed that gonococci bound to TREM-2A-transfected cells and induced transmembrane signaling. In humans, TREM-2 was constitutively expressed by genitourinary and fallopian tube epithelial cells, both of which are primary targets of gonococcal invasion. Ligation of TREM-2 by LOS induced IL-6 production in HeLa cervical carcinoma cells. To our knowledge, this is the first report of the expression of human TREM-2 by cells deriving from a non-myeloid lineage. We conclude that gonococci can interact with TREM-2 receptors through binding to LOS and Opa protein and initiate cell signaling and cytokine production.

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Section: Discussionmentioning

confidence: 99%

TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells

et al. 2008

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“…Malignant lesions frequently contain HPV DNA integrated into the cellular genome with only a portion of the viral DNA present (Durst et al, 1985). Invariably, two viral genes, E6 and E7, are intact and expressed (Matsukura et al, 1986;Pater & Pater, 1985;Seedorf et al, 1987). It is well established that HPV-16 and HPV-18 can immortalize primary human cells, such as keratinocytes (Durst et al, 1987b;Kaur & McDougall, 1988;Woodworth et al, 1989) and fibroblasts (Pirisi et al, 1987;Watanabe et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Characterization of primary human fibroblasts transformed by human papilloma virus type 16 and herpes simplex virus type 2 DNA sequences

Dhanwada

Veerisetty

Zhu

et al. 1992

Journal of General Virology

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Human papilloma virus type 16 (HPV-16) and herpes simplex virus type 2 (HSV-2) are human viruses implicated in the development of cancer, in particular cervical cancer. The ability of HSV-2 and HPV-16 to transform early passage human cells was examined in this report. For these studies, gingival fibroblasts were utilized. One gingival cell strain was derived from a normal individual (N-16). The second cell strain was derived from hyperplastic gingival tissue of an epileptic individual (R-30) treated with phenytoin, an antiseizure drug. A common side effect of phenytoin is the induction of gingival overgrowth. R-30 cells contained a stable chromosomal translocation between chromo-

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“…Ca Ski, HeLa and C-33A cell lines were studied due to the differential response to cisplatin, Table 1, based on their HPV status [40]. Ca Ski cell lines contain evidence of HPV-16 as well HPV-18 sequences [41], HeLa cell lines contain HPV-18 sequences [41]. The C-33A cell line is a HPV negative cell line [42].…”

Section: Discussionmentioning

confidence: 99%

Cervical cancer treatment with a locally insertable controlled release delivery system

Keskar

Mohanty

Gemeinhart

et al. 2006

Journal of Controlled Release

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Local delivery of cancer chemotherapeutics enables sustained drug levels at the site of action thereby reducing systemic side effects. A novel insertable polymeric drug delivery system for cervical cancer treatment is presented. Cisplatin, the first line of therapy employed for cervical cancers, was incorporated in a poly(ethylene-co-vinyl acetate) (EVAc) device that is similar to those currently used for vaginal contraceptive delivery. Cisplatin crystals were uniformly dispersed in the polymeric system without undergoing significant dissolution in the polymer matrix. Cisplatin dissolution from the devices was biphasic, consistent with a matrix-type controlled-release system with an initial rapid release phase followed by a slower, linear release phase. Depending on the drug loading in the polymeric devices, the near-linear release phase varied in rate according both empirical, linear curvefitting (0.38±0.15 μg/day to 46.9±10.0 μg/day) and diffusion analysis based upon diffusion through a porous structure (D app from 1.3±0.5×10 −9 cm 2 /s to 5.8±0.3×10 −12 cm 2 /s). The devices were tested for in vitro activity and found to be effective against both HPV positive and HPV negative cervical cancer cell lines. Preliminary studies indicate that this delivery system would be a good candidate for investigation as a choice of treatment in cervical cancers.

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Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix

Cited by 256 publications

References 13 publications

TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells

TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells

Characterization of primary human fibroblasts transformed by human papilloma virus type 16 and herpes simplex virus type 2 DNA sequences

Cervical cancer treatment with a locally insertable controlled release delivery system

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