Human papillomavirus testing and molecular markers of cervical dysplasia and carcinoma

Dehn, Donna L.; Torkko, Kathleen C.; Shroyer, Kenneth R.

doi:10.1002/cncr.22425

Cited by 92 publications

(70 citation statements)

References 141 publications

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“…Therefore, overexpression of p16 INK4a was higher in HPV-positive vs. HPV-negative cases. Moreover, overexpression of p16 INK4a was found to have a higher prevalence in cervical vs. penile cancer (30,31). In our data, no significant association of a p16 INK4a (Fig.…”

Section: Discussionmentioning

confidence: 44%

Relationship among human papillomavirus infection, p16INK4a, p53 and NF-κB activation in penile cancer from northern Thailand

Senba¹,

Mori²,

Fujita³

et al. 2010

Oncology Letters

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Abstract. Human papillomavirus (HPV) E6 and E7 oncoproteins are essential factors for HPV oncogenesis. These E6 and E7 gene products play a central role in the induction of malignant transformation by interacting with several cellular regulatory proteins such as p16 INK4a , p53 and nuclear factor κB (NF-κB). In the present study, conducted in northern Thailand, HPV-DNA was detected in penile cancer cases using an in situ hybridization procedure and p16 INK4a , p53 and NF-κB were detected by immunohistochemistry. Using the cell cycle regulatory proteins p16 INK4a (61.5%) and p53 (71.8%), it was found that of the 51 cases, 39 (76.5%) were HPV-DNApositive in penile cancer. On the other hand, 25% p16 INK4a and 75% p53, respectively, were found in HPV-negative cases. Prevalence of HPV infection (76.5%) was shown in penile cancer cases in northern Thailand. No difference was found between HPV-positive and HPV-negative cases with respect to the presence of the cell cycle regulatory protein p53. On the other hand, p16 INK4a was found to be different between HPV-positive and HPV-negative cases. Inactivation of tumor suppressor genes, such as p16 INK4a and p53, to genetic instability, cell immortalization, accumulation of mutations and cancer formation, with or without HPV and irrespective of HPV infection, is therefore suggested. Of the 39 HPV-positive cases, 35 (89.7%) were NF-κB-positive in the nucleus, 29 (74.4%) in the cytoplasm and 37 (94.9%) in the nucleus and/or cytoplasm. NF-κB was detected in 4 (33.3%) of the 12 HPV-negative cases. Therefore, we propose that penile cancer cases with HPV infection are more likely to activate NF-κB than those without HPV infection.

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Section: Discussionmentioning

confidence: 44%

Relationship among human papillomavirus infection, p16INK4a, p53 and NF-κB activation in penile cancer from northern Thailand

Senba¹,

Mori²,

Fujita³

et al. 2010

Oncology Letters

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“…Cervical cancer associated with HPV, therefore, has increased p16 expression 29,30 and its expression is used by some as a surrogate marker for HPV infection in cervical lesions. [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] It has been hypothesized that HPV infection might similarly play a role in the development of urinary bladder squamous cell carcinoma. In contrast to our findings and the previously mentioned reports, others have shown a positive correlation between high-risk HPV infection and high-grade urothelial cancers of the bladder.…”

Section: Resultsmentioning

confidence: 99%

p16 expression is not associated with human papillomavirus in urinary bladder squamous cell carcinoma

Alexander

Kum

et al. 2012

Modern Pathology

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Squamous cell carcinoma of the urinary bladder is unusual and of unknown etiology. There is a wellestablished association between human papillomavirus (HPV) infection and the development of cervical and head/neck squamous cell carcinomas. However, the role of HPV in the pathogenesis of squamous cell carcinoma of the urinary bladder is uncertain. The purposes of this study were to investigate the possible role of HPV in the development of squamous cell carcinoma of the urinary bladder and to determine if p16 expression could serve as a surrogate marker for HPV in this malignancy. In all, 42 cases of squamous cell carcinoma of the urinary bladder and 27 cases of urothelial carcinoma with squamous differentiation were investigated. HPV infection was analyzed by both in situ hybridization at the DNA level and immunohistochemistry at the protein level. p16 protein expression was analyzed by immunohistochemistry. HPV DNA and protein were not detected in 42 cases of squamous cell carcinoma (0%, 0/42) or 27 cases of urothelial carcinoma with squamous differentiation (0%, 0/15). p16 expression was detected in 13 cases (31%, 13/42) of squamous cell carcinoma and 9 cases (33%, 9/27) of urothelial carcinoma with squamous differentiation. There was no correlation between p16 expression and the presence of HPV infection in squamous cell carcinoma of the bladder or urothelial carcinoma with squamous differentiation. Our data suggest that HPV does not play a role in the development of squamous cell carcinoma of the urinary bladder or urothelial carcinoma with squamous differentiation. p16 expression should not be used as a surrogate marker for evidence of HVP infection in either squamous cell carcinoma of the urinary bladder or urothelial carcinoma with squamous differentiation as neither HVP DNA nor protein is detectable in these neoplasms.

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“…Bunlar; kanser açısından düşük riskli HPV'ler (6,11,40,42,43,44,54, 55 ve 62), olası yüksek riskli HPV'ler (26,53 ve 66) ve yüksek riskli HPV'ler (16 başta olmak üzere 18,31,33,35,39,45,51, 56, 58, 59, 68, 73 ve 82) olarak gruplandırılmaktadırlar 6,7 .…”

Section: Human Papillomavirus (Hpv) Is a Double-strandedunclassified

Human Papillomavirus

Avcı¹,

Bozdayı²

2013

Kafkas J Med Sci

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Human papillomavirus (HPV) is a double-stranded. Diğer birçok virüsün aksine HPV'ler, antijenik yapı-larından çok DNA yapısına göre sınıfl andırıldığından serotipler yerine genotipler olarak ve keşfedildikleri sıraya göre numaralandırılmaktadır 2,4 . Günümüzde 200'den fazla HPV tipi tanımlanmıştır. HPV'lerin sı-nıfl andırılmasında; tür orijini ve DNA hibridizasyonuyla tespit edilen viral genomlar arasındaki homolojinin derecesi önemlidir. DNA sekanslarına göre de papilloma virüslerin fi logenetik sınıfl andırması yapıl-mıştır. Papilloma virüslerin tiplerinin, alt tiplerinin ve varyantlarının taksonomik sınıfl andırılmasında ise major viral protein L1 gen bölgesinin homolojisi göz önünde bulundurulmuştur. Birbirinden en uzak tipler de dahi %40 benzerlik görülmektedir 4,5 .İnsan papilloma virüsü tipleri klinik olarak da üç kategoriye ayrılmaktadır. Bunlar; kanser açısından düşük riskli HPV'ler (6,11,40,42,43,44,54, 55 ve 62), olası yüksek riskli HPV'ler (26,53 ve 66) ve yüksek riskli HPV'ler (16 başta olmak üzere 18,31,33,35,39,45,51, 56, 58, 59, 68, 73 ve 82) olarak gruplandırılmaktadırlar 6,7 . HPV Genomu, Proteinleri ve ReplikasyonuPapilloma virüsler ikozahedral simetrili ve genomu çevreleyen 72 kapsomerli zarfsız DNA virüsleri olup,

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Human papillomavirus testing and molecular markers of cervical dysplasia and carcinoma

Cited by 92 publications

References 141 publications

Relationship among human papillomavirus infection, p16INK4a, p53 and NF-κB activation in penile cancer from northern Thailand

Relationship among human papillomavirus infection, p16INK4a, p53 and NF-κB activation in penile cancer from northern Thailand

p16 expression is not associated with human papillomavirus in urinary bladder squamous cell carcinoma

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