Human Papillomavirus Testing and Conventional Pap Smear Cytology as Optional Screening Tools of Women at Different Risks for Cervical Cancer in the Countries of the Former Soviet Union

Syrjänen, Stina; Shabalova, Irena; Petrovichev, Nicolaj; Козаченко, В. П.; Zakharova, T.; Pajanidi, Julia; Podistov, Jurij; Chemeris, Galina; Sozaeva, Larisa; Lipova, Elena; Tsidaeva, Irena; Ivanchenko, Olga; Pshepurko, Alla; Zakharenko, Sergej; Nerovjna, Raisa; Kljukina, Ludmila; Erokhina, Oksana; Branovskaja, Marina; Ņikitina, Maritta; Grunberga, Valerija; Grunberg, Alexandr; Juschenko, Anna; Tosi, Piero; Cintorino, Marcella; Santopietro, Rosa; Syrjänen, Kari

doi:10.1097/00128360-200204000-00006

Cited by 52 publications

(123 citation statements)

References 36 publications

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“…Final pathological diagnoses included HPV-associated lesions in 97 cases, CIN Ⅰ in 221 cases, CIN Ⅱ in 152 cases, CIN Ⅲ in 178 cases, invasive cervical cancer (ICC) in 20 cases and inflammation in 383 cases, respectively. A total of 1,710 women both LBC-and HR-HPV-negative had an extremely low-risk to develop high-grade CINs during the next several years (8). We considered them to be negative for intraepithelial lesions or malignancy (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…According to this threshold value, we could optimally evaluate the sensitivity and specificity for the risk of high-grade cervical lesions. Some studies showed (8,10) that better sensitivity and specificity could not be obtained by increasing the threshold value of RLU/PC. Therefore, HR-HPV DNA tested by HC-Ⅱ had clinical significance at RLU/PC ≥1.…”

Section: Relationship Between the Viral Loads And Cervical Intraepithmentioning

confidence: 99%

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Hybrid capture II for high-risk human papillomavirus DNA testing to detect cervical precancerous lesions: A qualitative and quantitative study

Bian¹

2009

Exp Ther Med

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Abstract. Hybrid capture Ⅱ (HC-Ⅱ) is the only technique that can be used in clinical human papillomavirus (HPV) DNA detection. However, there is controversy in regards to how to analyze and assess the viral load of high-risk (HR)-HPV by use of HC-Ⅱ and the relation between viral load and cervical lesions. In this study, we analyzed the results of a sequential screening of outpatients at the Department of Obstetrics and Gynecology of the China-Japan Friendship Hospital, and we aimed to explore the relationship between HR-HPV viral load and the severity of cervical lesions, and to clarify the clinical significance of the titer of HR-HPV DNA determined by HC-Ⅱ. Using HC-II, 2,761 women were screened for HR-HPV DNA combined with cytological testing using liquid-based cytology. All women with HR-HPV-positive results or abnormalities in cytology underwent a cervical biopsy through colposcopy. Cervical biopsies were taken in 1,051 women. The HR-HPV infection rate was 78.35% (76/97) in HPV-associated lesions, 87.33% (193/221) in cervical intraepithelial neoplasia (CIN) Ⅰ, 94.74% (144/152) in CIN Ⅱ, 100% (178/178) in CIN Ⅲ and 100% (20/20) in invasive cervical cancer (ICC), respectively (P<0.05). Based on the criteria of histopathology, the sensitivity of HR-HPV DNA testing by HC-Ⅱ for detecting high-grade cervical lesions was 97.71%, the specificity was 79.64%, the positive-predictive value was 41.06% and the negativepredictive value was 99.59%. The viral loads of HR-HPV DNA were 512.15±764.19 in HPV-associated lesions, 753.95±978.27 in CIN Ⅰ, 871.08±1003.52 in CIN Ⅱ, 603.40±740.25 in CIN Ⅲ and 466.44±673.05 in ICC, respectively. In conclusion, the positive rate of HR-HPV increased significantly in accordance with the severity of cervical lesions. The viral loads of cervical inflammatory lesions were markedly lower than CINs and ICC. The viral loads of HR-HPV DNA tested by HC-Ⅱ had no correlation with the grade of cervical lesions.

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Section: Resultsmentioning

confidence: 99%

Section: Relationship Between the Viral Loads And Cervical Intraepithmentioning

confidence: 99%

Hybrid capture II for high-risk human papillomavirus DNA testing to detect cervical precancerous lesions: A qualitative and quantitative study

Bian¹

2009

Exp Ther Med

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“…20 On the contrary, Castle et al, in a study of 797,927 women, Pap smears and HPV DNA testing for 13 carcinogenic HPV genotypes were performed. 21 The study concluded that the likelihood of testing carcinogenic HPV positive but cytologic negative to be only slightly more common than the likelihood of testing carcinogenic HPV negative but cytologic positive (3.99% versus 2.90%).…”

Section: Discussionmentioning

confidence: 99%

The use of HPV-DNA testing combined with Pap smear in detection of pre-invasive disease of the cervix

Nwesar¹,

Salam²,

Hanafi³

et al. 2017

Int J Reprod Contracept Obstet Gynecol

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Background: Cancer cervix constitutes a major health problem worldwide. It is one of the most common female malignancy in both incidence and mortality. Cancer cervix has many risk factors, the most important one is persistent infection with one of HPV high risk types. Its morbidity and mortality can be reduced by frequent screening and early diagnosis. So that, several studies have been conducted in recent years in order to find better tests for screening for pre-invasive disease of the cervix and so early intervention and better prognosis. This study aimed to compare the sensitivity of Pap test and HPV DNA test as screening tests for pre-invasive disease of the cervix.Methods: 100 females from those attending Alexandria University gynaecologic clinic for causes rather than cancer cervix were subjected to Pap smear and cervical swab for HPV-DNA testing at the same setting. Cases of HSIL or positive HPV were subjected to VIA test, colposcopy and cervical punch biopsy was taken if aceto-whitening of the cervix or any other abnormality was found. Cases with ASCUS or LSIL were re-smeared after 3-6 months, if persistent or progressive pathology, colposcopy and punch biopsy from acetowhite areas were taken.Results: 21 cases (21%) were HPV positive and 66 cases (66%) were positive for intraepithelial lesions (37% ASCUS, 18% LSIL and 11% HSIL) with re-smearing there were 3 persistent ASCUS cases (8.1%) and 5 LSIL cases (27.78%). Colposcopy done, and biopsies were taken from 10 HSIL cases (90.1%), 5 LSIL (27.8%), 3 ASCUS (8.1%) and 10 HPV positive cases (62.5%). With significant relationship between colposcopic findings and HPV-DNA positivity and abnormal cytology. Biopsies were 18; 14 were CIN I and 4 were CIN II.Conclusions: HPV-DNA positivity has positive association with HSIL. Pap smear is an easy cheap method for screening. HPV-DNA test is less sensitive than cytology as a method for screening.

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“…The concordance (intraclass correlation coefficient, weighted j) between the two raters was 0.758 [95% confidence interval (95% CI), 0.684-0.815]. Cervical lesions were graded using the CIN nomenclature, extended by the lowest category (HPV-NCIN), denoting lesions with HPV morphology but devoid of CIN (1,26,27 Follow-up and Viral Outcomes. All women who presented with low-grade lesions (HPV-NCIN or HPV-CIN1) were subjected to prospective follow-up, whereas high-grade lesions were promptly treated as detailed before (28)(29)(30)(31).…”

Section: Methodsmentioning

confidence: 99%

“…12 We recently conducted a cohort study testing 3,187 women for optional screening tools in three New Independent States of the Former Soviet Union, and f900 of these women were followed-up to assess the natural history of their HPV infections (the New Independent States cohort study; refs. 26,27). We also analyzed the temporal relationships between acquisition, persistence, and clearance of high-risk HPV and the clinical outcome of the lesions (28)(29)(30)(31).…”

Section: Introductionmentioning

confidence: 99%

Cell Cycle Regulators p105, p107, Rb2/p130, E2F4, p21CIP1/WAF1, Cyclin A in Predicting Cervical Intraepithelial Neoplasia, High-Risk Human Papillomavirus Infections and Their Outcome in Women Screened in Three New Independent States of the Former Soviet Union

Santopietro

Shabalova²,

Petrovichev³

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: The growth-controlling functions of the highrisk human papillomaviruses (HPV) depend on their ability to interact with several cellular proteins, including the key regulatory proteins of the cell cycle. We have examined the value of cell cycle regulatory proteins as predictors of the intermediate end point markers in cervical carcinogenesis: (a) grade of cervical intraepithelial neoplasia (CIN), (b) highrisk HPV type, (c) clearance/persistence of high-risk HPV, and (d) disease outcome in women participating in a multicenter follow-up study in three New Independent States countries. Methods: Totally, 232 biopsy samples tested high-risk HPVpositive and/or Papanicolaou smear -positive women were immunohistochemically stained for the following cell cycle markers: p105, p107, p130, E2F4, p21 CIP1/WAF1/SDI1 , cyclin A, and Ki-67. In addition, apoptotic index (AI) and mitotic index (MI) were determined in H&E-stained sections. Prospective follow-up data were available to disclose the clinical and virological outcome of the lesions.

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Human Papillomavirus Testing and Conventional Pap Smear Cytology as Optional Screening Tools of Women at Different Risks for Cervical Cancer in the Countries of the Former Soviet Union

Cited by 52 publications

References 36 publications

Hybrid capture II for high-risk human papillomavirus DNA testing to detect cervical precancerous lesions: A qualitative and quantitative study

Hybrid capture II for high-risk human papillomavirus DNA testing to detect cervical precancerous lesions: A qualitative and quantitative study

The use of HPV-DNA testing combined with Pap smear in detection of pre-invasive disease of the cervix

Cell Cycle Regulators p105, p107, Rb2/p130, E2F4, p21CIP1/WAF1, Cyclin A in Predicting Cervical Intraepithelial Neoplasia, High-Risk Human Papillomavirus Infections and Their Outcome in Women Screened in Three New Independent States of the Former Soviet Union

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