Human Papillomavirus (HPV) Genotyping of Cutaneous Warts in Greek Children

Abstract

The human papillomavirus (HPV) infects the squamous epithelium of the skin and produces common warts, plantar warts, and flat warts, which occur commonly on the hands, face, and feet. The objective of this study was to determine the presence of HPV in warts in children in order to associate the virus with the disease. Sixty-eight children with clinically diagnosed cutaneous warts were recruited. Skin biopsy samples were examined and DNA was extracted using a commercially available kit. To distinguish between the HPV types, we used a specific pair of primers to amplify the HPV DNA. Polymerase chain reaction amplification of the L1 region was followed by restriction fragment length polymorphism analysis and Luminex xMAP technology. HPV 57 was the predominant type in our study, although the detection of the high-risk HPV type 16 in 33% of our positive samples indicates the presence of mucosal high-risk HPV types in the skin of children. It seems that the newly introduced Luminex assay maximized the discrimination of genotypes even in the case of multiple HPV infections. Or findings also suggest the presence of high-risk HPV types in cutaneous warts.

Kerinokeratosis Papulosa of Childhood

“…HPV-57 is frequently found in viral warts of children [9]. In a recent study performed in elementary schools in the Netherlands, HPV-57 DNA was detected in 14% of all warts.…”

“…As keratotic disorders may result from infection with HPV, histological samples were investigated for the presence of HPV DNA by PCR. Interestingly, sequencing of the PCR products yielded DNA of HPV type 57 (HPV-57), a HPV type which has been described to be prevalent in warts [9]. However, clinical, dermatoscopic and histological examination were not typical for verrucae vulgares or planae.…”

Analgetische Wirkungen lokalanästhetischer Salbe bei der Kryotherapie von Warzen: eine Meta‐analyse randomisierter Studien

“…Warzen entstehen durch eine Infektion mit humanen Papillomaviren (HPV) und gehören zu den häufigsten Hauterkrankungen. Warzen können auf verhornter Haut (wie Handflächen, Fußsohlen und periungual) oder auf nicht verhornder Haut (Genitalbereich) auftreten .…”

Detection of Human Papillomavirus among Patients with Clinically Diagnosed Cutaneous Warts by PCR Method

“…While cutaneous types infect the squamous epithelium of the skin and leads to a diverse range of epithelial lesions, including common warts, plantar warts, flat warts, etc. [3].…”

“…TS-MPG assay can only detect 25 beta-HPV subtypes which predominantly infect skin tissue [5]. Luminex xMap technology allows rapid, sensitive and specific nucleic acid detection for 33 HPV-associated subtypes, again is costly and not readily available in the clinical setting [3,6]. A PCR-based method was developed that different primers were specifically designed based on the relatively conserved regions of the L1 open reading frame of…”