Human papillomavirus prevalence and cytopathology correlation in young Ugandan women using a low‐cost liquid‐based pap preparation

Taube, Janis M.; Kamira, Betty; Motevalli, Mahnaz; Nakabiito, Clemensia; Lukande, Robert; Kelly, Deidra; Erozan, Yener S.; Gravitt, Patti E.; Buresh, Megan; Mmiro, Francis; Bagenda, Danstan; Guay, Laura; Jackson, J. Brooks

doi:10.1002/dc.21249

Cited by 8 publications

(11 citation statements)

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“…Compared to HIV negative women, HIV positive women had more HR-HPV genotypes detected in cervical/vaginal specimens [16-19] and HSIL lesions [17]. The mean number of HPV genotypes detected was higher among HIV positive compared to HIV negative women (2.8; range 1-9) and 2.1; range 1-10, respectively; [ t -test, 3.88; p < 0.001]) [16].…”

Section: Resultsmentioning

confidence: 99%

“…Likewise, among young primiparous women, the mean number of HPV types detected among HIV positive women was higher (2.4, range 1-10) than among HIV negative women (1.8, range 1-12) [Student's t-test = 2.79, p = 0.005] [18]. HIV positive women were four times more likely to have abnormal cytology than HIV negative women (43% vs. 11.6%, p < 0.001) [19]. HPV positive women co-infected with HIV had other HR-HPV genotypes that did not include HPV 16 or 18 [16,17].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Epidemiology of HPV genotypes in Uganda and the role of the current preventive vaccines: A systematic review

Banura

Mirembe

Katahoire

et al. 2011

Infect Agents Cancer

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BackgroundLimited data are available on the distribution of human papillomavirus (HPV) genotypes in the general population and in invasive cervical cancer (ICC) in Uganda. Yet, with the advent of preventive HPV vaccines that target HPV 16 and 18 responsible for causing about 70% of ICC cases in the world, such information is crucial to predict how vaccination and HPV-based screening will influence prevention of ICC.MethodsTo review the distribution of HPV infection and prevalent genotypes, electronic databases (e.g. PubMed/MEDLINE and HINARI) were searched for peer reviewed English articles on HPV infection up to November 30, 2010. Eligible studies were selected according to the following criteria: DNA-confirmed cervical or male genital HPV prevalence and genotypes, HPV incidence estimates and HPV seroprevalence among participants.ResultsTwenty studies were included in the review. Among HIV negative adult women, the prevalence of HR-HPV infections ranged from 10.2% -40.0% compared to 37.0% -100.0% among HIV positive women. Among HIV positive young women aged below 25 years, the prevalence of HR-HPV genotypes ranged from 41.6% -75.0% compared to 23.7% -67.1% among HIV negative women. Multiple infections with non vaccine HR-HPV genotypes were frequent in both HIV positive and HIV negative women. The main risk factors for prevalent HPV infections were age, lifetime number of sexual partners and HIV infection. Incident infections with HR-HPV genotypes were more frequent among adult HIV positive than HIV negative women estimated at 17.3 and 7.0 per 100 person-years, respectively. Similarly, incident HR-HPV among young women aged below 25 years were more frequent among HIV positive (40.0 per 100 person-years) than HIV negative women (20.3 per 100 person-years) women. The main risk factor for incident infection was HIV infection. HPV 16 and 18 were the most common genotypes in ICC with HPV 16/18 contributing up to 73.5% of cases with single infections.Among uncircumcised adult HIV positive males, HR-HPV prevalence ranged from 55.3% -76.6% compared to 38.6% -47.6% in HIV negative males. Incident and multiple HR-HPV infections were frequent in HIV positive males. Being uncircumcised was the main risk factor for both prevalent and incident HPV infection.ConclusionInfections with HR-HPV genotypes were very common particularly among HIV positive individuals and young women irrespective of HIV status. Given the high prevalence of HIV infection, HPV-associated conditions represent a major public health burden in Uganda. However, although the most common HPV genotypes in ICC cases in Uganda were those targeted by current preventive vaccines, there were a large number of individuals infected with other HR-HPV genotypes. Technology allowing, these other HR-HPV types should be considered in the development of the next generation of vaccines.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Epidemiology of HPV genotypes in Uganda and the role of the current preventive vaccines: A systematic review

Banura

Mirembe

Katahoire

et al. 2011

Infect Agents Cancer

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“…Additionally, the sampling protocol may have affected the results. Prior LBC studies have had the advantage of using residual fluid from the initial LBC Pap screening for evaluation of manual LBC methods [9,10,11,12]. However, since we were comparing 2 different LBC methods on women in clinical care, the VitroPrep sample was always taken second to ThinPrep sampling and this may have affected the quality of samples.…”

Section: Discussionmentioning

confidence: 99%

“…The studies that directly compared to conventional cytology reported unsatisfactory slides due to obscuring blood or clumped cells [13] and correlation with conventional cytology was reported as only 68% [14]. The other manual LBC studies describe satisfactory slides for interpretation though there was no comparison with conventional cytology [9,10,11,12]. Another low-cost method of cervical cancer screening used in low-income countries is visual inspection with acetic acid which requires no testing or expertise of a pathologist in making clinical diagnoses.…”

Section: Discussionmentioning

confidence: 99%

“…Manual LBC and conventional cytology are both low-cost procedures at USD 2-3 per slide, especially compared to the infrastructure investment of over USD 55,000 for current automated LBC systems [9]. Other manual LBC methods have been described [9,10,11,12] and compared to conventional cytology [13,14]. The studies that directly compared to conventional cytology reported unsatisfactory slides due to obscuring blood or clumped cells [13] and correlation with conventional cytology was reported as only 68% [14].…”

Section: Discussionmentioning

confidence: 99%

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Manual Liquid-Based Cytology: A Clinical Pilot Study of the VitroPrep™ Cytology Processing Kit

Rahangdale

Budwit²,

Asgari

et al. 2014

Acta Cytologica

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Objective: It was the aim of this study to assess the utility of the manual liquid-based cytology (LBC) product VitroPrep™ Cytology Processing Kit (ChemQ Bioscience LLC, Research Triangle Park, N.C., USA). Study Design: This is a descriptive pilot study. Women underwent cervical sampling processed by the ThinPrep™ automated LBC system followed by cervical sampling for the VitroPrep manual system. The following criteria were assessed on a scale of 1-5 (1 = unsatisfactory, 2 = borderline, 3 = acceptable, 4 = good, 5 = excellent): monolayer cell adhesion, overall cellularity, background clarity, preservation of cellular morphology, red cell lysis, and elimination of mucus/debris. Cytological diagnosis was compared to results from ThinPrep samples. In addition, VitroPrep samples were taken prior to conization procedures and compared to pathology results. Descriptive statistics were performed. Results: Forty-two of 47 women who underwent dual cytologic sampling had satisfactory samples. All scores were 3-5, with >90% graded 4-5. The VitroPrep diagnosis correlated with the ThinPrep diagnosis in 90% (38/42) of cases. All specimens obtained from 15 women prior to conization were satisfactory and correlated abnormal cytologic findings with cervical intraepithelial neoplasia 1-3 pathology. Conclusions: The VitroPrep Cytology Processing Kit was able to provide adequate specimens for evaluation and diagnosis. This low-cost processing kit may provide a useful alternative in settings where automated LBC systems may not be feasible.

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Genotypic diversity of anogenital human papillomavirus in women attending cervical cancer screening in Harare, Zimbabwe

Mandishora

Christiansen

Chin'ombe

et al. 2017

Journal of Medical Virology

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Although anogenital cancers have been on a gradual rise in developing countries in the past few decades, they have been understudied. The objective was to investigate genotypic diversity of anogenital HPV amongst women reporting for routine cervical cancer screening in Harare in Zimbabwe. A cross-sectional study that enrolled 144 women ≥18 years from a cervical cancer-screening clinic was performed. Each woman provided a self-collected cervico-vaginal swab (VS) and a clinician-collected anal swab (CCAS). HIV testing was offered and cervical cytology was performed. Both VS and CCAS samples were HPV genotyped, using amplicon sequencing of the L1 gene region with Illumina technology. Mean age of the women was 39.9 (range 18-83 years, SD ± 11.0). HPV prevalence was 72% (104/144) in VS and 48% (69/144) in CCAS. The most common genotypes detected in both VS and CCAS were HPV18, HPV52, and HPV16. Sixty two percent of the subjects had multiple genotypic HPV infections. The odds of being HPV-positive among HIV-infected women were higher than in HIV-negative women in both the vagina and the anus (CCAS OR = 4.8; CI 2.4-9.8, P < 0.001) and (VS OR = 2.9; CI 1.3-6.4, P = 0.005). High HPV prevalence and diverse genotypes were detected in both the vagina and anus. Anal oncogenic HPV infection was common. HPV 52 was one of the most common oncogenic genotypes in both the vagina and anus. HIV co-infection played a significant role in the prevalence of HPV. These data have implications for design of primary and secondary programs for prevention of anogenital cancer in Zimbabwe.

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Human papillomavirus prevalence and cytopathology correlation in young Ugandan women using a low‐cost liquid‐based pap preparation

Cited by 8 publications

References 50 publications

Epidemiology of HPV genotypes in Uganda and the role of the current preventive vaccines: A systematic review

Epidemiology of HPV genotypes in Uganda and the role of the current preventive vaccines: A systematic review

Manual Liquid-Based Cytology: A Clinical Pilot Study of the VitroPrep™ Cytology Processing Kit

Genotypic diversity of anogenital human papillomavirus in women attending cervical cancer screening in Harare, Zimbabwe

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