Human Papillomavirus‒Positive and ‒Negative Vulvar Squamous Cell Carcinoma Are Biologically but Not Clinically Distinct

Kolitz, Elysha; Lucas, Elena; Hosler, Gregory A.; Kim, Jiwoong; Hammer, Suntrea T.G.; Lewis, Cheryl; Xu, Lin; Day, Andrew T.; Mauskar, Melissa M.; Lea, Jayanthi; Wang, Richard C.

doi:10.1016/j.jid.2021.10.009

Cited by 11 publications

(17 citation statements)

References 58 publications

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“…Endogenous circRNAs are reported to be highly expressed in HPV-positive tumors. 41 In HPV-related malignancies, circRNAs are documented to participate in tumorigenesis, cancer evolution, and drug resistance, among which some are regarded as effective diagnostic and prognostic targets. 42 Furthermore, we found that circMTO1-upregulated CC individuals had a higher occurrence of HR-HPV infection and circMTO1 was independently related to HR-HPV infection in CC, pointing to the strong relevance of circMTO1 and HR-HPV infection.…”

Section: Discussionmentioning

confidence: 99%

“…Based on our results, circMTO1 was much higher in HR-HPV–positive CC patients and its serum level greater than 2.480 (56.41% sensitivity and 100% specificity) exerted a diagnostic value for HR-HPV–positive CC. Endogenous circRNAs are reported to be highly expressed in HPV-positive tumors 41 . In HPV-related malignancies, circRNAs are documented to participate in tumorigenesis, cancer evolution, and drug resistance, among which some are regarded as effective diagnostic and prognostic targets 42 .…”

Section: Discussionmentioning

confidence: 99%

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High Expression of Circular RNA–Mitochondrial tRNA Translation Optimization 1 Assists the Diagnosis of High-Risk Human Papillomavirus Infection in Cervical Cancer

Cheng

Changmei

Zhenrong

2022

J Low Genit Tract Dis

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Objective Persistence of high-risk human papillomavirus (HR-HPV) infection is a paramount determinant in cervical cancer (CC) development. Circular RNAs have the potential to be promising biomarkers for various cancers. This study explored circular RNA–mitochondrial tRNA translation optimization 1 (circMTO1) expression in the serum of CC patients and its clinical value in diagnosing CC and predicting HR-HPV infection. Materials and Methods In total, 125 CC patients (including 78 cases with HR-HPV) were enrolled, with another 76 healthy people as controls. Serum circMTO1 and miR-199a expressions were detected by reverse transcription–quantitative polymerase chain reaction, and the diagnostic efficacy of circMTO1 for CC and HR-HPV infection was analyzed by the receiver operating characteristic curve. According to the median of serum circMTO1 expression, CC patients were assigned into circMTO1 low/high expression groups to analyze the correlation between circMTO1 and clinical parameters using the Fisher and χ 2 tests. Independent association of circMTO1 with HR-HPV infection in CC was evaluated via logistics multivariate regression analysis. Targeted relationship between miR-199a and circMTO1 was predicted by Starbase Web site and validated via dual-luciferase assay, with their correlation further assessed by Pearson analysis. Results Serum circMTO1 was increased in CC patients and prominently elevated in HR-HPV–positive CC patients, with a level greater than 1.485 assisting CC diagnosis and a level greater than 2.480 assisting HR-HPV–positive diagnosis. The circMTO1 was interrelated to clinical stage, tumor differentiation, lymph node metastasis, invasion depth, and independently linked with HR-HPV infection in CC. Serum miR-199a was downregulated in HR-HPV–positive CC patients and inversely correlated with circMTO1. Conclusions Serum circMTO1 is upregulated in HR-HPV–positive CC patients and has a diagnostic value for HR-HPV infection in CC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

High Expression of Circular RNA–Mitochondrial tRNA Translation Optimization 1 Assists the Diagnosis of High-Risk Human Papillomavirus Infection in Cervical Cancer

Cheng

Changmei

Zhenrong

2022

J Low Genit Tract Dis

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“…In a recent RNA sequencing analysis published by Kolitz et al, VSCC were analyzed for differential expressed genes in HPV+ vs. HPV− tumors [ 23 ]. E2F was upregulated in HPV+ cases, following HPV E7 activation, and EYA2 was also upregulated, which has been shown to promote growth and migration in cervical cancer.…”

Section: Discussionmentioning

confidence: 99%

“…Two distinct groups, defined by HPV status, with several differentially expressed genes (e.g., STMN1, FOXO6, FCGBP DMBX1, and GBX1) were detected. In the pathway analysis, enrichment for cytokine receptor and inflammatory signaling was revealed in the HPV+ tumors [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis in Vulvar Squamous Cell Cancer

Alawi

Jaeger

Wankner

et al. 2021

Cancers

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To date, therapeutic strategies in vulvar squamous cell carcinoma (VSCC) are lacking molecular pathological information and targeted therapy hasn’t been approved in the treatment of VSCC, yet. Two etiological pathways are widely accepted: HPV induced vs. HPV independent, associated with chronic skin disease, often harboring TP53 mutations (mut). The aim of this analysis was to analyze the RNA expression patterns for subtype stratification on VSCC samples that can be integrated into the previously performed whole exome sequencing data for the detection of prognostic markers and potential therapeutic targets. We performed multiplex gene expression analysis (NanoString) with 770 genes in 24 prior next generation sequenced samples. An integrative data analysis was performed. Here, 98 genes were differentially expressed in TP53mut vs. HPV+ VSCC, in the TP53mut cohort, where 56 genes were upregulated and 42 were downregulated in comparison to the HPV+ tumors. Aberrant expression was primarily observed in cell cycle regulation, especially in HPV+ disease. Within the TP53mut group, a distinct cluster was identified that was correlated to a significantly worse overall survival (p = 0.017). The RNA expression profiles showed distinct patterns with regard to the known VSCC subtypes and could potentially enable further subclassification in the TP53mut groups

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“…Publicly available Sequence Read Archive (SRA) data and The Cancer Genome Atlas (TCGA) sequencing data demonstrate that circE7 is present in both cervical cancer and head and neck squamous cell carcinoma (HNSCC). More recent analyses have demonstrated circE7 to be present in anal squamous cell cancer ( 14 ) and vulvar squamous cell cancer ( 15 ). The proportion of back-spliced reads corresponding to circE7 varied between samples.…”

Section: Lettermentioning

confidence: 99%

Assessment of the Abundance and Potential Function of Human Papillomavirus Type 16 Circular E7 RNA

Wang

Lee

Kim

2022

mBio

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Human Papillomavirus‒Positive and ‒Negative Vulvar Squamous Cell Carcinoma Are Biologically but Not Clinically Distinct

Cited by 11 publications

References 58 publications

High Expression of Circular RNA–Mitochondrial tRNA Translation Optimization 1 Assists the Diagnosis of High-Risk Human Papillomavirus Infection in Cervical Cancer

High Expression of Circular RNA–Mitochondrial tRNA Translation Optimization 1 Assists the Diagnosis of High-Risk Human Papillomavirus Infection in Cervical Cancer

Transcriptome Analysis in Vulvar Squamous Cell Cancer

Assessment of the Abundance and Potential Function of Human Papillomavirus Type 16 Circular E7 RNA

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