Human Papillomavirus Outcomes in an Access‐to‐Care Laryngeal Cancer Cohort

Abstract

Objective Human papillomavirus (HPV), particularly HPV16, is a causative agent for 25% of head and neck squamous cell cancer, including laryngeal squamous cell cancer (LSCC). HPV positive (HPV+ve) patients, particularly oropharyngeal SCC, have improved prognosis. For LSCC, this remains to be established. The goal was to determine stage and survival outcomes in LSCC in the context of HPV infection. Study Design Historical cohort study. Setting Primary care academic health system. Subjects and Methods In 79 primary LSCC, HPV was determined using real-time quantitative PCR. Chi-square or Fisher’s exact test was used to test association of HPV+ve with 21 risk factors including race, stage, gender, age, smoking, alcohol, treatment, and health insurance. Kaplan-Meier and log rank test were used to study the association of HPV and LSCC survival outcome. Results HPV16 was detected in 27% LSCC. There was a trend towards higher HPV prevalence in Caucasian American (CA, 33%) vs African American (AA, 16%) (p=0.058). HPV was significantly associated with gender (p=0.016) and insurance type (p=0.001). HPV+ve LSCC had a slightly longer survival than HPV-negative (HPV−ve) patients, but the differences were not significant. There was no association with HPV and other risk factors including stage (early vs late). Conclusion We found high prevalence of HPV in males and lower prevalence of HPV infection in AA compared to CA. A slightly better survival for HPV+ve LSCC versus HPV−ve was noted but was not significant. Larger multi ethnic LSCC cohorts are needed to more clearly delineate HPV related survival across ethnicities.

Human Papillomavirus in Non-Oropharyngeal Head and Neck Cancers: A Systematic Literature Review

“…Importantly, only four studies [8,9,29,88,95] examined the impact of HPV on the outcome of a total of 319 patients; 134 of which were HPVpositive. No association of HPV status with outcome was found.…”

IGSF4Methylation as an Independent Marker of Human Papillomavirus–Positive Oropharyngeal Squamous Cell Carcinoma

“…16 The cutoff value for HPV16-positive status was ≥0.03 (≥3 HPV genome copies/100 cells). 16,18,19 Bisulfite Modification and Quantitative Methylation-Specific Polymerase Chain Reaction (QMSP) Assay…”

HPV Prevalence in a Multi‐ethnic Screening Population

“…In a pooled meta analysis study 7 using conventional PCR based assays, prevalence of HPV16 in LSCC was 17%. 7 In a recent study, HPV16, detected by real-time quantitative PCR, had a reported prevalence of 27%, supporting a role for HPV in LSCC 28 .…”

“…Specific primers and probes were designed to amplify a housekeeping gene, β-globin 13 (to standardize the input DNA), and the E6 region of HPV16 14 as previously described. 15 DNA amplifications were carried out in a 96-well reaction plate format in an Applied Biosystems 7900HT Sequence Detector (Applied Biosystems, Foster City, CA). Multiple water blanks were included in every run.…”