Human Papillomavirus Infection in Papillomas and Nondiseased Respiratory Sites of Patients With Recurrent Respiratory Papillomatosis Using the Polymerase Chain Reaction

Smith, Elaine; Pignatari, Shirley Shizue Nagata; Gray, Steven D.; Haugen, Thomas H.; Turek, Lubomír P.

doi:10.1001/archotol.1993.01880170080017

Cited by 78 publications

(50 citation statements)

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“…Nevertheless, the lack of methylation within our series of HPV6 URRs was unexpected, since a high frequency of hypermethylation has been observed in host tumor suppressor genes in laryngeal papillomas (25). However, HPV6 has also been detected in nondiseased respiratory sites (17,22), and it is tempting to speculate that expression of HPV6 is repressed by methylation in such tissues. In lesions characterized by koilocytes, a hallmark of permissive HPV infections, it has been shown that HPV16 URR methylation varies depending on the degree of differentiation of the squamous epithelium (28).…”

mentioning

confidence: 66%

Lack of Methylation in the Upstream Region of Human Papillomavirus Type 6 from Aerodigestive Tract Papillomas

Ure

Forslund

2012

J Virol

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Methylation of the high-risk human papillomavirus type 16 (HPV16) upstream regulatory region (URR) has been described, but whether methylation is present among low-risk HPVs is unknown. The methylation status of the HPV6 URR was analyzed in papillomas from the upper aerodigestive tract of six adult patients. All CpGs in the URR were unmethylated, from both basal/ intermediate and superficial cells, suggesting that methylation is not involved in the regulation of transcription from the HPV6 URR, regardless of epithelial differentiation.

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mentioning

confidence: 66%

Lack of Methylation in the Upstream Region of Human Papillomavirus Type 6 from Aerodigestive Tract Papillomas

Ure

Forslund

2012

J Virol

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“…The detection of HPV DNA in tumor-free tonsils has been reported in only a few studies (4,19,23,25,26), and others failed to detect the viral DNA in tonsillitis samples (2,8,12,21). The overall frequency of HPV DNA in normal tonsillar mucosa or benign tonsillar lesions is 8.5% (17 of 200, compiled from several studies), consisting of 70% with HPV-16 and 30% with HPV-6/11, as summarized in a recent review (24).…”

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confidence: 99%

Presence of DNA of Human Papillomavirus 16 but No Other Types in Tumor-Free Tonsillar Tissue

et al. 2005

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According to PCR, the prevalences of human papillomavirus (HPV) DNA were 6.3% (13 of 206) in tonsillitis or hypertrophic tonsillar tissues and 0.6% (1 of 174) in exfoliated cells from normal tonsils. HPV-16 was the only type detected in tonsillar tissues, but it did not appear to lead to L1 antibody production.Of the squamous cell carcinomas of the head and neck, human papillomavirus (HPV) DNA has been repeatedly reported in tonsillar carcinoma, suggesting a role for HPV in tonsillar carcinogenesis (5,9,10,13,22). The reason for the strong association of HPV with tonsils remains unclear. Previous data on the presence of HPV DNA in tumor-free tonsils are based on small series of experiments (24). A significantly increased risk for squamous cell carcinomas of the head and neck is associated with HPV-16 seropositivity (11), but serum antibodies against HPV-16 proteins have been detected in healthy individuals as well (6). Our aim was to study HPV in tumor-free tonsils and to correlate these findings with the seroprevalence of HPV antibodies.We enrolled 212 patients operated on in 2001 and 2002 because of tonsillitis (n ϭ 135) or tonsillar hypertrophy (n ϭ 77) at Helsinki University Central Hospital (Table 1). After tonsillectomy, a piece of tissue was immediately frozen at Ϫ70°C. A 100-m cryo-section per tonsil per patient was subjected to DNA extraction. Five-micrometer sections from both ends of the tissue were toluidine blue stained to verify that the tissue section consisted of at least 30% epithelial cells. After every tenth sample, only optimal cutting temperature compound without tonsillar tissue was cryo-sectioned as a negative control for the DNA extraction; none of these sections showed positive ␤-globin or HPV by PCR. Serum samples were drawn on the day of surgery.Tonsillar exfoliated cells from 189 control subjects (age and sex matched to patients) with normal tonsils were collected bilaterally from tonsillar surfaces with a Cytobrush Plus Cell Collector (Medscand Medical). The brushed samples were stored in phosphate-buffered saline at Ϫ70°C. Serum samples were collected on the same day.DNA was extracted from tonsillar tissue with the DNA mini kit (QIAGEN) and from exfoliated cells with the MagNA Pure LC DNA isolation kit I (Roche). In order to assess the quality of the DNA submitted to HPV PCR, we applied ␤-globin PCR (15) and found that 97% (206 of 212) of DNA samples from tonsillar tissue and 92% (174 of 189) of DNA samples from tonsillar exfoliated cells were positive for ␤-globin.HPV DNA was identified by nested PCR with consensus primers MY09/11 (14) and GP5ϩ/6ϩ (3). The genotype was determined by direct sequencing of the approximately 150-bp amplicons with an ABI PRISM Dye Terminator and analyzed on an ABI 377 DNA sequencer (Applied Biosystems) and on the basis of Ͼ90% homology with HPV sequences deposited in GenBank with BLAST software.Serum antibodies against HPV-16 L1, E6, and E7 were analyzed by a newly developed method. The HPV proteins were expressed as fusion proteins with N-terminal glut...

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“…The virus has several antigenic subtypes. It has been reported that low-risk (i.e., low malignant potential) HPV types 6 and 11 cause about 90% of cases of recurrent respiratory papillomatosis [1,4]. Their existence as latent infections in morphologically normal tissue of the airway and are believed to be the source of recurrent disease [10,11].…”

Section: Discussionmentioning

confidence: 99%

“…Recurrent respiratory papillomatosis (also known as laryngotracheal papillomatosis) is a rare disease characterized by multiple papillomas seen in upper airways, usually on the vocal cords [1,2]. They have been divided into juvenile and adult types, because of their distinct clinical courses and different incidence of malignant transformation, according to the age at onset and the presence or absence of laryngeal involvement [3].…”

Section: Introductionmentioning

confidence: 99%

“…They have been divided into juvenile and adult types, because of their distinct clinical courses and different incidence of malignant transformation, according to the age at onset and the presence or absence of laryngeal involvement [3]. Multiple papillomas are associated with Human papilloma virus (HPV) infection and it has been reported that low-risk (i.e., low malignant potential) HPV types 6 and 11 cause about 90% of cases of recurrent respiratory papillomatosis [1,4]. The primary therapeutic method is endoscopic surgery, and approximately one-tenth of patients are cured after the first surgical intervention.…”

Section: Introductionmentioning

confidence: 99%

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Management of Adult Recurrent Respiratory Papillomatosis with Oral Acyclovir Following Micro Laryngeal Surgery: A Case Series

Chaturvedi

Sreenivas

Hemanth

et al. 2012

Indian J Otolaryngol Head Neck Surg

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To demonstrate the role of oral acyclovir in monthly regimes after microdebrider assisted excision in 3 patients with adult recurrent respiratory papillomatosis (ARRP). Three patients with ARRP who presented to a tertiary referral hospital in stridor were initially treated with a tracheostomy in order to secure airway. On further evaluation by videolaryngoscopy extensive bilateral laryngeal papillomatosis was noted with history of similar conditions in the past for which they were repeatedly operated. They were admitted and underwent Microlaryngeal surgery and laryngeal microdebrider assisted surgery under general anesthesia. Post operatively a course of oral acyclovir at 800 mg/5 times/day for 5 days was administered. On repeat assessment with videolaryngoscopy at monthly intervals a complete remission of the disease was noted with no residual disease at the end of 1 year in 2 cases. One case had a recurrence. Renal parameters were monitored periodically. It may be concluded that the action of anti viral drugs at regular intervals in addition to a short course of oral steroids lead to rapid recovery and prevented latent virus activation within the laryngo tracheal system hence maintaining long term improvement. This can avoid multiple laryngeal surgeries, repeated respiratory emergencies and risk for malignant transformation in the future thereby reducing morbidity and effect on quality of life.

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Human Papillomavirus Infection in Papillomas and Nondiseased Respiratory Sites of Patients With Recurrent Respiratory Papillomatosis Using the Polymerase Chain Reaction

Cited by 78 publications

References 10 publications

Lack of Methylation in the Upstream Region of Human Papillomavirus Type 6 from Aerodigestive Tract Papillomas

Lack of Methylation in the Upstream Region of Human Papillomavirus Type 6 from Aerodigestive Tract Papillomas

Presence of DNA of Human Papillomavirus 16 but No Other Types in Tumor-Free Tonsillar Tissue

Management of Adult Recurrent Respiratory Papillomatosis with Oral Acyclovir Following Micro Laryngeal Surgery: A Case Series

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