Human papillomavirus in upper digestive tract tumors from three countries

Abstract

Based on our results, we cannot deny the possibility of HPV16 involvement in the carcinogenesis of the esophagus.

Human Papillomavirus and Carcinogenesis in the Upper Aero-Digestive Tract

“…Similar trends were observed in ECs although the difference was not statistically significant. Among HPV-16 intratypes, there is one polymorphism in the sequence of the E6 probe at nucleotide 145, and the Asian-American variant harbors this nucleotide substitution (C to T) [33]. However, this polymorphism is unlikely to cause a difference in viral load because the copy number of HPV-16 in the Asian-American variant was similar to other intratypes except E-350G.…”

“…the hybridization event) is amplified and ultimately visualized with one of the various available methodologies. The liquid-phase signal amplification method the Digene Hybrid Capture 2 (HC2) assay [58] (Qiagen, Gaithersburg, MD, USA), is the first Our studies showed that the HPV genome was detected in 56% and 19% of SCCs of the oral cavity and esophagus, respectively, in cases collected from Japan, Pakistan and Colombia and the HPV prevalence in both oral cancers (OCs) and esophageal cancers (ECs) did not significantly differ by country [33]. HPV16 was frequently integrated into the host genome in patients with OCs and ECs, however, the viral loads in these malignancies were much lower than those found in cancer of the cervix [33].…”

“…The liquid-phase signal amplification method the Digene Hybrid Capture 2 (HC2) assay [58] (Qiagen, Gaithersburg, MD, USA), is the first Our studies showed that the HPV genome was detected in 56% and 19% of SCCs of the oral cavity and esophagus, respectively, in cases collected from Japan, Pakistan and Colombia and the HPV prevalence in both oral cancers (OCs) and esophageal cancers (ECs) did not significantly differ by country [33]. HPV16 was frequently integrated into the host genome in patients with OCs and ECs, however, the viral loads in these malignancies were much lower than those found in cancer of the cervix [33]. It should be noted, however, that human cancer is often regarded as a stem cell-like disease originating from a small fraction of cancer cells that show self-renewal and pluripotency and are capable of initiating and sustaining tumor growth [59].…”

“…Also, sequencing of an amplified L1 gene fragment was used to identify HPV genotype. In other studies in the UADT using formalin-fixed and paraffin-embedded specimens [32,33], we used an ultrasensitive short-fragment PCR assay, the SPF10, which amplifies a 65 base pair region within L1 [56]. The HPV types were determined using the INNO-LiPA HPV genotyping v2 kit (Innogenetics NV, Belgium), which is based on the reverse hybridization principle.…”

“…The highrisk type HPV-16 was the most prevalent type with a viral load in SCC of the tonsil similar to that of cervical cancer ( Table 1). The HPV-16 genomes detected in SCCs of the oral cavity, oropharynx and esophagus were frequently integrated in the host genome [33].…”