Human papillomavirus (HPV) DNA detection in self-collected urine

Prusty, Bhupesh K.; Kumar, Arunaz; Arora, Raksha; Batra, Swaraj; Das, B. C.

doi:10.1016/j.ijgo.2005.06.004

Cited by 45 publications

(46 citation statements)

References 23 publications

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“…Midstream urine-i.e., the standard for the diagnosis of metabolic diseases and urinary tract infections-is preferred by some authors, since it is thought to contain less PCR inhibitors. Three studies using midstream urine did not report on human DNA detection [45,46,50].…”

Section: Urine Samplingmentioning

confidence: 99%

Detection of human papillomavirus DNA in urine. A review of the literature

Vorsters

Micalessi

Bilcke

et al. 2011

Eur J Clin Microbiol Infect Dis

114

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The detection of human papillomavirus (HPV) DNA in urine, a specimen easily obtained by a non-invasive self-sampling method, has been the subject of a considerable number of studies. This review provides an overview of 41 published studies; assesses how different methods and settings may contribute to the sometimes contradictory outcomes; and discusses the potential relevance of using urine samples in vaccine trials, disease surveillance, epidemiological studies, and specific settings of cervical cancer screening. Urine sampling, storage conditions, sample preparation, DNA extraction, and DNA amplification may all have an important impact on HPV DNA detection and the form of viral DNA that is detected. Possible trends in HPV DNA prevalence in urine could be inferred from the presence of risk factors or the diagnosis of cervical lesions. HPV DNA detection in urine is feasible and may become a useful tool but necessitates further improvement and standardization.

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Section: Urine Samplingmentioning

confidence: 99%

Detection of human papillomavirus DNA in urine. A review of the literature

Vorsters

Micalessi

Bilcke

et al. 2011

Eur J Clin Microbiol Infect Dis

114

View full text Add to dashboard Cite

show abstract

“…Unlike cytology, HPV testing is amenable to simplified approaches for sample collection, including self-collected vaginal samples and urine-based sampling. These approaches may offer alternatives to clinic-based screening and improve access to care, particularly for hard-to-reach populations (2)(3)(4)(5)(6)(7)(8)(9)(10)(11). While urine-based screening offers simplicity in sample collection, there are few studies of clinical performance of urine-based sampling and HPV genotypes detectable by this approach compared to that of cervical and external genital sampling in relation to rigorously confirmed cervical disease status.…”

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confidence: 99%

Comparison of Human Papillomavirus Detections in Urine, Vulvar, and Cervical Samples from Women Attending a Colposcopy Clinic

et al. 2014

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e While urine-based sampling for human papillomavirus (HPV) is being explored as a simple and noninvasive approach for cervical cancer screening, data comparing HPV genotyping in urine and those in cellular sampling of the cervix and vulva, and their correlation with rigorously confirmed cervical disease status, are sparse. We performed HPV genotyping on voided-urine and clinician-collected vulvar and cervical samples from 72 women undergoing colposcopy. Although urine-based HPV carcinogenic HPV detection was lower (58.3%) than cervical (73.6%) and vulvar (72.1%) detection (P ‫؍‬ 0.05 and 0.07, respectively), the agreement of urine HPV with cervical and vulvar HPV was moderate (kappa ‫؍‬ 0.55) and substantial (kappa ‫؍‬ 0.62), respectively. Urine-based carcinogenic HPV detection had a clinical sensitivity of 80.8% (95% confidence interval [CI] ‫؍‬ 60.7 to 93.5) and a specificity of 53.3% (95% CI ‫؍‬ 37.9 to 68.3) for diagnosing cervical intraepithelial neoplasia grades 2/3 (CIN2/3) on histology; 90.0% of CIN3 was positive for urine HPV. The corresponding sensitivity and specificity values for vulvar sampling were 92% (95% CI ‫؍‬ 74 to 99) and 40.5% (95% CI ‫؍‬ 25.6 to 56.7), and those for cervical sampling were 96.2% (95% CI ‫؍‬ 80.4 to 99.9) and 40% (95% CI ‫؍‬ 25.7 to 55.7), respectively. HPV16 was the most common carcinogenic genotype detectable in 25% of urine, 33.8% of vulvar, and 31.9% of cervical samples overall, with prevalence increasing with cervical disease grade, regardless of the sampling method. Stronger cervical HPV PCR signal strengths were associated with increased frequency of urine HPV detection. In summary, the relatively lower detection rates but comparable clinical performance of urine-based HPV sampling underscore the need for larger studies to evaluate urine-based sampling for cervical cancer screening, epidemiologic studies, and postvaccination HPV disease surveillance. Human papillomavirus (HPV)-based primary screening or cotesting is being increasingly implemented as an alternative to cytology-based screening (1). Unlike cytology, HPV testing is amenable to simplified approaches for sample collection, including self-collected vaginal samples and urine-based sampling. These approaches may offer alternatives to clinic-based screening and improve access to care, particularly for hard-to-reach populations (2-11). While urine-based screening offers simplicity in sample collection, there are few studies of clinical performance of urine-based sampling and HPV genotypes detectable by this approach compared to that of cervical and external genital sampling in relation to rigorously confirmed cervical disease status. To expand the evidence in this area, we conducted a pilot study in a colposcopy referral population to evaluate HPV detection in voided urine samples, compare it to clinician-collected external genital (vulvar) and cervical sampling, and correlate results against histologically confirmed cervical disease ascertainment. MATERIALS AND METHODSThe study was nested within the National Ca...

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“…All the cervical samples collected from Perdoor women were HPV DNA positive showing 100%concordance. Another study by Prusty et al also found 100% agreement in HPV positivity between cervical and urine samples (Prusty et al, 2005). A metaanalysis study comparing the sensitivity and specificity of Pap smear and HPV DNA found better sensitivity and specificity of HPV-DNA testing in the order of 94.6% and 94.2% (Koliopoulos et al, 2007).…”

Section: Discussionmentioning

confidence: 94%

Detection of Genital HPV Infection Using Urine Samples: a Population Based Study in India

Sabeena

Bhat²,

Kamath

et al. 2016

Asian Pacific Journal of Cancer Prevention

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Background: Cervical cancer is the second commonest cancer among Indian women and its association with human papilloma virus (HPV) is well established. This preventable cancer accounts for the maximum number of cancer related deaths among rural Indian women. Unlike in developed countries there are no organized cervical cancer screening programmes in India due to lack of resources and manpower. Objective: To detect genital HPV infection using urine samples among asymptomatic rural women in the age group of 18-65 years. Materials and Methods: The study area chosen was Perdoor village in Udupi Taluk, Karnataka State and all the women in the age group of 18-65 years formed the study cohort. A cross sectional study was conducted by house visits and 1,305 women were enrolled in the study. After taking written informed consent a data sheet was filled and early stream random urine samples were collected, transported to a laboratory at 4 O C and aliquoted. Samples were tested using nested HPV PCR with PGMY09/11 and GP5+/6+ primers. Positive cases were genotyped by sequence analysis. Results: Study participants included 1,134 sexually active and 171 unmarried women with a mean age at marriage of 22.1 (SD=3.9) years. Study area showed high female literacy rate of 86.6%. Five urine samples tested positive for HPV DNA (0.4%). Conclusions: We found very low genital HPV infection rate among women from monogamous community. This is the first major population based study carried out among asymptomatic rural women to detect genital HPV infectio from Karnataka using urine samples.

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Human papillomavirus (HPV) DNA detection in self-collected urine

Cited by 45 publications

References 23 publications

Detection of human papillomavirus DNA in urine. A review of the literature

Detection of human papillomavirus DNA in urine. A review of the literature

Comparison of Human Papillomavirus Detections in Urine, Vulvar, and Cervical Samples from Women Attending a Colposcopy Clinic

Detection of Genital HPV Infection Using Urine Samples: a Population Based Study in India

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