Human papillomavirus genotypes distribution in cervical samples from women living with human immunodeficiency virus

Melgaço, Fabiana Gil; Rosa, Maria Luiza Garcia; Augusto, Everton Faccini; Haimuri, Jacqueline; Jacintho, Claudia; Santos, Larissa Silva; Cavalcanti, Sílvia M. B.; Oliveira, Ledy H. S.

doi:10.1007/s00404-010-1443-z

Cited by 28 publications

(29 citation statements)

References 40 publications

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“…In these regions, HIV+ women are has better follow-up statistics and correctly use the HAART, which seems to protect against the development of cervical lesions (Rocha-Brischiliari et al, 2014). The prevalence of low-grade cervical abnormalities is common in younger patients (Kurman et al, 2011), which is in agreement with these studies once the median age of HIV+ and HIV-women was below 35 years old (Entiauspe et al, 2010;Oliveira et al, 2010;Andrade et al, 2011;Melgaco et al, 2011;.…”

Section: Prevalence Of Cervical Abnormalities In Hiv+ Brazilian Womenmentioning

confidence: 61%

“…As expected, studies performed to detection HPV at the molecular level together with cervical cytology and/ or histopathology showed an association of HR-HPV with cervical abnormalities (Mattos et al, 2011), mostly HSIL (Melgaco et al, 2011) or CIN II/III and SCC (Andrade et al, 2011).…”

Section: Prevalence Of Cervical Abnormalities In Hiv+ Brazilian Womenmentioning

confidence: 63%

“…HPV-16 and -6 were the most frequent genotypes, and a high prevalence of HPV co-infections were also detected (64.8%). Melgaco et al (2011) evaluated HIV+ women, the majority of whom were treated with HAART. The prevalence of HPV was 60.0%, and HPV-16 was the most prevalent, followed by other HR-HPV genotypes such as HPV-53 and -58.…”

Section: Molecular Prevalence Of Hpv and Genotypes In Hiv+ Women Accomentioning

confidence: 99%

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HPV Infection and Cervical Abnormalities in HIV Positive Women in Different Regions of Brazil, a Middle-Income Country

Freitas

Suehiro²,

Consolaro³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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Human papillomavirus is a virus that is distributed worldwide, and persistent infection with high-risk genotypes (HR-HPV) is considered the most important factor for the development of squamous cell cervical carcinoma (SCC). However, by itself, it is not sufficient, and other factors may contribute to the onset and progression of lesions. For example, infection with other sexually transmitted diseases such as human immunodeficiency virus (HIV) may be a factor. Previous studies have shown the relationship between HPV infection and SCC development among HIV-infected women in many regions of the world, with great emphasis on low-and middleincome countries (LMICs). Brazil is considered a LMIC and has great disparities across different regions. The purpose of this review was to highlight the current knowledge about HPV infection and cervical abnormalities in HIV+ women in Brazil because this country is an ideal setting to evaluate HIV impact on SCC development and serves as model of LMICs and low-resource settings.

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Section: Prevalence Of Cervical Abnormalities In Hiv+ Brazilian Womenmentioning

confidence: 61%

Section: Prevalence Of Cervical Abnormalities In Hiv+ Brazilian Womenmentioning

confidence: 63%

Section: Molecular Prevalence Of Hpv and Genotypes In Hiv+ Women Accomentioning

confidence: 99%

See 1 more Smart Citation

HPV Infection and Cervical Abnormalities in HIV Positive Women in Different Regions of Brazil, a Middle-Income Country

Freitas

Suehiro²,

Consolaro³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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“…The fragments generated by digestion were visualized on an ultraviolet transilluminator. The samples were identified and typed according to the procedures by Melgaço et al (11) .…”

Section: Results Of Pcr and Restriction Fragment Length Polymorphismmentioning

confidence: 99%

“…Generic PCR allows detection of any HPV genotype using consensual primers MY09/MY11, which amplify a 450-pb fragment corresponding to L1 gene (11) . The reaction mixture was composed of 5 mL of 10´ PCR buffer (10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 10 mM NaCl), 1 mL dNTP (200 mmol dATP, dCTP, dGTP, dTTP), 3 mL MgCl 2 (50 mM), 1 mL primer (50 pM) to 0.25 mL DNA Taq polymerase, totaling 50 µL reaction with the addition of the sample.…”

Section: Generic Polymerase Chain Reaction With Consensual Primers Mymentioning

confidence: 99%

Human papillomavirus infection in multiple sites

Cordeiro¹,

Carestiato²,

Gouvêa³

et al. 2014

J bras doenças sex transm

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Introduction: Studies concerning human papillomavirus natural history have been focused on cervical infection and disease, but have scarcely described anal infections, especially in clinically health population. Hence, knowledge on HPV natural history is recently being investigated although viral maintenance in hosts is poorly understood. Detection on diverse, extragenital sites may add in the elucidation of infection-dissemination-reinfection cycle reported on the human genital tract. Besides that, it is evident the importance of an adequate screening of the viruses for appropriate diagnosis, mainly in the first steps of the neoplasia in order to provide a better prognosis. Our results pointed out HPV prevalence rates in genital lesions of 87% (80/92) and of 47.8% (44/92) for anal samples. We pointed out that this high prevalence is due to clinically detected lesions, that contributes to the risk of extragenital infections, mainly by host auto-inoculation. Despite the difference in samples size, we did not find statistical relevant differences related with genera (men 85.1% and women 92%; p>0.05), corroborating the idea of no tropism differences by sex. Nevertheless, for anal samples, statistically significant differences were found between men and women (68% of anal HPV infection in women against 40.3% in men (p=0.038). These results reveal that female anal infections are more frequent than in men, suggesting that infection by autoinoculation occurs and can render this site as an HPV reservoir, occasionally becoming clinical lesions. Hormonal profile, sexual behavior and differences among body sites can explain these differences here described. Our study is a preliminary evaluation of HPV infection in human multiple sites. Keywords: papillomavirus infections; polymerase chain reaction; anus. RESUMO Introdução:Muitos estudos sobre a história natural do papilomavírus humano (HPV) têm focado nas infecções cervicais, mas pouco tem se falado sobre infecções anais em pacientes assintomáticos. Assim, a história natural do HPV ainda está em construção e os mecanismos de manutenção dele no organismo hospedeiro são pouco compreendidos. Entendemos que a detecção do vírus em sítios extragenitais poderá ajudar na compreensão da cadeia de infecção-disseminação-reinfecção pelo HPV no trato genital humano. Além disso, é evidente a importância de um rastreio adequado do vírus diante de diagnósticos clínicos, principalmente no período inicial da lesão, provendo, assim, um melhor prognóstico. Nossos resultados apontaram uma prevalência da infecção por HPV em lesões genitais de 87% (80/92) e nas amostras anais tal prevalência foi de 47,8% (44/92). Ressaltamos que nossa prevalência foi alta, mas estudamos indivíduos com lesões genitais clinicamente detectadas, aumentando, assim, o risco de infecção em outros sítios e confirmando a ocorrência da presença do HPV no hospedeiro, provavelmente decorrente de autoinoculação. Apesar da diferença entre o número de indivíduos dos sexos feminino e masculino em nosso estudo, não enco...

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Simple and rapid human papillomavirus genotyping method by restriction fragment length polymorphism analysis with two restriction enzymes

Chen

Watanabe

Haruyama

et al. 2013

Journal of Medical Virology

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Cervical cancer, the third most common cancer that affects women worldwide, is caused by the human papillomavirus (HPV) and is treatable when detected at an early stage. To date, more than 100 different HPV types have been described, and the development of simple, low-cost, and accurate methods to distinguish HPV genotypes is highly warranted. In this study, an HPV genotyping assay based on polymerase chain reaction (PCR) was evaluated. This method involved the use of MY09/11 primers followed by restriction fragment length polymorphism (RFLP) analysis with the restriction enzymes HpyCH4V and NlaIII. Cervical specimens preserved using CytoRich Blue fluid were collected from 1,134 female volunteers for HPV detection, and 1,111 valid samples were amplified using PCR. The PCR method was sensitive enough to detect 25 copies of HPV18, and three copies of HPV16. Out of 202 PCR-positive samples, HPV genotypes were determined in 189 samples (93.6%) by this RFLP method. Results were then evaluated further by capillary sequencing method. Concordant results between the two tests were as high as 96.0%. Thirteen samples, which tested negative with RFLP, were verified as non-specific amplifications with PCR. In conclusion, this PCR-RFLP method using restriction enzymes HpyCH4V and NlaIII is simple, non-labor intensive, and is applicable for the inexpensive determination of HPV genotypes in clinical samples.

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Human papillomavirus genotypes distribution in cervical samples from women living with human immunodeficiency virus

Abstract: The analyzed sample represents an epidemiological source of uncommon infection. Due to high HPV prevalence, more frequent cytological screening and/or liberal colposcopic evaluations should be performed in HIV-positive patients.

Cited by 28 publications

References 40 publications

HPV Infection and Cervical Abnormalities in HIV Positive Women in Different Regions of Brazil, a Middle-Income Country

HPV Infection and Cervical Abnormalities in HIV Positive Women in Different Regions of Brazil, a Middle-Income Country

Human papillomavirus infection in multiple sites

Simple and rapid human papillomavirus genotyping method by restriction fragment length polymorphism analysis with two restriction enzymes

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