Human papillomavirus genotypes 68 and 58 are the most prevalent genotypes in women from quilombo communities in the state of Maranhão, Brazil

Batista, José Eduardo; Saddi, Vera Aparecida; Carvalho, Keila Patrícia Almeida de; Ribeiro, Andréa Alves; Segati, Kelly Deyse; Carneiro, Megmar Aparecida dos Santos; Monteiro, Sílvio Gomes; Nascimento, Maria do Desterro Soares Brandão; Rabelo-Santos, Sílvia Helena

doi:10.1016/j.ijid.2017.01.001

Cited by 12 publications

(13 citation statements)

References 22 publications

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“…In Northeastern Brazil, a number of papers have been published on HPV prevalence and type distribution among women with cytological and/or histological alterations [14,[30][31][32][33][34][35]. However, to the best of our knowledge, HPV prevalence among women with cervical lesions in the state of Sergipe, located in this region, has never been assessed.…”

Section: Discussionmentioning

confidence: 99%

“…Low-risk types 16 4.7 region of Brazil, HPV 68 was the most prevalent, but only women from quilombo communities with cytological abnormalities were assessed [35]. These results highlight the importance of assessing the prevalence of HPV types in other populations from this Brazillian region, which is associated with a high rate of cervical cancer incidence [2,38].…”

Section: Hpv Groupmentioning

confidence: 99%

“…In other regions of Brazil, HPV 58, 31, 33 and 56 were the second most prevalent HPV types among women with cervical abnormalities [5,37,[44][45][46][47]. In the Northeast region, HPV 58, 31, 74, 18 and 16 were the second most prevalent HPV types in abnormal cervical samples [14,[30][31][32][33][34][35]. Therefore, based on our observations, the state of Sergipe is the only state in Brazil that presented HPV 66 as the second most prevalent HPV type in women with cervical alterations.…”

Section: Hpv Groupmentioning

confidence: 99%

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Prevalence of human papillomavirus types associated with cervical lesions in Sergipe state, Northeastern Brazil: high frequency of a possibly carcinogenic type

et al. 2018

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Persistent infection with high-risk human papillomavirus (HPV) is the main cause of cervical cancer and the prevalence of HPV types varies depending on the geographic region. Therefore, this study assessed the prevalence of HPV types in women with cervical lesions from Sergipe state, Northeastern Brazil. A cross-sectional study was conducted in women with cervical lesions from March to December 2014. These lesions were investigated by PCR and HPV types were identified by DNA sequencing. 432 patients were included, of which 337 patients tested positive for HPV. Eighteen different HPV types were detected, and high-risk HPV types were detected in 69.2%. HPV 16 (63.4%) was the most prevalent HPV type found, followed by HPV 66 (4.6%), HPV 18 (1.6%) and HPV 45 (1.4%). These results highlight the importance of the high prevalence of HPV 66, which is a possibly carcinogenic virus type not covered by the available vaccines. The prevalence of HPV 16 was high in the studied population, reaffirming the importance of young vaccination. However, the high prevalence of HPV 66 found in this study shows the importance of monitoring the diversity of HPV types in different populations and geographic regions to better understand the impacts of current HPV vaccines.

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Section: Discussionmentioning

confidence: 99%

Section: Hpv Groupmentioning

confidence: 99%

Section: Hpv Groupmentioning

confidence: 99%

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Prevalence of human papillomavirus types associated with cervical lesions in Sergipe state, Northeastern Brazil: high frequency of a possibly carcinogenic type

et al. 2018

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“…This is consistent with another study in the same region of Kenya where a high HPV68 prevalence among HIV-infected women was found 33 . A study conducted in an isolated rural community in Brazil reported HPV68 as the most prevalent genotype, however it was not present in women with cytological abnormalities 45 . The epidemiological importance of HPV68 needs to be further evaluated, especially as assay-related variation in HPV68 detection has been reported 46 .…”

Section: Hpv Genotypesmentioning

confidence: 95%

Novel Concepts in Cervical Cancer Screening. A Comparison of VIA, HPV DNA Test and p16ink4a/Ki-67 Dual-Stain Cytology in Western Kenya.

Orang’o

Were

Rode

et al. 2020

Preprint

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Abstract Background: Screening of unvaccinated women remains essential to mitigate the high morbidity/mortality of cervical cancer. Here, we compared visual inspection with acetic acid (VIA), recommended by WHO as the most cost-effective screening approach in LMICs, with HPV-based screening, and usage of p16INK4a/Ki-67 dual stain cytology.Methods: We prospectively enrolled women participating in a VIA-based cervical cancer screening program in two peri-urban health centers of Kenya. Consenting women had a VIA examination preceded by collection of a liquid-based cytology sample from the cervix stored in PreservCyt medium (Hologic®). Analysis of all samples included a hrHPV DNA test and evaluation of a p16 INK4a /Ki-67 (CINtecPLUS®) dual-stained slide that was prepared using the ThinPrep® 2000 Processor and evaluated by a pathologist trained in the methodology.Results: In 701 of a total of 800 women aged 18-64 years, all three investigations were performed and data could be analyzed. The HPV, VIA and dual-stain cytology positivity were 33%, 7%, and 2% respectively. The HPV positivity rate of VIA positive cases was 32%. The five most common HPV types were HPV16, 52, 68, 58 and 35. The OR among HIV infected women of an HPV infection, VIA positivity and positive dual stain cytology were 2.6 (95%CI 1.5-4.3), 1.9 (95%CI 0.89-4.4) and 3.4 (95%CI 1.07-10.9) respectively. The sensitivity of VIA to detect a p16INK4a/Ki-67 positive transforming infection was 13% (95%CI 2-38). Conclusions: Primary HPV testing appears feasible and should be considered as a primary screening test also in LMICs. The poor sensitivity of VIA renders it unsuitable as a triage test for HPV positive women. The utility of p16ink4a/Ki-67 dual-stain cytology as a triage test for HPV positive women in LMICs should be further studied.

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“…The second batch (N = 383) was tested at AML laboratory, Antwerp, Belgium, using the TaqMan-based qPCR assay (RIATOL, Sonic Healthcare Benelux, Antwerp, Belgium) which detects 17 HPV genotypes and β -globin in seven multiplex reactions. These HPV types include all 12 high-risk types (HPV16, 18,31,33,35,39,45,51,52,56,58,59), three probably high-risk types (HPV53, 66 and 68), one low-risk type (HPV6) and one undetermined risk type (HPV67) [32]. All HC2 positive and a subset of HC2 negatives samples were retested using the TaqMan-based qPCR assay.…”

Section: Laboratory Testingmentioning

confidence: 99%

Novel concepts in cervical cancer screening: a comparison of VIA, HPV DNA test and p16INK4a/Ki-67 dual stain cytology in Western Kenya

Orang’o

Were

Rode

et al. 2020

Infect Agents Cancer

View full text Add to dashboard Cite

Background Screening of unvaccinated women remains essential to mitigate the high morbidity/mortality of cervical cancer. Here, we compared visual inspection with acetic acid (VIA), recommended by WHO as the most cost-effective screening approach in LMICs, with HPV-based screening, and usage of p16INK4a/Ki-67 dual stain cytology. Methods We prospectively enrolled women participating in a VIA-based cervical cancer screening program in two peri-urban health centers of Kenya. Consenting women had a VIA examination preceded by collection of a liquid-based cytology sample from the cervix stored in PreservCyt medium (Hologic®). Analysis of all samples included a hrHPV DNA test and evaluation of a p16INK4a /Ki-67 (CINtecPLUS®) dual stained slide that was prepared using the ThinPrep® 2000 Processor and evaluated by a pathologist trained in the methodology. Results In 701 of a total of 800 women aged 18–64 years, all three investigations were performed and data could be analyzed. The HPV, VIA and dual stain cytology positivity were 33%, 7%, and 2% respectively. The HPV positivity rate of VIA positive cases was 32%. The five most common HPV types were HPV16, 52, 68, 58 and 35. The OR among HIV infected women of an HPV infection, VIA positivity and positive dual stain cytology were 2.6 (95%CI 1.5–4.3), 1.9 (95%CI 0.89–4.4) and 3.4 (95%CI 1.07–10.9) respectively. The sensitivity of VIA to detect a p16INK4a/Ki-67 positive transforming infection was 13% (95%CI 2–38). Conclusions Primary HPV testing appears feasible and should be considered as a primary screening test also in LMICs. The poor sensitivity of VIA renders it unsuitable as a triage test for HPV positive women. The utility of p16INK4a/Ki-67 dual stain cytology as a triage test for HPV positive women in LMICs should be further studied.

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Human papillomavirus genotypes 68 and 58 are the most prevalent genotypes in women from quilombo communities in the state of Maranhão, Brazil

Abstract: A distinct profile of high-risk HPV genotypes was detected, with predominance of types 68 and 58. It is possible that the results of the present study are due to specific characteristics of the population, which is geographically isolated and maintains conservative sexual habits.

Cited by 12 publications

References 22 publications

Prevalence of human papillomavirus types associated with cervical lesions in Sergipe state, Northeastern Brazil: high frequency of a possibly carcinogenic type

Prevalence of human papillomavirus types associated with cervical lesions in Sergipe state, Northeastern Brazil: high frequency of a possibly carcinogenic type

Novel Concepts in Cervical Cancer Screening. A Comparison of VIA, HPV DNA Test and p16ink4a/Ki-67 Dual-Stain Cytology in Western Kenya.

Novel concepts in cervical cancer screening: a comparison of VIA, HPV DNA test and p16INK4a/Ki-67 dual stain cytology in Western Kenya

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