Human papillomavirus genotype detection and viral load in paired genital and urine samples from both females and males

Bissett, Sara L.; Howell-Jones, Rebecca; Swift, Craig; Silva, Natasha De; Biscornet, Leon; Parry, John; Saunders, Nicholas A.; Nathan, Mayura; Soldan, Kate; Szarewski, Anne; Cuzick, Jack; Beddows, Simon

doi:10.1002/jmv.22167

Cited by 41 publications

(55 citation statements)

References 45 publications

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“…In our study, the agreement between any HPV type detection in urine and cervical samples was 84.00% with a Cohen's kappa coefficient of 0.60, similar to that reported by Cuschieri and colleagues. In relation to concordance for LR-HPV and HR-HPV types included in tetravalent vaccine, in our study, concordance was substantial for both, k ¼ 0.70 (95% CI, 0.66-0.74) for HPV-6 and -11 and k ¼ 0.76 (95% CI, 0.78-0.84) for HPV-16 and -18 (31,37,41,(43)(44)(45).…”

Section: Discussionmentioning

confidence: 52%

Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Cómbita

Gheit

González

et al. 2016

Cancer Prevention Research

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Urine sampling for HPV DNA detection has been proposed as an effective method for monitoring the impact of HPV vaccination programs; however, conflicting results have been reported. The goal of this study was to evaluate the performance of optimized urine HPV DNA testing in women aged 19 to 25 years. Optimization process included the use of first void urine, immediate mixing of urine with DNA preservative, and the concentration of all HPV DNA, including cell-free DNA fragments. Urine and cervical samples were collected from 535 young women attending cervical screening at health centers from two Colombian cities. HPV DNA detection and genotyping was performed using an HPV type-specific multiplex genotyping assay, which combines multiplex polymerase chain reaction with bead-based Luminex technology. Concordance between HPV DNA detection in urine and cervical samples was determined using kappa statistics and McNemar tests. The accuracy of HPV DNA testing in urine samples was evaluated measuring sensitivity and specificity using as reference the results obtained from cervical samples. Statistical analysis was performed using STATA11.2 software. The findings revealed an overall HPV prevalence of 60.00% in cervical samples and 64.72% in urine samples, HPV-16 being the most frequent HPV type detected in both specimens. Moreover, our results indicate that detection of HPV DNA in first void urine provides similar results to those obtained with cervical samples and can be used to monitor HPV vaccination trials and programs as evidenced by the substantial concordance found for the detection of the four vaccine types.Cancer Prev Res; 9(9); 766-71. Ó2016 AACR.

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Section: Discussionmentioning

confidence: 52%

Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Cómbita

Gheit

González

et al. 2016

Cancer Prevention Research

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“…It was different from a previous survey in Thai women, which reported HPV16 as the most common genotype identified (17.9%) (Chansaenroj et al, 2010). HPV 16 remained the most prevalent HPV genotype in Thailand as well as in many other countries (Onuki et al, 2009;Bissett et al, 2011;Munoz et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Detection Sensitivity for Human Papillomavirus between Self-collected Vaginal Swabs and Physician-collected Cervical Swabs by Electrochemical DNA Chip

Nilyanimit¹,

Wanlapakorn²,

Niruthisard³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Background: Human papillomavirus (HPV) DNA testing is an effective method to screen for precancerous changes in the cervix. Samples from self-collection rather than Pap smear can potentially be used to test for HPV as they are more acceptable and preferred for use in certain settings. The objective of this study was to compare HPV DNA testing from self-collected vaginal swabs and physician-collected cervical swabs. Materials and Methods: A total of 101 self-collected vaginal and physician-collected cervical swabs of known cytology from Thai women were tested by electrochemical DNA chip assay. The specimens were divided into 4 groups: 29 with normal cytology, 14 with atypical squamous cells of undetermined significance (ASCUS), 48 with lowgrade squamous intraepithelial lesion (LSIL), and 10 with high-grade squamous intraepithelial lesion (HSIL). Results: Positive detection rates of HPV from self-collected swabs were similar to those from physician-collected swabs. Among specimens with abnormal cytology, HPV was found in 50% of self-collected swabs and 47.2% of physician-collected swabs. In specimens with normal cytology, 17.2% of self-collected swabs and 24.1% of physician-collected swabs were positive for HPV. Concordance was relatively high between results from selfcollected and physician-collected samples. The most common HPV genotype detected was HPV 51. Conclusions: HPV DNA testing using self-collected swabs is a feasible alternative to encourage and increase screening for cervical cancer in a population who might otherwise avoid this important preventive examination due to embarrassment, discomfort, and anxiety.

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“…This was different from a previous survey in Thai women which reported HPV16 as the most common genotype identified (17.9%), followed by HPV 90 (16.6%) and HPV 71 (10.3%) (Chansaenroj et al, 2010). Regardless of differences in collection methods and PCR techniques, HPV 16 is still the most prevalent HPV genotype in Thailand as well as in many other countries (Onuki et al, 2009;Bissett et al, 2011;Munoz et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Several other studies have compared HPV sampling rates between urine and cervical samples in order to gauge the ability to detect HPV prevalence independently of cervical cytology (Sellors et al, 2000;Brinkman et al, 2002;Daponte et al, 2006;Bissett et al, 2011;Munoz et al, 2013). Daponte et al (2006) suggested that sensitivity of urine testing for HPV 16 and 18 was higher in cervical cancer (88.8%) than in high grade and low grade lesions (76.5 and 45.5%, respectively) (Daponte et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…However, urine samples have some limitations as it doesn't directly come from the original sites of the disease. In addition, some reports (Song et al, 2007;Bissett et al, 2011) have demonstrated a lower sensitivity of HPV detection in urine compared with cervical swab samples, although other reports (Daponte et al, 2006;Gupta et al, 2006) have shown comparable sensitivity.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Human Papillomavirus in Male and Female Urine by Electrochemical DNA Chip and PCR Sequencing

Nilyanimit

Wanlapakorn²,

Niruthisard³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Cervical cancer is the second most common cancer in Thai women after breast cancer. Currently, the Papanicolaou (Pap) smear is the recommended procedure for cervical cancer screening in Thailand, but only a relatively small percentage of women follow this screening program. An alternative method to detect HPV genotypes associated with cervical cancer is self-sampling of urine, which is a more widely accepted method. Our study aimed to evaluate the prevalence of HPV in Thai women using urine and cervical swabs and prevalence of HPV in Thai men using urine samples. Materials and Methods: Tumorigenic HPV detection was accomplished by electrochemical DNA chip and PCR/direct sequencing. In addition to HPV prevalence, we report the concordance between different methods and sample types. One-hundred and sixteen women and 100 men were recruited. Histological examination revealed normal cytology in 52 women, atypical squamous cells of undetermined significance (ASCUS) in 9, low-grade squamous intraepithelial lesions (LSIL) in 24, and high-grade squamous intraepithelial lesions (HSIL) in 31. One-hundred men were classified as heterosexuals (n=45) and homosexuals (n=55). Results: The most prevalent HPV genotype in our study was HPV16. The HPV detection rate was generally lower in urine samples compared with cervical samples. Overall, there was good agreement for the detection of carcinogenic HPV from female cervical samples between the DNA chip and PCR/ sequencing, with 88.8% total agreement and a kappa value of 0.76. In male urine samples, the level of agreement was higher in heterosexuals compared with homosexuals. Conclusions: Further improvement is required to increase an overall yield of HPV DNA detection in urine samples before clinical application of a urine-based HPV screening program. The electrochemical DNA chip test is a promising technique for carcinogenic HPV detection.

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Human papillomavirus genotype detection and viral load in paired genital and urine samples from both females and males

Cited by 41 publications

References 45 publications

Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Comparison of Detection Sensitivity for Human Papillomavirus between Self-collected Vaginal Swabs and Physician-collected Cervical Swabs by Electrochemical DNA Chip

Detection of Human Papillomavirus in Male and Female Urine by Electrochemical DNA Chip and PCR Sequencing

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