Human papillomavirus genomics: Understanding carcinogenicity

Nelson, Chase W.; Mirabello, Lisa

doi:10.1016/j.tvr.2023.200258

Cited by 32 publications

(28 citation statements)

References 173 publications

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“…Human papilloma virus (HPV) infections have a high incidence in the general population, with genital HPV infection being the most prevalent sexually transmitted infection in the world [ 1 ]. It is estimated that more than 75% of sexually active women contract it in their lifetime [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…Despite these findings, and the fact that these types of cancer have a prognosis closely related to diagnostic timing, currently, oral tumor screening is not carried out [ 24 ]. This is because early diagnosis is considered nearly impossible due to the frequent presence of HPV, even in the absence of visible lesions in the oral cavity [ 1 ]. In light of this, the main objective of our study was to evaluate the potential connection between the presence of HPV in both the oral and genital mucous membranes in women with HPV lesions at the lower genital tract.…”

Section: Introductionmentioning

confidence: 99%

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Analogies between HPV Behavior in Oral and Vaginal Cavity: Narrative Review on the Current Evidence in the Literature

Dellino,

Pinto,

D’Amato

et al. 2024

JCM

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Human genital papilloma virus infection is the most prevalent sexually transmitted infection in the world. It is estimated that more than 75% of sexually active women contract this infection in their lifetime. In 80% of young women, there is the clearance of the virus within 18–24 months. In developed countries, oral squamous cell carcinoma (OSCC) is now the most frequent human papilloma virus (HPV)-related cancer, having surpassed cervical cancer, and it is predicted that by 2030 most squamous cell carcinomas will be the HPV-related rather than non-HPV-related form. However, there are currently no screening programs for oral cavity infection. While the natural history of HPV infection in the cervix is well known, in the oropharynx, it is not entirely clear. Furthermore, the prevalence of HPV in the oropharynx is unknown. Published studies have found wide-ranging prevalence estimates of 2.6% to 50%. There are also conflicting results regarding the percentage of women presenting the same type of HPV at two mucosal sites, ranging from 0 to 60%. Additionally, the question arises as to whether oral infection can develop from genital HPV infection, through oral and genital contact or by self-inoculation, or whether it should be considered an independent event. However, there is still no consensus on these topics, nor on the relationship between genital and oral HPV infections. Therefore, this literature review aims to evaluate whether there is evidence of a connection between oral and cervical HPV, while also endorsing the usefulness of the screening of oral infection in patients with high-risk cervical HPV as a means of facilitating the diagnosis and early management of HPV-related oral lesions. Finally, this review emphasizes the recommendation for the use of the HPV vaccines in primary prevention in the male and female population as the most effective means of successfully counteracting the increasing incidence of OSCC to date.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analogies between HPV Behavior in Oral and Vaginal Cavity: Narrative Review on the Current Evidence in the Literature

Dellino,

Pinto,

D’Amato

et al. 2024

JCM

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“…E6 binds and inactivates P53, thereby inhibiting apoptosis. The viral E7 binds to pRb and separates E2F from pRb, leading to cell cycle progression [ 15 ]. This triggers p16 to exert its function by inhibiting CDK4-mediated phosphorylation of pRb.…”

Section: Introductionmentioning

confidence: 99%

HPV-Related Oropharyngeal Cancer in Southern Thailand: Proportion Trend and Survival Outcome

Saiya,

Jantharapattana,

Dechaphunkul

et al. 2024

Asian Pac J Cancer Prev

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Background: Persistent high-risk human papillomavirus (HPV) infection is one of the major etiologies of oropharyngeal squamous cell carcinoma (OPSCC). This study aimed to determine the proportion, temporal trend, and prognostic significance of HPV-related OPSCC in Thai patients. Methods: The study included patients with OPSCC who were treated at Songklanagarind Hospital (Songkhla, Southern Thailand) from 2009 to 2020. HPV status was screened by p16 expression using immunohistochemistry and confirmed by real-time polymerase chain reaction. Cox regression was used to determine prognostic significance. Results: The overall proportion of HPV+ OPSCC was 15.3% (95% confidence interval [CI]: 12.1–18.5) with a slightly increased proportion from 10.6% in 2009–2010 to 16.5% (2019–2020) (P for trend = 0.166). Among the HPV+ cases, HPV16 was detected in 65.3%, HPV18 in 34.7%, and other high-risk HPV types in 24%. Patients with P16+ or HPV+ OPSCC had significantly better overall survival (hazard ratio [HR]: 0.63, 95% CI: 0.45–0.90 and HR: 0.63, 95% CI: 0.45–0.88, respectively). Conclusion: Thai patients in the southern region have a low proportion of HPV-related OPSCC with an increasing trend. Both P16 expression and HPV DNA status are strong independent prognostic factors of OPSCC.

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“…Human papillomavirus (HPV) is the major underlying cause of cervical cancer. Approximately 604,000 new instances of cervical cancer are estimated to occur worldwide each year, resulting in an approximate annual mortality rate of 342,000 [1,2]. In Indonesia, cervical cancer ranks as the ninth most common cause of death and has been categorized as the third deadliest form of cancer [1,3].…”

Section: Introductionmentioning

confidence: 99%

“…The early region is comprised of six open reading frames (ORFs): E1, E2, and E4, which are involved in supporting viral function, and E6, E7, and E5, which are responsible for encoding viral oncoproteins. The late region (L) comprises two open reading frames (ORFs), L1 and L2, which encode essential structural capsid proteins required for viral replication [2,7].…”

Section: Introductionmentioning

confidence: 99%

Expression of human papillomavirus genotype 52 pseudovirus in HEK-293T cells

Kusumawati,

Septisetyani,

Triratna

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Human papillomavirus (HPV) is the predominant cause of cervical cancer globally. HPV genotype 52 is categorized as a high-risk type and holds the highest prevalence in Indonesia (23.2 %). The HPV pseudovirus can be used to test the immunogenicity of HPV vaccine candidates and evaluate the neutralization efficiency of the antibodies against the virus. The aim of this study was to express the HPV52 L1 and L2 capsid protein into pseudovirion containing the GFP reporter plasmid (pfwB) in HEK-293T cells. HPV52 L1 and L2 coding plasmid (pVITRO52) along with pfwB were amplified in E. coli. The plasmids were extracted and co-transfected using polyethyleneimine (PEI)-based transfection method into the HEK-293T cells. The expression of HPV52 pseudoviruses were confirmed by fluorescence microscopy and western blot method. Co-transfection of HPV52 L1 and L2 coding plasmid (pVITRO52) along with pfWB into the HEK-293T cells was successfully carried out. The co–transfected HEK-293T cells showed fluorescence. The western blot assay using HPV52 L1 protein primary antibody showed a band around ∼55 kDa. In the future, the results of this study will be used to evaluate the immunogenicity and neutralization assay of HPV vaccine candidates.

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Human papillomavirus genomics: Understanding carcinogenicity

Cited by 32 publications

References 173 publications

Analogies between HPV Behavior in Oral and Vaginal Cavity: Narrative Review on the Current Evidence in the Literature

Analogies between HPV Behavior in Oral and Vaginal Cavity: Narrative Review on the Current Evidence in the Literature

HPV-Related Oropharyngeal Cancer in Southern Thailand: Proportion Trend and Survival Outcome

Expression of human papillomavirus genotype 52 pseudovirus in HEK-293T cells

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