Human papillomavirus DNA in cervical intraepithelial neoplasia detected by in situ hybridisation

Cardillo, Marcel; Marino, Raffaele; Possi, Vincenzo

doi:10.1016/0277-5379(91)90486-w

Cited by 7 publications

(3 citation statements)

References 18 publications

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“…(B) Dot blot analysis of some biopsies whose PCR-positive samples are shown in panel A. Hybridization signals can be seen at positions Al, A3, C7, and D10. 29% by ISH is similar to that reported in other studies in which biotinylated probes were used (10). The frequency of HPV DNA detection is known to increase with the use of radiolabeled probes (38).…”

Section: Discussionsupporting

confidence: 85%

Detection of human papillomavirus 16 and 18 DNA in epithelial lesions of the lower genital tract by in situ hybridization and polymerase chain reaction: cervical scrapes are not substitutes for biopsies

et al. 1993

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Human papillomavirus (HPV) types 16 and 18 in 66 women with histologically documented lesions of the genital tract and 64 control cohorts were investigated. The efficacies of in situ hybridization and polymerase chain reaction (PCR) in detecting HPV 16 and 18 DNA were analyzed. In order to assess the usefulness of replacing biopsies with cervical scrapes, the two samples were compared by PCR. The prevalence rates of HPV infection by PCR were 59.1 and 10.9% in patients and controls, respectively. PCR was three times more sensitive than in situ hybridization (52.6 versus 17.8%). However, the need to improve PCR sensitivity by subsequent dot blot hybridization reduced one of the main advantages of PCR, i.e., expeditious diagnosis. Cervical scrapes were less sensitive than biopsies (13.6 versus 53%), although with four (6.1%) patients with intraepithelial neoplasias, HPV DNA was identified only by means of cervical scraping. We conclude that obtaining biopsy specimens and cervical scraping are complementary sampling procedures. Human papillomaviruses (HPVs) are members of the Papovaviridae family and contain circular double-stranded DNA approximately 8 kb in length (2). The application of DNA hybridization techniques led to the characterization of distinct HPV types (15, 19). More than 66 different genotypes have been identified (45), and approximately 18 of them have been associated with neoplastic lesions of the anogenital mucosa and skin (26). The association of particular types with genital cancer is now well documented (18, 54). On this basis, HPVs have been categorized into low-risk (e.g., HPV 6 and 11) and high-risk (e.g., HPV 16, 18, 31, 33, and 35) virus types reflecting their oncogenic potential. HPV 6 and 11 frequently coexist with cervical condylomata and minor dysplasias (4), while HPV 16 and 18 have been found in high-grade premalignancies and invasive cancers (26, 41). Important differences in the physical states of HPV DNA have been demonstrated with benign and malignant lesions.

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Section: Discussionsupporting

confidence: 85%

Detection of human papillomavirus 16 and 18 DNA in epithelial lesions of the lower genital tract by in situ hybridization and polymerase chain reaction: cervical scrapes are not substitutes for biopsies

et al. 1993

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“…Με την μέθοδο του υβριδισμού λοιπόν, συγκεντρώνουμε τα προς εξέταση κύτταρα σε ένα φίλτρο και διαχωρίζουμε τις διπλές λωρίδες DNA των χρωμοσωμάτων και κυττάρων. Τμήματα αυτών των λωρίδων DNA (εφ'όσον τα κύτταρα είναι μολυσμένα) ανήκουν στον ιό (HPV ) (12,17,19,20,21,53,82).…”

Section: υβριδισμόςunclassified

Επιδημιολογικη Μελετη Κυτταρολογικων Επιχρισματων Κατα Παπανικολαου Σε Ελληνικοπληθυσμο Υπαιθρου

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Section: υβριδισμόςunclassified

Κλινικά Και Εργαστηριακά Αποτελέσματα Εξέτασης Κολπικού Επιχρίσματος Κατά Παπανικολάου

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Human papillomavirus DNA in cervical intraepithelial neoplasia detected by in situ hybridisation

Cited by 7 publications

References 18 publications

Detection of human papillomavirus 16 and 18 DNA in epithelial lesions of the lower genital tract by in situ hybridization and polymerase chain reaction: cervical scrapes are not substitutes for biopsies

Detection of human papillomavirus 16 and 18 DNA in epithelial lesions of the lower genital tract by in situ hybridization and polymerase chain reaction: cervical scrapes are not substitutes for biopsies

Επιδημιολογικη Μελετη Κυτταρολογικων Επιχρισματων Κατα Παπανικολαου Σε Ελληνικοπληθυσμο Υπαιθρου

Κλινικά Και Εργαστηριακά Αποτελέσματα Εξέτασης Κολπικού Επιχρίσματος Κατά Παπανικολάου

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