Human papillomavirus detection in histologic samples of multifocal epithelial hyperplasia: a novel demographic presentation

Khanal, Sujita; Cole, Eric T.; Joh, Joongho; Ghim, Shin-je; Jenson, A. Bennett; Shesh, N.; Trainor, Patrick J.; Shumway, Brian S.

doi:10.1016/j.oooo.2015.07.035

Cited by 12 publications

(6 citation statements)

References 46 publications

(58 reference statements)

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“…The possibility that some papillomas could have a non-viral cause is suggested by the inability to detect HPV DNA in a large proportion of lesions. In contrast, nearly all cases of oral condyloma acuminatum contain identifiable HPV ( 1 , 4 ) ( 5 ) and 86% of multifocal epithelial hyperplasia samples are HPV-positive ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

Detection of human papillomavirus DNA in formalin-fixed, paraffin-embedded squamous papillomas of the oral cavity

Daigrepont¹,

Cameron²,

Wright³

et al. 2018

J Clin Exp Dent

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BackgroundSquamous papillomas are exophytic proliferations of surface oral epithelium. Human papillomavirus (HPV) infection is widely accepted as the etiology of squamous papillomas however the virus cannot be detected in a significant percentage of lesions.Material and MethodsUsing polymerase chain reaction (PCR), we tested 35 formalin-fixed paraffin-embedded (FFPE) squamous papillomas for the presence of HPV DNA.ResultsSix papillomas (17%) tested positive for HPV DNA; four contained HPV-6 and two contained HPV-11. Given that β–globin DNA was only identified in half of the samples, DNA degradation appears to have significantly impacted the results.ConclusionsThe results likely represent an underestimation of the true number of HPV-positive specimens in our study. Potential explanations for HPV-negative squamous papillomas include transient HPV infection, failure of the experiment to detect HPV if present, or the possibility that some lesions may not result from HPV infection. Key words:HPV, PCR, FFPE, papilloma, oral.

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Section: Introductionmentioning

confidence: 99%

Detection of human papillomavirus DNA in formalin-fixed, paraffin-embedded squamous papillomas of the oral cavity

Daigrepont¹,

Cameron²,

Wright³

et al. 2018

J Clin Exp Dent

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“…Focal epithelial hyperplasia and oral condyloma acuminata have similar clinical and histopathological characteristics. Both are caused by low-risk HPVs, with condyloma being more often associated with HPV-6 and HPV-11, although several other types (including HPV-32 and more rarely high-risk HPVs) have been described in these lesions 4,8,9 .…”

Section: Discussionmentioning

confidence: 99%

Unusual presentation of a disseminated oral HPV infection after combined antiretroviral therapy initiation

Vale

Ferracini

Bueno

et al. 2019

Rev. Inst. Med. trop. S. Paulo

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HPV clinical manifestations have their characteristics modified by the use of combined antiretroviral therapy (cART), although its incidence is unaffected by cART. We report an unusual presentation of oral HPV infection and discuss an effective treatment for disseminated HPV lesions. A 52-year-old male of Asian-origin, HIV-seropositive, presented with extensive nodular lesions throughout the oral mucosa extending to the oropharyngeal region. Biopsy followed by histopathological examination and HPV genotyping were performed. The treatment was initiated with topical application of podophyllin and trichloroacetic acid. HPV lesions in oral mucosa are generally easy to handle. Extensive lesions can make it difficult to choose an effective treatment that meets the patient’s particularities and medication availability.

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“…FFPE samples were deparaffinized with xylene and washed before DNA extraction. HPV detection and genotyping were performed with 16E7 primers ( Supplementary Table 1 ), and β-globin was used as an internal control, as previously described [ 61 ]. HPV16-positive cases (18/38 for hgOED and 16/50 for HNSCC) were then used in HPV DNA integration and methylation studies.…”

Section: Methodsmentioning

confidence: 99%

Viral DNA integration and methylation of human papillomavirus type 16 in high-grade oral epithelial dysplasia and head and neck squamous cell carcinoma

et al. 2018

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This study evaluated the integration and methlyation of human papillomavirus type 16 (HPV16) in head and neck squamous cell carcinoma (HNSCC) and its oral precursor, high-grade oral epithelial dysplasia (hgOED). Archival samples of HPV16-positive hgOED (N = 19) and HNSCC (N = 15) were evaluated, along with three HNSCC (UMSCC-1, -47 and -104) and two cervical cancer (SiHa and CaSki) cell lines. HgOED cases were stratified into three groups with increasing degrees of cytologic changes (mitosis, karyorrhexis and apoptosis). The viral load was higher and the E2/E6 ratio lower (indicating a greater tendency toward viral integration) in group 3 than in groups 1 or 2 (p = 0.002, 0.03). Methylation was not observed in hgOED cases and occurred variably in only three HNSCC cases (26.67%, 60.0% and 93.3%). In HNSCC cell lines, lower E7 expression correlated with higher levels of methylation. HgOED with increased cytologic change, now termed HPV-associated oral epithelial dysplasia (HPV-OED), exhibited an increased viral load and a tendency toward DNA integration, suggesting a potentially increased risk for malignant transformation. More detailed characterization and clinical follow-up of HPV-OED patients is needed to determine whether HPV-OED is a true precursor to HPV-associated HNSCC and to clarify the involvement of HPV in HNSCC carcinogenesis.

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Human papillomavirus detection in histologic samples of multifocal epithelial hyperplasia: a novel demographic presentation

Cited by 12 publications

References 46 publications

Detection of human papillomavirus DNA in formalin-fixed, paraffin-embedded squamous papillomas of the oral cavity

Detection of human papillomavirus DNA in formalin-fixed, paraffin-embedded squamous papillomas of the oral cavity

Unusual presentation of a disseminated oral HPV infection after combined antiretroviral therapy initiation

Viral DNA integration and methylation of human papillomavirus type 16 in high-grade oral epithelial dysplasia and head and neck squamous cell carcinoma

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