Human papillomavirus-associated squamous cell carcinoma of the larynx or hypopharynx: Clinical outcomes and implications for laryngeal preservation

Hughes, Ryan T.; Beuerlein, William J.; O’Neill, Stacey S.; Porosnicu, Mercedes; Lycan, Thomas; Waltonen, Joshua D.; Frizzell, Bart; Greven, Kathryn M.

doi:10.1016/j.oraloncology.2019.09.008

Cited by 25 publications

(28 citation statements)

References 40 publications

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“…Also, another study by Kreimer et al has shown that the rate of infection with HPV in laryngeal cancer tissue was 24%, which is in agreement with our results, but also showing that a 19% HPV infection rate was seen in normal laryngeal mucosa as well [27]. In accordance with other studies, our results have shown 18.2% HPV-driven laryngeal cancer [26,29], compared to studies that showed a lower association [30,31].…”

Section: Discussionsupporting

confidence: 93%

“…HPV detection and genotype identification were carried out using Real-time PCR (RT-PCR) thermal cycler (Bioer, China). REALQUALITY RQ-Multi HPV detection is an in vitro diagnostic kit (AB ANALITICA, Italy) which is capable of identifying 28 HPV types including 14 high-risk (16,18,31,33,35,39,45,51,52,56,58,59,66,68), 6 potential high-risk (26,53,67,70,73,82), and 8 low-risk (6,11,40,42,43,44,55,83) genotypes [14]. The kit amplifies a fragment in the region of E6 and E7 in the HPV genome and detects the different genotypes as a pool except for HPV 16 and HPV 18 which are detected individually.…”

Section: Hpv Detection and Genotypingmentioning

confidence: 99%

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Prevalence of Human Papillomavirus Associated with Head and Neck Squamous Cell Carcinoma in Jordanian Patients

Khasawneh¹,

Himsawi²,

Abu-Raideh³

et al. 2020

TOMICROJ

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Background: In addition to smoking and alcohol consumption, human papillomavirus (HPV) is a leading etiology for Head and Neck Squamous Cell Carcinoma (HNSCC). However, this causal association is still understudied in Middle Eastern populations. Objective: The aim of this study was to determine the prevalence of HPV-associated infection in the Jordanian HNSCC patients and the associated HPV genotypes. Methods: Formalin-Fixed Paraffin-Embedded (FFPE) squamous cell carcinoma samples of the head and neck were collected from two referral centers in Amman, Jordan to determine the existence of HPV DNA. After DNA extraction HPV infection and genotyping were identified using real-time PCR. Results: HPV DNA was detected in 19 out of 61 (31.1%) HNSCC samples. Despite screening for 28 different genotypes, HPV 16 was the only genotype identified in all examined samples. Most HPV-positive samples were obtained from the oropharynx (41.7%), oral cavity (37%), and larynx (18.2%). No significant association between HPV 16 genotype and age, sex, tobacco use, anatomical location, or tumor grade was noticed. Conclusion: This study reported a high association between HPV 16 genotype and HNSCC in Jordanian patients. These data should facilitate the implementation of appropriate HPV awareness campaigns, and activate selective prophylactic measures against HPV infection.

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Section: Discussionsupporting

confidence: 93%

Section: Hpv Detection and Genotypingmentioning

confidence: 99%

Prevalence of Human Papillomavirus Associated with Head and Neck Squamous Cell Carcinoma in Jordanian Patients

Khasawneh¹,

Himsawi²,

Abu-Raideh³

et al. 2020

TOMICROJ

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“…Chung et al 12 16,17,27 were combined with other subsites and/or were limited to patients with locally advanced disease, 28 or included patients with metastatic disease and those who did not receive appropriate therapies. 17 In comparison, our analysis of 18,19,[29][30][31] though most were limited by a small sample size…”

Section: Discussionmentioning

confidence: 95%

Prognostic role of human papilloma virus status in hypopharyngeal squamous cell carcinoma

Marshall

Kao

Bakst

et al. 2020

Laryngoscope Investig Oto

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Objective: Although the prognostic role of human papilloma virus (HPV) status in oropharyngeal head and neck squamous cell carcinoma (SCC) is well established, growing evidence shows that there may be a prognostic role for HPV status in hypopharyngeal SCC. The objective of this study was to determine the prognostic role of HPV status in hypopharyngeal SCC. Methods: We performed a retrospective, population-based analysis of 1934 adult patients with HNSCC diagnosed between 2010-2016 and treated with a combination of surgery and/or radiotherapy, with or without chemotherapy, and a subset of 641 patients with hypopharyngeal SCC and known HPV status included in the Surveillance, Epidemiology, and End Results (SEER) Head and Neck with HPV Status Database. Patient data were used to determine the adjusted 2-year cancer-specific survival (CSS) and overall survival (OS) for the entire cohort and the specific subgroup of hypopharyngeal cancer patients with known HPV status. Results: Of the 1934 hypopharynx SCC cases, HPV status was unknown in 1294 (66.9%), and 167 (8.6%) were HPV positive; among hypopharynx cases with known HPV status, 21.6% were HPV positive. In models adjusting for sex, age, race/ethnicity, marital status and stage, patients with HPV-positive hypopharyngeal tumors had

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“…Indeed, comparing all SCC from diverse anatomical sites identifies HPV positive tumors (principally from the head and neck and cervix) as a clear genetic subclass of SCC 1 . However, recent data does suggest that HPV infection in the larynx, hypopharynx, or oral cavity, does not change overall prognosis 9,10 , while in the oropharynx HPV has prognostic significance 8 , again supporting the idea that anatomical sub-site influences outcomes. For HPV negative tumors further sub-classification can be based on copy number alterations (CNA) or presence of specific mutations and epigenetic variation 11–14 .…”

Section: Introductionmentioning

confidence: 98%

Mutation signature analysis identifies increased mutation caused by tobacco smoke associated DNA adducts in larynx squamous cell carcinoma compared with oral cavity and oropharynx

South

Breems

Richa

et al. 2019

Sci Rep

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Squamous cell carcinomas of the head and neck (HNSCC) arise from mucosal keratinocytes of the upper aero-digestive tract. Despite a common cell of origin and similar driver-gene mutations which divert cell fate from differentiation to proliferation, HNSCC are considered a heterogeneous group of tumors categorized by site of origin within the aero-digestive mucosa, and the presence or absence of HPV infection. Tobacco use is a major driver of carcinogenesis in HNSCC and is a poor prognosticator that has previously been associated with poor immune cell infiltration and higher mutation numbers. Here, we study patterns of mutations in HNSCC that are derived from the specific nucleotide changes and their surrounding nucleotide context (also known as mutation signatures). We identify that mutations linked to DNA adducts associated with tobacco smoke exposure are predominantly found in the larynx. Presence of this class of mutation, termed COSMIC signature 4, is responsible for the increased burden of mutation in this anatomical sub-site. In addition, we show that another mutation pattern, COSMIC signature 5, is positively associated with age in HNSCC from non-smokers and that larynx SCC from non-smokers have a greater number of signature 5 mutations compared with other HNSCC sub-sites. Immunohistochemistry demonstrates a significantly lower Ki-67 proliferation index in size matched larynx SCC compared with oral cavity SCC and oropharynx SCC. Collectively, these observations support a model where larynx SCC are characterized by slower growth and increased susceptibility to mutations from tobacco carcinogen DNA adducts.

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Human papillomavirus-associated squamous cell carcinoma of the larynx or hypopharynx: Clinical outcomes and implications for laryngeal preservation

Cited by 25 publications

References 40 publications

Prevalence of Human Papillomavirus Associated with Head and Neck Squamous Cell Carcinoma in Jordanian Patients

Prevalence of Human Papillomavirus Associated with Head and Neck Squamous Cell Carcinoma in Jordanian Patients

Prognostic role of human papilloma virus status in hypopharyngeal squamous cell carcinoma

Mutation signature analysis identifies increased mutation caused by tobacco smoke associated DNA adducts in larynx squamous cell carcinoma compared with oral cavity and oropharynx

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