Human papillomavirus 16 is an aetiological factor of scrotal cancer

Guimerà, Núria; Alemany, Laia; Halec, Gordana; Pawlita, Michael; Wain, Gerard; Vailén, José Santos Salas; Azike, Jerome Emeka; Jenkins, David; Sanjosé, Sílvia de; Quint, Wim; Bosch, Franz X.

doi:10.1038/bjc.2017.74

Cited by 15 publications

(10 citation statements)

References 30 publications

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“…Conversely, there are limited reports on the detection of HPV mRNA in cutaneous lesions, which we surmised to be most likely due to the inability, or lack of sensitivity of current methods, to detect the presence of the transcripts accurately. Interestingly, HPV16 E6 DNA and E6*I mRNA have been detected in 3/6 cases of primary squamous cell scrotal carcinoma using the QuantiTect Virus Kit and specific primers (Guimera et al, 2017). This might be due to the proximity to the anogenital region where HPV16 is most commonly located.…”

Section: Discussionmentioning

confidence: 99%

Detection of HPV E7 transcription at single-cell resolution in epidermis

Lukowski

Tuong

Noske

et al. 2018

Preprint

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SummaryPersistent human papillomavirus (HPV) infection is responsible for at least 5% of human malignancies. Most HPV-associated cancers are initiated by the HPV16 genotype, as confirmed by detection of integrated HPV DNA in cells of oral and anogenital epithelial cancers. However, single-cell RNA-sequencing (scRNA-seq) may enable prediction of HPV involvement in carcinogenesis at other sites. We conducted scRNA-seq on keratinocytes from a mouse transgenic for the E7 gene of HPV16, and showed sensitive and specific detection of HPV16-E7 mRNA, predominantly in basal keratinocytes. We showed that increased E7 mRNA copy number per cell was associated with increased expression of E7 induced genes. This technique enhances detection of viral transcripts in solid tissue and may clarify possible linkage of HPV infection to development of squamous cell carcinoma.

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Section: Discussionmentioning

confidence: 99%

Detection of HPV E7 transcription at single-cell resolution in epidermis

Lukowski

Tuong

Noske

et al. 2018

Preprint

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“…The analytical sensitivity of the respective assays per reaction is 10,000 copies for HPV70, 1,000 copies for HPV67, and 10–100 copies for the remaining 19 HPV types and for ubC [ 39 ]. The HPV RNA assay has been widely applied and validated as a marker for HPV transformation in carcinoma of the anogenital region, such as the cervix [ 39 , 40 ], vulva [ 41 ], penis [ 42 ], lung [ 43 ], and scrotum [ 44 ], as well as carcinoma of the head and neck [ 30 , 33 ], and specifically oropharynx [ 45 , 46 ], unknown primary of the neck [ 47 , 48 ], larynx [ 27 ], and esophagus [ 49 ].…”

Section: Methodsmentioning

confidence: 99%

Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania

et al. 2018

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BackgroundLimited information is available about the involvement of human papillomavirus (HPV) in head and neck squamous cell carcinomas (HNSCCs) in Romanian patients.ObjectiveTo evaluate the HPV-attributable fraction in HNSCCs collected in Northeastern Romania.Materials and methodsIn total, 189 formalin-fixed paraffin-embedded tissue samples (99 oral cavity tumors, 28 oropharynx, 48 pharynx, and 14 larynx/hypopharynx) were analyzed for HPV DNA and RNA using Luminex-based assays, and for overexpression of p16INK4a (p16) by immunohistochemistry.ResultsOf the 189 cases, 23 (12.2%) were HPV DNA-positive, comprising half of the oropharyngeal cases (14/28, 50.0%) and 9/161 (5.6%) of the non-oropharyngeal cases. HPV16 was the most prevalent HPV type (20/23, 86.9%), followed by HPV18 (5/23, 21.7%) and HPV39 (1/23, 4.3%). Only two (2/189, 1.1%) HNSCC cases were HPV-driven, i.e. positive for both HPV DNA and RNA.ConclusionA very small subset of HNSCC cases within this cohort from Northeastern Romania appeared to be HPV-driven.

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“…E6* expression has also been studied in uncommon HPV-related cancers. In squamous cell scrotal cancer samples, HPV16 E6*I transcripts were found [ 86 ]. Additionally, in breast tumor samples infected with HPV16, E6*I, E6*II, E6^E7 (E6*X), E6^E7*I and E6^E7*II transcripts were detected [ 62 ].…”

Section: E6/e6* Transcription Patternsmentioning

confidence: 99%

The Role of E6 Spliced Isoforms (E6*) in Human Papillomavirus-Induced Carcinogenesis

Olmedo-Nieva

Muñoz-Bello

Contreras‐Paredes

et al. 2018

Viruses

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Persistent infections with High Risk Human Papillomaviruses (HR-HPVs) are the main cause of cervical cancer development. The E6 and E7 oncoproteins of HR-HPVs are derived from a polycistronic pre-mRNA transcribed from an HPV early promoter. Through alternative splicing, this pre-mRNA produces a variety of E6 spliced transcripts termed E6*. In pre-malignant lesions and HPV-related cancers, different E6/E6* transcriptional patterns have been found, although they have not been clearly associated to cancer development. Moreover, there is a controversy about the participation of E6* proteins in cancer progression. This review addresses the regulation of E6 splicing and the different functions that have been found for E6* proteins, as well as their possible role in HPV-induced carcinogenesis.

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Human papillomavirus 16 is an aetiological factor of scrotal cancer

Cited by 15 publications

References 30 publications

Detection of HPV E7 transcription at single-cell resolution in epidermis

Detection of HPV E7 transcription at single-cell resolution in epidermis

Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania

The Role of E6 Spliced Isoforms (E6*) in Human Papillomavirus-Induced Carcinogenesis

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