Human papilloma virus testing in oropharyngeal squamous cell carcinoma: What the clinician should know

Mirghani, Haïtham; Amen, Furrat; Moreau, F.; Guigay, J.; Ferchiou, M.; Melkane, Antoine E.; Hartl, Dana M.; Guily, Jean Lacau St

doi:10.1016/j.oraloncology.2013.10.008

Cited by 84 publications

(94 citation statements)

References 89 publications

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“…Several other methods have been developed, 3 to directly or indirectly identify HPV-related head and neck squamous cell carcinoma, but none of them are ideal ( Table 1). Detection of HPV-DNA can be performed by PCR or in situ hybridization.…”

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confidence: 99%

“…Detection of HPV-DNA can be performed by PCR or in situ hybridization. PCR is highly sensitive but lacks specificity, as it cannot distinguish between an infection truly involved in carcinogenesis and a bystander oral HPV infection, 3,4 which is not uncommon in the general population. 5 On the other hand, detection of HPV-DNA by in situ hybridization has good specificity, as the virus is directly visualized within the tumor cell nuclei, but several authors have noted a lack of sensitivity.…”

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confidence: 99%

“…8,9 Moreover, it is a cost-effective and practical tool that can be used routinely in most histopathological laboratories. 3 Despite these advantages, p16-immunohistochemestry is not reliable enough to be used as a standalone test as its performance may be confounded by a lack of specificity for oncogenic HPV infection. 3 Several authors have reported that ∼ 8-20% of p16-positive oropharyngeal squamous cell carcinomas do not contain HPV-DNA and have a molecular profile that is closer to HPV-negative cancers.…”

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confidence: 99%

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Diagnosis of HPV-driven head and neck cancer with a single test in routine clinical practice

et al. 2015

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Accurate screening of HPV-driven head and neck squamous cell carcinoma is a critical issue. Although there are commercial direct and indirect assays for HPV-related head and neck squamous cell carcinoma, none are ideal. Recently, a novel RNA in situ hybridization test (the RNAscope HPV-test) has been developed for the detection of high-risk HPV E6/E7 mRNA in formalin-fixed paraffin-embedded tissue. However, validation of this assay against the 'gold standard' (identification of high-risk HPV E6/E7 mRNA in fresh-frozen tissue by quantitative real-time (qRT)-PCR) has only been reported by one team. Formalin-fixed paraffin-embedded samples from 50 patients with tonsil or tongue base carcinoma were tested using the RNAscope HPV-test, p16 immunohistochemistry, and chromogenic in situ hybridization for high-risk HPV-DNA. The results were compared with those of qRT-PCR on matched fresh-frozen samples. Compared with the reference test, the sensitivity, specificity, positive, and negative predictive values of the RNAscope HPV-test and of p16 immunohistochemistry were 93%, 94%, 96%, 88% and 96%, 93%, 96%, and 93%, respectively. Five cases were discrepant between the RNAscope HPV-test and p16-immunohistochemisrty. The RNAscope HPV-test demonstrated excellent analytical performance against the 'gold standard' and is easier to interpret than chromogenic in situ hybridization. p16-immunohistochemistry also performed very well, however its main weakness is that it is an indirect marker of the presence of HPV. These data suggest that the RNAscope HPV-test is a promising test that could be developed as a clinical standard for the precise identification of HPV-driven oropharyngeal squamous cell carcinoma.

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Diagnosis of HPV-driven head and neck cancer with a single test in routine clinical practice

et al. 2015

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“…The essay for HPV RNAscope® is designed to detect the mRNA of E6/E7 of high-risk HPV genotypes (e.g. HPV 16,18,31,33,35,52 and 58) using a pool of probes specifi c for these genotypes [32][33][34].…”

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confidence: 99%

“…To date, it is believed that the prevalence of HPV in OPSCC ranges from 50% to 80% at the world level, with sharp variations depending on the study population and the geographical location. However, these estimates could be inaccurate, since the methods of detection are still extremely variable among different laboratories and most of those currently used for screening purposes does not say whether the virus is transcriptionally active in tumors [35].…”

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confidence: 99%

Diagnosing HPV-Related Oropharyngeal Cancers: The Need to Speak a Common Language

Ilardi¹

2017

Glob J Cancer Ther

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RNA Oncoimmune Phenotyping of HPV-Positive p16-Positive Oropharyngeal Squamous Cell Carcinomas by Nodal Status

Stepp

Farquhar

Sheth

et al. 2018

JAMA Otolaryngol Head Neck Surg

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Many HPV-16 and p16-positive tumors are treated as "lower-risk," but they do not have similar genetic compositions at the biological level. The identification of subgroups with unique expression patterns, such as those with nodal metastases, may guide physicians toward alternative or more aggressive therapies. In our study, unguided clustering suggested that that the larger biological characteristics of a tumor could be a better prognostic biomarker.

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Human papilloma virus testing in oropharyngeal squamous cell carcinoma: What the clinician should know

Cited by 84 publications

References 89 publications

Diagnosis of HPV-driven head and neck cancer with a single test in routine clinical practice

Diagnosis of HPV-driven head and neck cancer with a single test in routine clinical practice

Diagnosing HPV-Related Oropharyngeal Cancers: The Need to Speak a Common Language

RNA Oncoimmune Phenotyping of HPV-Positive p16-Positive Oropharyngeal Squamous Cell Carcinomas by Nodal Status

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