Human Papilloma Virus Infection and Overexpression of P53 Protein in Bilharzial Bladder Cancer

Khaled, Hussein M.; Raafat, Amira; Mokhtar, Nadia; Zekri, Abdel Rahman N.; Gaballah, Hussein

doi:10.1177/030089160108700409

Cited by 16 publications

(10 citation statements)

References 18 publications

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“…PBF (buffy coat) from these patients was uniformly negative by both real-time QPCR and MassARRAY (data not shown). Table 5) with older in situ hybridization data (4) and TaqMan data for a standard 40 cycles (left side of Table 5) show that MassARRAY is more sensitive than either in situ hybridization or TaqMan QPCR. The lack of reproducibility of the data on the left side of Table 5 (data not shown) indicates that the TaqMan technique is operating at the limits of its sensitivity and is not accurate.…”

Section: Resultsmentioning

confidence: 92%

“…(i) All 27 schistosomiasis-associated bladder cancers were associated with HPV-16 DNA, whereas a less-sensitive in situ hybridization technique detected only one-half of these associations (4). All samples reacted with the HPV-16 DNA probe and not the HPV-18 DNA probe.…”

Section: Discussionmentioning

confidence: 98%

“…(ii) All 27 bladder cancers due to schistosomiasis were associated with the presence of HPV-16 DNA, which can be detected in tumor and serum but not in PBF. In contrast, no serum HPV- 16 cancer diagnosis ͉ cancer treatment ͉ bilharziasis R ecent studies indicate that the human papillomavirus (HPV) is associated with a significant fraction of cervical (1,2), head͞neck (3), and schistosomiasis-associated bladder cancers (4). Cervical cancers are almost uniformly associated with HPV infection (5).…”

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confidence: 99%

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Sensitive detection of human papillomavirus in cervical, head/neck, and schistosomiasis-associated bladder malignancies

Yang

Khafagi

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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We assayed for the presence of human papilloma virus (HPV) DNA in serum and͞or peripheral blood fraction (PBF) of individuals with cervical, head͞neck, or bladder cancer due to schistosomiasis. Using mass spectroscopy coupled with competitive PCR, HPV DNA was detected at the individual molecule level by using ''Mass-ARRAY'' assays. The resultant sensitivity was superior to real-time fluorescent PCR-based assays, while specificity was maintained. Our principal findings were: (i) Virtually all tested cervical cancers and schistosomiasis-associated bladder cancers, and a plurality of head͞neck cancers, are associated with HPV DNA in the tumor. (ii) All 27 bladder cancers due to schistosomiasis were associated with the presence of HPV-16 DNA, which can be detected in tumor and serum but not in PBF. In contrast, no serum HPV-16 DNA signal was detected in seven individuals with schistosomiasis-associated bladder cancers after surgical removal of the tumor. cancer diagnosis ͉ cancer treatment ͉ bilharziasis R ecent studies indicate that the human papillomavirus (HPV) is associated with a significant fraction of cervical (1, 2), head͞neck (3), and schistosomiasis-associated bladder cancers (4). Cervical cancers are almost uniformly associated with HPV infection (5). In a review of published reports, McKaig et al. (3) found the overall prevalence of HPV DNA in head and neck tumors to be 35%. More recently, Gillison et al. (6) used quantitative PCR (QPCR) to confirm these findings in a large study of 253 tumor samples. They detected HPV DNA in 25% of specimens. Khaled et al. (4) found that nearly 50% of schistosomiasis-caused bladder cancers had HPV DNA by in situ hybridization. This body of work argues that HPV could be a useful tag for tracking a considerable fraction of cervical, head͞neck, and schistosomiasis-associated bladder cancers.HPV types 16 and 18 are among the ''high-risk'' viral types, because their presence is associated with preneoplastic lesions and carcinomas. In contrast, the ''low-risk'' types, most commonly types 6 and 11, are typically associated with benign lesions. The oncogenic potential of HPV is principally due to two viral oncoproteins, E6 and E7. Differences in oncogenic potential among HPV types have been attributed to type-specific differences in the E6 and E7 proteins (7). The E6 protein of oncogenic HPV strains has been shown to interact with the p53 protein and promote its degradation via a ubiquitin-dependent pathway (7). The E7 oncoprotein, similarly, can complex with the retinoblastoma (Rb) protein and inactivate it (8). Both p53 and Rb are important tumor suppressor genes whose products regulate the cell cycle, orchestrate DNA repair processes, and are involved with programmed cell death or apoptosis. Disruption of these tumor suppressor proteins by HPV leads to propagation of mutational changes and cell immortalization.Since the work of Anker, Sidransky, and coworkers (9-11) established that abnormal genomic DNA can be detected in serum of cancer patients, the technique of examin...

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Section: Resultsmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

See 1 more Smart Citation

Sensitive detection of human papillomavirus in cervical, head/neck, and schistosomiasis-associated bladder malignancies

Yang

Khafagi

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…These results suggest that 'high-risk' HPV 16 and 18 carries a risk for the development of malignancy in the urinary tract. HPV positivity has been found more frequently in squamous than in transitional cell carcinoma [155]. Among patients with transitional cell carcinoma, HPV 16 [147] and 18 [150,162] have been the most frequently detected HPV types, indicating that 'high-risk' HPVs play a causative role in transitional cell carcinoma of bladder.…”

Section: Hpv and Urothelial Cancermentioning

confidence: 99%

“…The overall and 'high-risk' HPV infections in neoplastic specimens were distributed almost equally in male and female patients [180]. Several studies have shown geographical differences [155]. Koilocytosis has been shown to be a good morphological marker for HPV DNA in the urothelium [166], with a positive predictive value of 84.6% [146].…”

Section: Hpv and Urothelial Cancermentioning

confidence: 99%

Vaccination against Human Papilloma Virus (HPV): Epidemiological Evidence of HPV in Non-genital Cancers

Mammas

Sourvinos

Zaravinos

et al. 2010

Pathol. Oncol. Res.

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Recently, the vaccine against human papillomavirus (HPV) was introduced in the national vaccination programmes of several countries worldwide. The established association between HPV and the progression of cervical neoplasia provides evidence of the expected protection of the vaccine against cervical cancer. During the last two decades several studies have also examined the possible involvement of HPV in non-genital cancers and have proposed the presence of HPV in oesophageal, laryngeal, oropharyngeal, lung, urothelial, breast and colon cancers. The possible involvement of HPV in these types of cancer would necessitate the introduction of the vaccine in both boys and girls. However, the role of HPV in the pathogenesis of these types of cancer has yet to be proven. Moreover, the controversial evidence of the possible impact of the vaccination against HPV in the prevention of non-genital cancers needs to be further evaluated. In this review, we present an overview of the existing epidemiological evidence regarding the detection of HPV in non-genital cancers.

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Parasites

Azzouni

Diab

2017

Holland‐Frei Cancer Medicine

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Overview Many parasites were studied for a possible role in oncogenesis. Schistosoma haematobium was proved to play an important role in developing urinary bladder cancer. Other Schistosoma species, Schistosoma japonicum is classified as a colorectal carcinogen especially in the Far East. Other helminths Clonorchis and Opisthorchis are proved to induce hepatobiliary cancer. In Africa, a strong correlation between Ebstein–Barr virus infection and Burkitt lymphoma is present, with an evident enhancing role for Plasmodium falciparum . Chronic inflammation was incriminated to be the most accepted mechanism for parasite‐induced cancer; however, the roles of certain carcinogens, oncogenes, DNA mutations, and others were all approved as mechanisms enhancing carcinogenesis in parasitic infections. Strikingly, despite the above‐mentioned data, it seemed that certain parasites can modulate the host immune response in a manner that could lead to cancer regression or prevention. This is in the prospect of revaluation of the clinical importance of infectious agents; an issue that requires future concern.

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Human Papilloma Virus Infection and Overexpression of P53 Protein in Bilharzial Bladder Cancer

Cited by 16 publications

References 18 publications

Sensitive detection of human papillomavirus in cervical, head/neck, and schistosomiasis-associated bladder malignancies

Sensitive detection of human papillomavirus in cervical, head/neck, and schistosomiasis-associated bladder malignancies

Vaccination against Human Papilloma Virus (HPV): Epidemiological Evidence of HPV in Non-genital Cancers

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