Human oviductal epithelial cells express Toll-like receptor 3 and respond to double-stranded RNA: Fallopian tube-specific mucosal immunity against viral infection

Nasu, Kaei; Itoh, Hiroko; Yuge, Akitoshi; Nishida, Masakazu; Narahara, Hisashi

doi:10.1093/humrep/del385

Cited by 19 publications

(22 citation statements)

References 32 publications

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“…Another recent study demonstrated proinflammatory responses by human oviductal epithelial cells upon exposure to poly(I:C) (85). Similar to the Itoh et al study described above, the authors in this paper cultured the oviductal epithelial cells to a confluent monolayer on plastic.…”

Section: Discussionsupporting

confidence: 70%

Antiviral responses of human Fallopian tube epithelial cells to toll-like receptor 3 agonist poly(I:C)

Ghosh¹,

Schaefer²,

Fahey³

et al. 2008

Fertility and Sterility

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Objective-To examine the expression of toll-like receptors (TLR) by primary human Fallopian tube epithelial cells (FTEC) and to determine whether exposure to the TLR3 agonist poly(I:C) would induce an antiviral response.Design-Tissue culture study. Setting-University Medical Center. Patient(s)-Pre-menopausal women undergoing hysterectomy.Intervention(s)-Primary human FTEC were grown to confluence and high transepithelial resistance and treated with TLR agonists. Conditioned media was collected and RNA was extracted and analyzed for the expression of cytokines, chemokines and antimicrobial genes.Main Outcome Measure(s)-RNA was analyzed by real-time RT-PCR and protein levels were assessed by ELISA. Result(s)-The FTEC were demonstrated to express TLR1-9 but not 10. Treatment of FTEC with TLR3 agonist poly(I:C) resulted in increased expression of IL-8, TNF-α, human β-defensin 2, interferon beta, and interferon stimulated genes myxovirus resistance gene 1, 2′,5′-oligoadenylate synthetase, and protein kinase R. Additionally, FTEC exposed to poly(I:C) also resulted in the induction of TLR 2, 3, and 7.Conclusion(s)-Our results suggest that FTEC are sensitive to viral infection and/or exposure to viral dsRNA and can respond by secreting proinflammatory cytokines that mediate the initiation of an inflammatory response as well as expressing genes that can directly inhibit viral replication.

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Section: Discussionsupporting

confidence: 70%

Antiviral responses of human Fallopian tube epithelial cells to toll-like receptor 3 agonist poly(I:C)

Ghosh¹,

Schaefer²,

Fahey³

et al. 2008

Fertility and Sterility

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“…TLRs have been shown by our group and others to be expressed in the human FRT [180][181][182][183]. These studies demonstrated a site-specific pattern of expression of TLRs in the FRT.…”

Section: Expression Of Tlr In the Frtsupporting

confidence: 54%

Innate Immunity in the Female Reproductive Tract: Role of Sex Hormones in Regulating Uterine Epithelial Cell Protection Against Pathogens

Ochiel

Fahey²,

Ghosh³

et al. 2008

CWHR

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The mucosal immune system in the upper female reproductive tract is uniquely prepared to maintain a balance between the presence of commensal bacteria, sexually transmitted bacterial and viral pathogens, allogeneic spermatozoa, and an immunologically distinct fetus. At the center of this dynamic system are the epithelial cells that line the Fallopian tubes, uterus, cervix and vagina. Epithelial cells provide a first line of defense that confers continuous protection, by providing a physical barrier as well as secretions containing bactericidal and virucidal agents. In addition to maintaining a state of ongoing protection, these cells have evolved to respond to pathogens, in part through Toll-like receptors (TLRs), to enhance innate immune protection and, when necessary, to contribute to the initiation of an adaptive immune response. Against this backdrop, epithelial cell innate and adaptive immune function is modulated to meet the constraints of procreation. The overall goal of this review is to focus on the dynamic role of epithelial cells in the upper reproductive tract, with special emphasis on the uterus, to define the unique properties of these cells as they maintain homeostasis in preparation for successful fertilization and pregnancy while at the same time confer protection against sexually transmitted infections, which threaten to compromise women's reproductive health and survival. By understanding the nature of this protection and the ways in which innate and adaptive immunity are regulated by sex hormones, these studies provide the opportunity to contribute to the foundation of information essential for ensuring reproductive health.

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“…Endometrial, cervical and Fallopian tube epithelial cells have both been shown to respond to the TLR3 ligand, poly I:C, with increased cytokine output (Lesmeister et al 2005, Andersen et al 2006, Ghosh et al 2008, Nasu et al 2007. Fallopian tube fibroblasts and endometrial epithelial and stromal cells have been shown to increase inflammatory mediator output in response to LPS (lipopolysaccharide), a TLR4 ligand (Hirata et al 2005, Itoh et al 2006.…”

Section: Toll-like Receptors (Tlrs)mentioning

confidence: 99%

Innate immunity and disorders of the female reproductive tract

Horne¹,

Stock²,

King³

2008

Reproduction

136

129

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Sexually transmitted infections, and their associated sequelae, such as tubal infertility, ectopic pregnancy and preterm labour, are a major worldwide health problem. Chlamydia trachomatis infection is thought to be the leading global cause of tubal infertility and tubal ectopic pregnancy. Preterm birth occurs in around 10% of all deliveries, and nearly 30% of preterm deliveries are associated with intrauterine infection. The mucosal innate immune system of the female reproductive tract has evolved to eliminate such sexually transmitted pathogens whilst maintaining its ability to accommodate specialized physiological functions that include menstruation, fertilization, implantation, pregnancy and parturition. The aim of this review was to describe the role and distribution of key mediators of the innate immune system, the natural antimicrobial peptides (secretory leukocyte protease inhibitor, elafin and the defensins) and the pattern recognition toll-like receptors in the normal female reproductive tract and in the context of these pathological processes.

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Human oviductal epithelial cells express Toll-like receptor 3 and respond to double-stranded RNA: Fallopian tube-specific mucosal immunity against viral infection

Cited by 19 publications

References 32 publications

Antiviral responses of human Fallopian tube epithelial cells to toll-like receptor 3 agonist poly(I:C)

Antiviral responses of human Fallopian tube epithelial cells to toll-like receptor 3 agonist poly(I:C)

Innate Immunity in the Female Reproductive Tract: Role of Sex Hormones in Regulating Uterine Epithelial Cell Protection Against Pathogens

Innate immunity and disorders of the female reproductive tract

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