Human oviductal cells produce a factor(s) that maintains the motility of human spermatozoa in vitro

Yao, Yuanqing; Ho, Pak‐Chung; Yeung, William S.B.

doi:10.1016/s0015-0282(99)00563-4

Cited by 27 publications

(11 citation statements)

References 25 publications

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“…In addition, the conditioned media from cultures of oviductal cells or tissue explants from different species were used to study the effects of the oviductal secretion on gamete function (Verhage et al 1988, Yeung et al 1994, Yao et al 2000, Quintero et al 2005, Munuce et al 2009, Zumoffen et al 2010.…”

Section: Oviductal Secretion and Sperm Functionmentioning

confidence: 99%

Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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Experimental evidence from the last 30 years supports the fact that the oviduct is involved in the modulation of the reproductive process in eutherian mammals. Oviductal secretion contains molecules that contribute to regulation of gamete function, gamete interaction, and the early stages of embryo development. The oviductal environment would act as a sperm reservoir, maintaining sperm viability, and modulating the subpopulation of spermatozoa that initiates the capacitation process. It could also contribute to prevent the premature acrosome reaction and to reduce polyspermy. Many studies have reported the beneficial effects of the oviductal environment on fertilization and on the first stages of embryo development. Some oviductal factors have been identified in different mammalian species. The effects of oviductal secretion on the reproductive process could be thought to result from the dynamic combined action (inhibitory or stimulatory) of multiple factors present in the oviductal lumen at different stages of the ovulatory cycle and in the presence of gametes or embryos. It could be hypothesized that the absence of a given molecule would not affect fertility as its action could be compensated by another factor with similar functions. However, any alteration in this balance could affect certain events of the reproductive process and could perhaps impair fertility. Thus, the complexity of the reproductive process warrants a continuous research effort to unveil the mechanisms and factors behind its regulation in the oviductal microenvironment.

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Section: Oviductal Secretion and Sperm Functionmentioning

confidence: 99%

Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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“…Indeed, an increase of isthmus contraction frequency after 2 h of mating in rabbits has been reported (Bourdage and Halbert, 1980). The mere presence of spermatozoa is enough to stimulate a number of responses in oviductal epithelial cells (Murray and Smith, 1997;Yao et al, 2000). One contradictory observation from this work was that the force of contraction increased in the uterine horns following addition of seminal plasma, with no changes in the electrical activity.…”

Section: Discussionmentioning

confidence: 72%

Characterization of electrical and mechanical activities of rabbit uterus associated with the presence of capacitated and non-capacitated spermatozoa

2013

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To investigate the effects capacitated spermatozoa may exert upon motility of the rabbit uterus, both contractility and electrical activity (frequency and intensity) were measured in 3 distinctive uterine segments of anaesthetized does: horn (UH), uterotubal junction (UTJ) and tube (UT) after 1) natural mating, 2) infusion of either seminal plasma or PBS, 3) infusion of either capacitated or non-capacitated spermatozoa. Basal values were: 17.1, 15.7, 16.4 g (contractility, P>0.05); 3.5, 3.5, 3.4 Hz (frequency, P>0.05); 0.49, 0.50, 0.57 μV (intensity, P>0.05) for UH, UTJ, UT, respectively. Seminal plasma caused an increase (P<0.05) in the UH contractility: 26.3 vs. 11.7 (natural mating) and 17.0 g (PBS); it also caused a decrease (P<0.05) in electrical intensity at the UTJ: 0.24 vs. 0.67 (natural mating) and 0.58 µV (PBS). The presence of either capacitated or non-capacitated spermatozoa caused no changes in contractility and electrical frequency in any of the uterine segments. However, there was a change in electrical intensity at UTJ (0.37 vs. 0.57 μV for non-capacitated and capacitated spermatozoa, respectively; P<0.05). There were also differences between segments by treatment: UTJ (0.37) vs. UT (0.59 µV) for non-capacitated; UH (0.46) vs. UT (0.71 µV) for capacitated spermatozoa (P<0.05). In conclusion, use of this experimental model showed that uterine electrical activity was slightly modified by the presence of capacitated spermatozoa.

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“…Resultados semelhantes foram descritos por Kumaresan et al [34] que observaram um aumento da viabilidade de espermatozoides de búfalos incubados com proteínas de oviduto. Yao et al [35] também descreveram um aumento da motilidade de espermatozoides humanos cocultivados com células epiteliais de oviduto ou incubados com seu meio condicionado.…”

Section: Resultsunclassified

Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados

et al. 2015

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Cómo citar este artículo: Valleriote P, Paes de Carvalho C, Dubeibe DF, Mogollón-Waltero ÉM, Burla-Dias, ÂJ. Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados. Spei Domus. 2015;11(22) Resumo. Introdução: nos bovinos, o oviduto é fundamental nos processos de maturação, fertilização e aquisição de competências para o adequado desenvolvimento do embrião até blastocisto. Visto que as células do revestimento interno do oviduto proporcionam substâncias que afetam positivamente os espermatozoides, à medida que atravessam o trato genital da fêmea, os componentes da superfície são modificados ou removidos pelas secreções ali presentes; ditas modificações podem incluir depleção do colesterol na superfície espermática, alteração nos glicosaminoglicanos e mudanças nos íons. Metodologia: Objetivou-se avaliar a adição de meio de cultura de células de oviduto bovino sobre parâmetros espermáticos como motilidade, vigor e integridade de membrana citoplasmática. Os espermatozoides foram incubados com 164 μl de Talp-sp-Uso no grupo controle (T Ct ); 161,5 μl de Talp-sp-Uso, acrescido de 2,5 μl de heparina (20µg/ml) no tratamento 1 (T Hep ) e 128 μl de Talp-sp uso, acrescido de 36 μl de meio de cultivo com secreções de células do oviduto bovino no Tratamento 2 (T Ov ). Foram avaliados no tempo zero (para mostrar que os meios dos tratamentos não prejudicaram os espermatozoides) e após três horas de incubação. Resultados: não foram encontradas diferenças significativas na motilidade progressiva, vigor e integridade de membrana celular entre tratamentos. Entretanto, espermatozoides cultivados com secreções de células do oviduto apresentaram maior percentagem de motilidade total às três horas de cultivo, em comparação a espermatozoides cultivados na presença de heparina. Conclusão: as secreções de células do oviduto não afetam a motilidade progressiva nem o vigor ou a integridade da membrana citoplasmática dos espermatozoides, porém prolongam a motilidade dessas células.Palavras-chave: capacitação espermática, células do oviduto, meio condicionado.

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Human oviductal cells produce a factor(s) that maintains the motility of human spermatozoa in vitro

Cited by 27 publications

References 25 publications

Oviductal secretion and gamete interaction

Oviductal secretion and gamete interaction

Characterization of electrical and mechanical activities of rabbit uterus associated with the presence of capacitated and non-capacitated spermatozoa

Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados

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