Human originated bacteria, Lactobacillus rhamnosus PL60, produce conjugated linoleic acid and show anti-obesity effects in diet-induced obese mice

Lee, Hui Young; Park, Jong‐Hwan; Seok, Seung Hyeok; Baek, Min Won; Kim, Dong-Jae; Lee, Ki-Eun; Paek, Kyungsoo; Lee, Yeonhee; Park, Jae Hak

doi:10.1016/j.bbalip.2006.05.007

Cited by 307 publications

(232 citation statements)

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“…The relatively low amount of t10, c12 CLA produced by this strain (1.9 mg ml 21 was detected in the sera) was enough to produce an anti-obesity effect. Moreover, liver steatosis, a well-known side effect of t10, c12 CLA, was not observed following L. rhamnosus PL60 treatment in this study (Lee et al, 2006a). Thus, the lower dose of CLA produced by a probiotic strain may be a reliable solution to control the adverse effects caused by dietary t10, c12 CLA, i.e.…”

Section: Discussionmentioning

confidence: 52%

“…The majority of these studies have demonstrated production of c9, t11 CLA from linoleic acid, while only a few bacterial species have been reported to produce t10, c12 CLA, i.e. Propionibacterium acnes isolated from mouse caecum (Verhulst et al, 1987), the rumen bacterium Megasphaera elsdenii (Kim et al, 2002b) and the human-derived Lactobacillus rhamnosus PL60 and Lactobacillus plantarum PL62 (Lee et al, 2006a;Lee et al, 2007). However, only a few studies have established CLA production in vivo following ingestion of a CLA-producing bacterium.…”

Section: Introductionmentioning

confidence: 99%

“…However, only a few studies have established CLA production in vivo following ingestion of a CLA-producing bacterium. For example, the in vivo production of t10, c12 CLA by Lactobacillus rhamnosus PL60 and Lactobacillus plantarum PL62 was reported (Lee et al, 2006a(Lee et al, , 2007, and we recently demonstrated that feeding different animal species a c9, t11 CLA-producing Bifidobacterium strain of human origin (Bifidobacterium breve NCIMB 702258), in combination with dietary linoleic acid, resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 CLA in the liver (Wall et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

“…2003) and has been demonstrated to alter body composition by reducing the fat content and increasing the lean body mass in animal studies, and also in some human studies (Blankson et al, 2000;Smedman & Vessby, 2001;Thom et al, 2001). It was reported by Lee et al (2006a) that t10, c12 CLA was produced in vivo when the humanoriginated bacterium L. rhamnosus PL60 was fed to dietinduced obese mice. The relatively low amount of t10, c12 CLA produced by this strain (1.9 mg ml 21 was detected in the sera) was enough to produce an anti-obesity effect.…”

mentioning

confidence: 99%

“…It was reported by Lee et al (2006a) that t10, c12 CLA was produced in vivo when the humanoriginated bacterium L. rhamnosus PL60 was fed to dietinduced obese mice. The relatively low amount of t10, c12 CLA produced by this strain (1.9 mg ml 21 was detected in the sera) was enough to produce an anti-obesity effect.…”

mentioning

confidence: 99%

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Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice

Rosberg-Cody

Stanton

O’Mahony³

et al. 2011

Microbiology

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We have previously demonstrated that oral administration of a metabolically active Bifidobacterium breve strain, with ability to form cis-9, trans-11 conjugated linoleic acid (CLA), resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 CLA and omega-3 (n-3) fatty acids in liver and adipose tissue. In this study, we investigated whether a recombinant lactobacillus expressing linoleic acid isomerase (responsible for production of t10, c12 CLA) from Propionibacterium acnes (PAI) could influence the fatty acid composition of different tissues in a mouse model. Linoleic-acid-supplemented diets (2 %, w/w) were fed in combination with either a recombinant t10, c12 CLA-producing Lactobacillus paracasei NFBC 338 (Lb338), or an isogenic (vector-containing) control strain, to BALB/c mice for 8 weeks. A third group of mice received linoleic acid alone (2 %, w/w). Tissue fatty acid composition was assessed by GLC at the end of the trial. Ingestion of the strain expressing linoleic acid isomerase was associated with a 4-fold increase (P,0.001) in t10, c12 CLA in adipose tissues of the mice when compared with mice that received the isogenic non-CLA-producing strain. The livers of the mice that received the recombinant CLA-producing Lb338 also contained a 2.5-fold (albeit not significantly) higher concentration of t10, c12 CLA, compared to the control group. These data demonstrate that a single gene (encoding linoleic acid isomerase) expressed in an intestinal microbe can influence the fatty acid composition of host fat. INTRODUCTIONEvidence is emerging to support the concept that the enteric microbiota can exert profound effects on human health and disease, involving complex host-bacteria interactions that are as yet poorly understood. The gut microbiota is important to the host with regard to metabolic functions, providing nutrients and conferring an ability to resist bacterial infections (Marchesi & Shanahan, 2007;Wilks, 2007), as well as playing a dominant role in the education of the intestinal mucosal immune responses. Furthermore, the enteric microbiota has been shown to exert effects on disease processes outside the gut, including an impact on obesity and non-alcoholic fatty liver disease (Dumas et al., 2006;Marchesi & Shanahan, 2007).Conjugated linoleic acid (CLA) is a beneficial bacterial metabolite formed via microbial isomerization of linoleic acid. CLA is a collective term describing different isomers of linoleic acid with conjugated double bonds, the cis-9, trans-11 (c9, t11) CLA isomer being the most abundant form and the t10, c12 CLA isomer accounting for~1 % of total milk fat CLA (Jensen, 2002). It has been reported that t10, c12 CLA is the most potent isomer in terms of potential to prevent cell proliferation and induce apoptosis in cancer cells (Cho et al., 2005(Cho et al., , 2006Kim et al., 2002a; Lee et al., 2006b; Ochoa et al., 2004). t10, c12 CLA is also associated with decreased body fat and increased lean body mass in various animal...

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Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice

Rosberg-Cody

Stanton

O’Mahony³

et al. 2011

Microbiology

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Acid lactic lactobacilli as a biotechnological toll to improve food quality and human health

Sousa

Rama

Souza

et al. 2019

Biotechnology Progress

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The genus Lactobacillus has been widely used in food industry as starter or adjunct culture due to its probiotic features. Its biotechnological features improve the spectrum of uses of lactobacilli, which can affect its applicability directly. In this sense, this literature review gathers information and discusses the biotechnological potential of technological/probiotic lactobacilli aiming to improve food quality and human health. The primary and secondary metabolism generates specific substances, such as organic acids, carbon dioxide, hydrogen peroxide, diacetyl, fatty acids, and bacteriocins, which are determinant due to their probiotic potential, antimicrobial activity, and the development of new food flavors. In order to become industrially and commercially attractive, it is necessary develop a large‐scale process with lower production costs.

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Omics for Understanding the Gut‐Liver‐Microbiome Axis and Precision Medicine

Khalsa

Duffy

Riscuta

et al. 2017

Clinical Pharm in Drug Dev

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Human metabolic disease opens a new view to understanding the contribution of the intestinal microbiome to drug metabolism and drug-induced toxicity in gut-liver function. The gut microbiome, a key determinant of intestinal inflammation, also plays a direct role in chronic inflammation and liver disease. Gut bacterial communities directly metabolize certain drugs, reducing their bioavailability and influencing individual variation in drug response. In addition, some microbiome-produced compounds may affect drug pharmacokinetics and pharmacodynamics via altered expression of metabolizing enzymes and drug transporters or genes coding for drug target proteins, drug response phenotypes, and disease states. Molecular-based high-throughput technologies are providing novel insight about host-gut microbiome interactions, homeostasis, and xenobiotic effects associated with wide variation in efficacy or toxicity in humans. It is envisioned that future approaches to treating and preventing liver disease will benefit from in-depth studies of the liver-microbiome axis. Thus, the microbiome shares a fundamental role in human physiology with various organ systems, and its importance must be considered in the rapid evolution of precision medicine. A new emerging perspective of understanding the effect of the gut microbiome on human response to drugs would be indispensable for developing efficacious, safe, and cost-effective precision therapies.

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Human originated bacteria, Lactobacillus rhamnosus PL60, produce conjugated linoleic acid and show anti-obesity effects in diet-induced obese mice

Cited by 307 publications

References 40 publications

Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice

Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice

Acid lactic lactobacilli as a biotechnological toll to improve food quality and human health

Omics for Understanding the Gut‐Liver‐Microbiome Axis and Precision Medicine

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