Human oestrogen receptors: differential expression of ERalpha and beta and the identification of ERbeta variants

Scobie, Graeme; MacPherson, Sarah E.; Millar, Michael; Groome, Nigel P.; Romana, Panagiota G; Saunders, Philippa T. K.

doi:10.1016/s0039-128x(02)00047-8

Cited by 81 publications

(64 citation statements)

References 63 publications

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“…In addition, the present study indicated that high cERβ5 expression in thymic tumors was correlated with longer OS and PFS, which was in accordance with previous results (18,20,21,23,28,29). However, the fact that cERβ5 was overexpressed in thymic tumors, while having an inhibiting biological effect, suggested that cERβ5 may be involved in other functions of the thymic tumor development and progression.…”

Section: Discussionsupporting

confidence: 92%

Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression

Wang

et al. 2015

Oncology Letters

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Abstract. A number of previous studies have reported that sex steroid hormones, including estrogens, are involved in the regulation of the thymic function. The aim of the present study was to investigate the expression of estrogen receptor β5 (ERβ5) in thymic tumors and the correlation between ERβ5 expression and thymoma biological characteristics. The expression levels of ERβ5 in thymic epithelial tumors was evaluated in 103 patents using immunohistochemical staining and reverse transcription-quantitative polymerase chain reaction. In addition, an indirect immunofluorescence assay was performed to evaluate the ERβ5 expression levels in the TC1889 and T1682 cell lines. The survival outcome was estimated using Kaplan-Meier plots. The results indicated that ERβ5 expression was mainly located in the thymic tumor cell cytoplasm (87.37%; 90/103 cases) and overexpression was observed in thymic tumors compared with normal thymic tissues (P=0.001). Using the Kruskal-Wallis test, a statistically significant association was observed between cytoplasmic ERβ5 (cERβ5) expression and thymic tumor subtypes (P=0.024) and stages (P=0.003 and R=-0.376). The Kaplan-Meier plots revealed that cERβ5 expression was significantly associated with improved overall and progression-free survival (P=0.008 and P=0.004, respectively). The present study suggested that overexpression of cERβ5 may indicate an improved prognosis and may be involved in the underlying mechanism through which estrogen inhibits thymoma and thymic carcinoma development.

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Section: Discussionsupporting

confidence: 92%

Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression

Wang

et al. 2015

Oncology Letters

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“…We have used our ERb1 specific monoclonal antibody to immunolocalize ERb1 in a series of breast cancers as well as in other human and primate tissues (Saunders et al, 2000;Scobie et al, 2001). The present study has demonstrated the presence of ERb1 in cell nuclei not only the malignant epithelium but also non-malignant elements of most breast cancers.…”

Section: Molecular and Cellular Pathologymentioning

confidence: 58%

“…The need to use frozen sections clearly limits the utility of these antibodies and highlights an important difference with the reagent used in the present study which appears capable of identifying ERb1 in material fixed by formalin, methacarn (unpublished observations) or Bouins (Saunders et al, 2000). In studies using fixed samples from human tissues including ovary, placenta, vas deferens, testis and endometrium we have used monoclonal and polyclonal antibodies to localize ERb proteins (Saunders et al, 2000;Critchley et al, 2001;Scobie et al, 2001). In all cases we find the protein to be nuclear in location in agreement with the findings using fixed tissues of human breast (present study) the only exceptions being dividing cells, and some myoid cell types where background staining of the cytoplasm associated with the secondary antibodies was a problem.…”

Section: Molecular and Cellular Pathologymentioning

confidence: 98%

“…The most prominent proteins other than full length ERb1 migrated between 30 and 36 Kd these could represent use of alternative start sites, translation from an exon 2 deleted mRNA (*35 Kd) or translation of protein from mRNA deleted for both exons 5 and 6 (AF074599) which is predicted to be *43 Kd (short) or *49 Kd (long) from the mRNA sequence. It is notable that mRNAs corresponding to alternatively spliced forms of ERb have been detected in breast cancer tissues and cell lines (Lu et al, 1998;Moore et al, 1998;Vladusic et al, 1998;Iwao et al, 2000) as well as in normal human tissues (Ogawa et al, 1998b;Scobie et al, 2001). Furthermore, monoclonal antibodies directed against the N terminus of ERb have detected expression of proteins other than full length ERb in breast cancer cell lines (Fuqua et al, 1999) which might have been formed by translation of alternatively spliced mRNAs.…”

Section: Molecular and Cellular Pathologymentioning

confidence: 99%

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Expression of oestrogen receptor beta (ERβ1) protein in human breast cancer biopsies

et al. 2002

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Oestrogen action is mediated via specific receptors that act as ligand-activated transcription factors. A monoclonal antibody specific to the C-terminus of human oestrogen receptor beta has been characterized and the prevalence of expression of oestrogen receptor beta protein investigated in a well defined set of breast cancers. Reverse transcription-polymerase chain reaction analysis of RNA from tissue biopsies detected oestrogen receptor beta in all samples examined. The anti-oestrogen receptor beta antibody cross reacted specifically with both long (*59 Kd) and short (*53 Kd) forms of recombinant oestrogen receptor beta. Western blot analysis of breast tumours contained both forms of oestrogen receptor beta protein although in some samples lower molecular weight species (32 -45 Kd) were identified. Fifty-one breast cancer biopsies were examined using immunohistochemistry; 41 (80%) were immunopositive for oestrogen receptor alpha, 48 (94%) were immunopositive for oestrogen receptor beta and 38 (74.5%) co-expressed both receptors. Expression of oestrogen receptor beta was exclusively nuclear and occurred in multiple cell types. There was no quantitative relationship between staining for the two ERs although in tumours in which both receptors were present immunoexpression of oestrogen receptor alpha was invariably more intense. The significance of oestrogen receptor beta protein expression in breast cancers to therapy remains to be determined but the availability of a well characterized antibody capable of detecting oestrogen receptor beta in archive material will facilitate the process.

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“…Therefore, ER␤ could be the receptor mediating the effect of estrogens in human testes. The recent discovery of different human ER␤ isoforms with distinct cell expression patterns (Gaskell et al, 2002;Saunders et al, 2002;Scobie et al, 2002) makes the interpretation of estrogen receptor expression in male gonads more difficult.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of estrogen receptors (ERα/ERβ) in testis of mature and immature pigs

et al. 2004

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High affinity estrogen receptors (ERs) mediate estrogen action in male reproductive tissues. The objective of the present study was the immunolocalization of estrogen receptor ␣ and estrogen receptor ␤ in immature and mature testes of pig, a species in which the role of estrogens on gonadal function is scarcely known. Testes from 3 and 18 month-old pigs were investigated. Immunohistochemistry was performed on paraffin embeddedtissues using both mouse anti-human monoclonal IgG ER␣ and IgG ER␤ 1 isoform. Western blot analysis demonstrated antibody specificity. ER␣ staining was not observed in immature testes, but it was detected in spermatogonia, spermatocytes and in the most Leydig cells of mature testes. ER␤ immunoreactivity was observed in spermatogonia and Leydig cells of immature gonads, while it was clearly detected in spermatogonia and in spermatocytes of adult pig testes. The differential ER␣/ER␤ expression in germ and somatic cells of the gonads suggest a role of estrogens in function and in development of pig testis.

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Human oestrogen receptors: differential expression of ERalpha and beta and the identification of ERbeta variants

Cited by 81 publications

References 63 publications

Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression

Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression

Expression of oestrogen receptor beta (ERβ1) protein in human breast cancer biopsies

Differential expression of estrogen receptors (ERα/ERβ) in testis of mature and immature pigs

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