Human Oct-1L isoform has tissue-specific expression pattern similar to Oct-2

Luchina, N N; Krivega, Ivan; Pankratova, E. V.

doi:10.1016/s0165-2478(02)00179-7

Cited by 26 publications

(18 citation statements)

References 25 publications

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“…The obtained data, presented in Figure 1B and C, show that the abundantly expressed Oct-1A is the main Oct-1 transcript, since in most cells its level roughly equals to that of the total Oct-1 mRNA. A high expression level of Oct-1L was found in brain and leukocytes, similar to that shown previously (31), and in the Burkitt's lymphoma cell lines Namalwa and Raji (Figure 1C), where it exceeds the level of Oct-1A. Oct-1X transcript was detected at a very low level (about 5%) relative to Oct-1A.…”

Section: Resultssupporting

confidence: 88%

“…The Oct-1A transcript (NM_002697) contains an open reading frame for Oct-1A protein (766 aa, UniProt P14859-6) which represents the longest form of Oct-1 comprising all the internal exons (30). The Oct-1L (AY113189) (755 aa, UniProt P14859-2) transcript is tissue-specific (31) while Oct-1X mRNA, which we described earlier (KT438684.1) is expressed at a low level in most tissues (29). Still, the presence of specific Oct-1 protein isoforms in mammalian tissues was not investigated and their functions are unknown.…”

Section: Introductionmentioning

confidence: 79%

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Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes

et al. 2016

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Oct-1 transcription factor has various functions in gene regulation. Its expression level is increased in several types of cancer and is associated with poor survival prognosis. Here we identified distinct Oct-1 protein isoforms in human cells and compared gene expression patterns and functions for Oct-1A, Oct-1L, and Oct-1X isoforms that differ by their N-terminal sequences. The longest isoform, Oct-1A, is abundantly expressed and is the main Oct-1 isoform in most of human tissues. The Oct-1L and the weakly expressed Oct-1X regulate the majority of Oct-1A targets as well as additional sets of genes. Oct-1X controls genes involved in DNA replication, DNA repair, RNA processing, and cellular response to stress. The high level of Oct-1 isoforms upregulates genes related to cell cycle progression and activates proliferation both in Namalwa Burkitt's lymphoma cells and primary human fibroblasts. It downregulates expression of genes related to antigen processing and presentation, cytokine-cytokine receptor interaction, oxidative metabolism, and cell adhesion, thus facilitating pro-oncogenic processes.

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 79%

Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes

et al. 2016

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“…Both Oct-1 and Oct-2 are characterized as octamer binding proteins. Oct-1 is ubiquitously expressed, but with some isoforms expressing in a tissue-specific manner (23,42). Although Oct-2 is expressed predominantly in B cells (30,31), it is also found in neuronal cells (13), T cells (18), and macrophages (21,22,28).…”

Section: Discussionmentioning

confidence: 99%

The essential role of Oct-2 in LPS-induced expression of iNOS in RAW 264.7 macrophages and its regulation by trichostatin A

Lin

et al. 2009

American Journal of Physiology-Cell Physiology

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Lu S, Wu H, Lin Y, Chang S. The essential role of Oct-2 in LPSinduced expression of iNOS in Raw 264.7 macrophages and its regulation by trichostatin A. Am J Physiol Cell Physiol 296: C1133-C1139, 2009. First published March 11, 2009 doi:10.1152/ajpcell.00031.2009.-This article reports on a study of the effect of trichostatin A (TSA), an inhibitor of histone deacetylase, on lipopolysaccharide (LPS)-induced expression of inducible nitric oxide synthase (iNOS) in RAW 264.7 macrophages and its underlying mechanisms. TSA pretreatment potently diminishes LPS-stimulated nitric oxide (NO) release and both mRNA and protein levels of iNOS in macrophages. The effects of TSA and LPS on transcription factors binding to two LPS-responsive elements within the iNOS promoter, one binding the NF-B site and the other the octamer element, were investigated. Results show that TSA did not alter the LPS-activated NF-B activity demonstrated by the nuclear translocation of p50 and p65 and by a NF-B-driven reporter gene expression system. In addition, neither TSA nor LPS changed the expression of Oct-1, a ubiquitously expressed octamer binding protein. However, TSA suppressed the LPS-induced expression of Oct-2, another octamer binding protein, at both mRNA and protein levels. Chromatin immunoprecipitation assays revealed that binding of Oct-2 to the iNOS promoter was enhanced by LPS treatment; however, pretreatment with TSA resulted in loss of this binding. Moreover, forced expression of Oct-2 by transfection of pCG-Oct-2 plasmid restored the TSA-suppressed iNOS expression elevated by LPS stimulation, further indicating that Oct-2 activation is a crucial step for transcriptional activation of the iNOS gene in response to LPS stimulation in macrophages. This study demonstrates that TSA diminishes iNOS expression in LPS-treated macrophages by inhibiting Oct-2 expression and thus reducing the production of NO. inducible nitric oxide synthase; lipopolysaccharide; macrophage HISTONE MODIFICATIONS, such as acetylation, methylation, and phosphorylation, play important roles in gene expression. Acetylation is the most frequent posttranslational modification of histone. The level of histone acetylation is controlled by the opposing activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) (11). Higher levels of histone acetylation lead to relaxation of the local structure of chromatin and thus increase the accessibility of transcription factors to the DNA, subsequently enhancing gene transcription. In contrast, silent genes are usually located at nucleosomes with hypoacetylated histone proteins (35).Trichostatin A (TSA) is a potent and nonselective inhibitor of HDACs and is widely used to study the role of histone acetylation in genes. TSA treatment induces histone acetylation in mammalian cells (25,38). However, several studies have shown that TSA treatment changes the expression of only about 2% of genes (24, 39). Aung et al. (1) reported that TSA enhanced LPS-induced expressions of Cox-2, Cxcl2, and Lfit2, whereas LPS-...

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“…POU2F1 also takes a part in regulation of cell type–specific gene expression. It regulates some genes in the immune system including those encoding light and heavy chains of immunoglobulins, IL‐2, IL‐3, IL‐5, IL‐8, granulocyte/macrophage colony stimulating factor, and CD20 15. Furthermore, an alternatively spliced variant of human POU2F1 is only expressed in lymphoid tissues and brain 15…”

Section: Discussionmentioning

confidence: 99%

Identification of hippocampus‐related candidate genes for Alzheimer's disease

Taguchi

Yamagata

Zhong

et al. 2005

Annals of Neurology

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Alzheimer's disease (AD) is a complex multifactorial disease in which many genetic and environmental factors are involved. We performed an association study using 376 AD patients and 376 control subjects. We studied 35 single nucleotide polymorphisms in 35 genes that were significantly downregulated or upregulated only in the AD hippocampus compared with control and found that 9 single nucleotide polymorphisms were associated with AD. Our data indicated that single nucleotide polymorphisms could highly reflect differences in gene expression. Furthermore, an intronic polymorphism (+9943T/C) in POU2F1 was most significantly associated with AD (p = 0.0007). Our results suggest that POU2F1 is a candidate gene for AD.

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Human Oct-1L isoform has tissue-specific expression pattern similar to Oct-2

Cited by 26 publications

References 25 publications

Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes

Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes

The essential role of Oct-2 in LPS-induced expression of iNOS in RAW 264.7 macrophages and its regulation by trichostatin A

Identification of hippocampus‐related candidate genes for Alzheimer's disease

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