Human nuclear respiratory factor 2? subunit cDNA: Isolation, subcloning, sequencing, and in situ hybridization of transcripts in normal and monocularly deprived macaque visual system

Guo, Aili; Nie, Feng; Wong‐Riley, Margaret T.T.

doi:10.1002/(sici)1096-9861(20000207)417:2<221::aid-cne7>3.0.co;2-4

Cited by 18 publications

(13 citation statements)

References 52 publications

(60 reference statements)

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“…Many genes under activity-dependent regulation have been reported (e.g.,, GAD67, Gamma-aminobutyric acid (GABA) receptors, CAMKs, NRF, zif268; Jones 1990; Benson et al 1991; Huntsman et al 1994; Chaudhuri et al 1995; Tighilet et al 1998; Nie and Wong-Riley 1999; Guo et al 2000). Nevertheless, the expression of these genes is neither lamina nor area specific (e.g., Okuno et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Enriched Expression of Serotonin 1B and 2A Receptor Genes in Macaque Visual Cortex and their Bidirectional Modulatory Effects on Neuronal Responses

et al. 2008

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To study the molecular mechanism how cortical areas are specialized in adult primates, we searched for area-specific genes in macaque monkeys and found striking enrichment of serotonin (5-hydroxytryptamine, 5-HT) 1B receptor mRNA, and to a lesser extent, of 5-HT2A receptor mRNA, in the primary visual area (V1). In situ hybridization analyses revealed that both mRNA species were highly concentrated in the geniculorecipient layers IVA and IVC, where they were coexpressed in the same neurons. Monocular inactivation by tetrodotoxin injection resulted in a strong and rapid (<3 h) downregulation of these mRNAs, suggesting the retinal activity dependency of their expression. Consistent with the high expression level in V1, clear modulatory effects of 5-HT1B and 5-HT2A receptor agonists on the responses of V1 neurons were observed in in vivo electrophysiological experiments. The modulatory effect of the 5-HT1B agonist was dependent on the firing rate of the recorded neurons: The effect tended to be facilitative for neurons with a high firing rate, and suppressive for those with a low firing rate. The 5-HT2A agonist showed opposite effects. These results suggest that this serotonergic system controls the visual response in V1 for optimization of information processing toward the incoming visual inputs.

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Section: Discussionmentioning

confidence: 99%

Enriched Expression of Serotonin 1B and 2A Receptor Genes in Macaque Visual Cortex and their Bidirectional Modulatory Effects on Neuronal Responses

et al. 2008

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“…The putative mitochondrial targetting sequence and the presence of a NRF-2 site indicate that the gene product is presumably a mitochondrial protein. NRF-2 has been shown to contribute to the transcriptional regulation of a number of nuclear genes that code for subunits of respiratory chain complexes, including cytochrome c oxidase, and for other mitochondrial proteins, such as the β-subunit of ATP synthase and the mitochondrial transcription factor (Virbasius et al 1993;Guo et al 2000).…”

Section: Discussionmentioning

confidence: 99%

CIA30 complex I assembly factor: a candidate for human complex I deficiency?

et al. 2002

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The human mitochondrial NADH:ubiquinone oxidoreductase (complex I), the first complex of the oxidative phosphorylation system, is composed of at least 42 subunits. Little is known about the assembly process of these subunits into the mature complex. Recently, two proteins in Neurospora crassa have been found to be involved in the assembly of complex I. These proteins are not constituent parts of the mature complex but are associated with smaller intermediate complexes of the assembly process and have a chaperone-like function. We have characterized the human homologue of one of these two complex I intermediate associated proteins, named CIA30, and show that expression of the human CIA30 protein is ubiquitous with a slightly higher expression in various heart tissues, kidney, lung and liver. As deletion of the Neurospora crassa CIA genes results in severe disruption of the assembly process, human CIA30 can be considered as a candidate gene related to complex I deficiency. Thirteen patients with an isolated complex I deficiency, but who were ruled out for mutations in the 35 nuclear genes of the complex and mtDNA, were subjected to mutational analysis of the gene coding for the human CIA30 protein. Four new single nucleotide polymorphisms (SNPs) were detected but no functional mutation was found.

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“…NRF-2 is also more strongly expressed in cell types that have higher COX activity than those with lower activity (Wong-Riley et al 2005). Moreover, NRF-2 itself responds to monocular impulse blockade by downregulating its protein and message levels in deprived cortical columns and neurons in which COX activity is suppressed (Nie and Wong-Riley 1999; Guo et al 2000; Wong-Riley et al 2005). In response to KCl depolarization in cultured primary neurons, NRF-2 protein is upregulated prior to the upregulation of COX subunit message and activity (Zhang and Wong-Riley 2000b), and both α and β subunits of NRF-2 respond to increased neuronal activity by translocating from the cytoplasm to the nucleus, where they associate primarily with euchromatin to activate their target genes (Yang et al 2004).…”

Section: 5 Transcription Factors As Bigenomic Coordinatorsmentioning

confidence: 99%

Bigenomic Regulation of Cytochrome c Oxidase in Neurons and the Tight Coupling Between Neuronal Activity and Energy Metabolism

Wong‐Riley

2012

Advances in Experimental Medicine and Biology

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Cytochrome c oxidase is the terminal enzyme of the mitochondrial electron transport chain, without which oxidative metabolism cannot be carried to completion. It is one of only four unique, bigenomic proteins in mammalian cells. The holoenzyme is made up of three mitochondrial-encoded and ten nuclear-encoded subunits in a 1:1 stoichiometry. The ten nuclear subunit genes are located in nine different chromosomes. The coordinated regulation of such a multisubunit, multichromosomal, bigenomic enzyme poses a challenge. It is especially so for neurons, whose mitochondria are widely distributed in extensive dendritic and axonal processes, resulting in the separation of the mitochondria from the nuclear genome by great distances. Neuronal activity dictates COX activity that reflects protein amount, which, in turn, is regulated at the transcriptional level. All 13 COX transcripts are up- and downregulated by neuronal activity. The ten nuclear COX transcripts and those for Tfam and Tfbms important for mitochondrial COX transcripts are transcribed in the same transcription factory. Bigenomic regulation of all 13 transcripts is mediated by nuclear respiratory factors 1 and 2 (NRF-1 and NRF-2). NRF-1, in addition, also regulates critical neurochemicals of glutamatergic synaptic transmission, thereby ensuring the tight coupling of energy metabolism and neuronal activity at the molecular level in neurons.

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Human nuclear respiratory factor 2? subunit cDNA: Isolation, subcloning, sequencing, and in situ hybridization of transcripts in normal and monocularly deprived macaque visual system

Cited by 18 publications

References 52 publications

Enriched Expression of Serotonin 1B and 2A Receptor Genes in Macaque Visual Cortex and their Bidirectional Modulatory Effects on Neuronal Responses

Enriched Expression of Serotonin 1B and 2A Receptor Genes in Macaque Visual Cortex and their Bidirectional Modulatory Effects on Neuronal Responses

CIA30 complex I assembly factor: a candidate for human complex I deficiency?

Bigenomic Regulation of Cytochrome c Oxidase in Neurons and the Tight Coupling Between Neuronal Activity and Energy Metabolism

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