Human nicotinic receptors in chromaffin cells: characterization and pharmacology

Albillos, Almudena; McIntosh, J. Michael

doi:10.1007/s00424-017-2073-0

Cited by 10 publications

(9 citation statements)

References 52 publications

(70 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with a dominant implication of nAChRs in catecholamine secretion, nicotinic receptors robustly contribute to post‐synaptic excitatory events, as reported ex vivo in acute slices in rat and in vivo in mouse . Adrenal chromaffin cells express various nAChR subtypes, including α3‐, α6‐, α7‐, and α9/α10‐built receptors, depending on species (see References for recent reviews). Each nAChR subtype can individually contribute to post‐synaptic events as long as it is located at the synapse.…”

Section: Introductionsupporting

confidence: 58%

Cholinergic and peptidergic neurotransmission in the adrenal medulla: A dynamic control of stimulus‐secretion coupling

Guérineau

2019

IUBMB Life

View full text Add to dashboard Cite

Synaptic neurotransmission at the splanchnic nerve‐chromaffin cell synapse is a chief element of the stimulus‐secretion coupling in the adrenal medullary tissue, managing and regulating the secretion of catecholamines. Making the state of play more intricate than initially envisioned, the synaptic vesicles of nerve terminals innervating the medulla contain various compounds, including various neurotransmitters and neuropeptides. Under basal conditions associated with a low splanchnic nerve discharge rate, neurotransmission is ensured by the synaptic release of the primary neurotransmitter acetylcholine (ACh). Under sustained and repetitive stimulations of the splanchnic nerve, as triggered in response to stressors, the synaptic release of neuropeptides, such as the pituitary adenylate cyclase‐activating polypeptide PACAP, supplants ACh release. The anatomical and functional changes that occur presynaptically at the preganglionic splanchnic nerve, combined with changes occurring postsynaptically at nicotinic acetylcholine receptors (nAChRs), confer the adrenomedullary synapses a solid and persistent aptitude to functional remodeling, from birth to aging. The present review focuses on the composite cholinergic and noncholinergic nature of neurotransmission occurring at the splanchnic nerve‐chromaffin cell synapse and its remodeling in response to physiological or pathological stimuli.

show abstract

Section: Introductionsupporting

confidence: 58%

Cholinergic and peptidergic neurotransmission in the adrenal medulla: A dynamic control of stimulus‐secretion coupling

Guérineau

2019

IUBMB Life

View full text Add to dashboard Cite

show abstract

“…Metabolomics analyses were conducted for stimulated secretion of neurotransmitters induced by nicotine − and high KCl depolarization. , These secretion studies also examined the effects of lithium related to regulation of catecholamines of bipolar disorder. − …”

Section: Resultsmentioning

confidence: 99%

Metabolomics Analyses of 14 Classical Neurotransmitters by GC-TOF with LC-MS Illustrates Secretion of 9 Cell–Cell Signaling Molecules from Sympathoadrenal Chromaffin Cells in the Presence of Lithium

Hook

Kind

Podvin

et al. 2019

ACS Chem. Neurosci.

View full text Add to dashboard Cite

The classical small molecule neurotransmitters are essential for cell-cell signaling in the nervous system for regulation of behaviors and physiological functions. Metabolomics approaches are ideal for quantitative analyses of neurotransmitter profiles, but have not yet been achieved for the repertoire of 14 classical neurotransmitters. Therefore, this study developed targeted metabolomics analyses by full scan gas chromatography / time of flight mass spectrometry (GC-TOF) and hydrophilic interaction chromatography-QTRAP mass spectrometry (HILIC-MS/MS) operated in positive ionization mode for identification and quantitation of 14 neurotransmitters consisting of acetylcholine, adenosine, anandamide, aspartate, dopamine, epinephrine, GABA, glutamate, glycine, histamine, melatonin, norepinephrine, serine, and serotonin. GC-TOF represents a new metabolomics method for neurotransmitter analyses. Sensitive measurements of 11 neurotransmitters were achieved by GC-TOF, and three neurotransmitters were analyzed by LC-MS/MS (acetylcholine, anandamide, and melatonin). The limits of detection (LOD) and limits of quantitation (LOQ) were assessed for linearity for GC-TOF and LC-MS/MS protocols. In neurotransmitter-containing dense core secretory vesicles of adrenal medulla, known as chromaffin granules (CG), metabolomics measured the concentrations of 9 neurotransmitters consisting of the catecholamines dopamine, norepinephrine, and epinephrine, combined with glutamate, serotonin, adenosine, aspartate, glycine, and serine. The CG neurotransmitters were constitutively secreted from sympatho-adrenal chromaffin cells in culture. Nicotine-and KClstimulated release of the catecholamines and adenosine. Lithium, a drug used for the treatment of

show abstract

“…Nicotinic ACh receptor (nAChR), as an ionotropic receptor, was widely reported to be involved in catecholamine secretion and synthesis [ 21 ]. Because of its important role in regulation of the nerve system and involvement in a variety of diseases, nAChR was well investigated for decades from its structure to the physical functions.…”

Section: Discussionmentioning

confidence: 99%

The Inhibition Effects of Shenmai Injection on Acetylcholine‐Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells

Zhang

Wang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Shenmai injection (SMI) has been widely used for the treatment of cardiovascular diseases in China. Cardiovascular disorders are often related to excessive catecholamine (CA) secretion. Here, we report the effects of SMI on CA secretion and synthesis in cultured bovine adrenal medullary cells. We found that SMI significantly reduced CA secretion induced by 300 μM acetylcholine (ACh). Cotreatment with SMI (10 μL/mL) and either of the ACh receptor α-subunit inhibitors, HEX (α3) or DhβE (α4β2), did not produce any further inhibition, indicating that SMI may play a role through α3 and α4β2 channels. Furthermore, SMI reduced tyrosine hydroxylase (TH) activity induced by ACh by inhibiting the phosphorylation of TH at Ser19 and Ser40. TH is phosphorylated at Ser19 by Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) and at Ser40 by protein kinase A (PKA). KN-93 and H89, the antagonists of CaM kinase II and PKA, respectively, inhibited the ACh-induced phosphorylation at Ser19 and Ser40, and the addition of SMI did not augment the inhibitory effect. Taken together, our results show that SMI likely inhibits CA secretion by blocking TH activity at its Ser19 and Ser40 sites.

show abstract

Human nicotinic receptors in chromaffin cells: characterization and pharmacology

Cited by 10 publications

References 52 publications

Cholinergic and peptidergic neurotransmission in the adrenal medulla: A dynamic control of stimulus‐secretion coupling

Cholinergic and peptidergic neurotransmission in the adrenal medulla: A dynamic control of stimulus‐secretion coupling

Metabolomics Analyses of 14 Classical Neurotransmitters by GC-TOF with LC-MS Illustrates Secretion of 9 Cell–Cell Signaling Molecules from Sympathoadrenal Chromaffin Cells in the Presence of Lithium

The Inhibition Effects of Shenmai Injection on Acetylcholine‐Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells

Contact Info

Product

Resources

About