Human neutrophil azurocidin synergizes with leukocyte elastase and cathepsin G in the killing of Capnocytophaga sputigena

Miyasaki, Kenneth T.; Bodeau, A L

doi:10.1128/iai.60.11.4973-4975.1992

Cited by 24 publications

(3 citation statements)

References 17 publications

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“…ACPP, a non-specific tyrosine phosphatase, is well-described to be associated with prostate cancer (56), and is used clinically as a diagnostic marker. AZU1, an antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein, which acts in conjunction with cathepsin G in host-defense mechanisms (57), was hypothesized to be a potential pancreatic cancer biomarker in the pancreatic juice (58). Histones H1 have been reported to be involved in the survival of breast cancer cells (59), and H1.2 specifically was identified as an apoptogenic factor (60).…”

Section: Discussionmentioning

confidence: 99%

Identification of diagnostic upper gastrointestinal cancer tissue type‑specific urinary biomarkers

et al. 2019

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Several potential urinary biomarkers exhibiting an association with upper gastrointestinal tumour growth have been previously identified, of which S100A6, S100A9, rabenosyn-5 and programmed cell death 6-interacting protein (PDCD6IP) were further validated and found to be upregulated in malignant tumours. The cancer cohort from our previous study was subclassified to assess whether distinct molecular markers can be identified for each individual cancer type using a similar approach. Urine samples from patients with cancers of the stomach, oesophagus, oesophagogastric junction or pancreas were analysed by surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry using both CM10 and IMAC30 (Cu2+-complexed) chip types and LC-MS/MS-based mass spectrometry after chromatographic enrichment. This was followed by protein identification, pattern matching and validation by western blotting. We found 8 m/z peaks with statistical significance for the four cancer types investigated, of which m/z 2447 and 2577 were identified by pattern matching as fragments of cathepsin-B (CTSB) and cystatin-B (CSTB); both molecules are indicative of pancreatic cancer. Additionally, we observed a potential association of upregulated α-1-antichymotrypsin with pancreatic and gastric cancers, of PDCD6IP, vitelline membrane outer layer protein 1 homolog (VMO1) and triosephosphate isomerase (TPI1) with oesophagogastric junctional cancers, and of complement C4-A, prostatic acid phosphatase, azurocidin and histone-H1 with oesophageal cancer. Furthermore, the potential pancreatic cancer biomarkers CSTB and CTSB were validated independently by western blotting. Therefore, the present study identified two new potential urinary biomarkers that appear to be associated with pancreatic cancer. This may provide a simple, non-invasive screening test for use in the clinical setting.

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Section: Discussionmentioning

confidence: 99%

Identification of diagnostic upper gastrointestinal cancer tissue type‑specific urinary biomarkers

et al. 2019

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“…Nonetheless, a helper role has been attributed to elastase, which appears to potentiate the cidal effect of other active proteins. This has been observed to be the case for the killing of C. sputigena, where very high concentrations of azurocidin become cidal when combined with elastase (18). Interestingly, the interaction between these two granule proteins was found to be enzyme dependent.…”

Section: Discussionmentioning

confidence: 81%

Elastase Is the Only Human Neutrophil Granule Protein That Alone Is Responsible for In Vitro Killing of Borrelia burgdorferi

García

Gusmani²,

Murgia

et al. 1998

Infect Immun

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Phagocytosis of Borrelia burgdorferi by human polymorphonuclear leukocytes triggers oxygen-dependent and -independent mechanisms of potentially cidal outcome. Nevertheless, no factor or process has yet been singled out as being borreliacidal. We have studied the B. burgdorferi-killing ability of the myeloperoxidase-H2O2-chloride system and that of primary and secondary granule components in an in vitro assay. We found that neither secondary granule acid extracts nor the chlorinating system could kill these microorganisms, while primary granule extracts were effective. The Borrelia-killing factor was purified to homogeneity and demonstrated to be elastase. Its cidal activity was found to be independent of its proteolytic activity.

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“…A double-loop mutant of Azu in which all eight basic residues were replaced with glutamines demonstrates decreased ability to bind heparin and to kill E. coli and C. albicans [84]. Azu acts in synergy with NE and CatG to kill Capnocytophaga sputigena [85] and has opsonic activity toward S. aureus [86].…”

Section: Serprocidinsmentioning

confidence: 99%

Antimicrobial proteins and peptides: anti-infective molecules of mammalian leukocytes

Levy

2004

Journal of Leukocyte Biology

161

105

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Phagocytic leukocytes are a central cellular element of innate-immune defense in mammals. Over the past few decades, substantial progress has been made in defining the means by which phagocytes kill and dispose of microbes. In addition to the generation of toxic oxygen radicals and nitric oxide, leukocytes deploy a broad array of antimicrobial proteins and peptides (APP). The majority of APP includes cationic, granule-associated (poly)peptides with affinity for components of the negatively charged microbial cell wall. Over the past few years, the range of cells expressing APP and the potential roles of these agents have further expanded. Recent advances include the discovery of two novel families of mammalian APP (peptidoglycan recognition proteins and neutrophil gelatinase-associated lipocalin), that the oxygen-dependent and oxygen-independent systems are inextricably linked, that APP can be deployed in the context of novel subcellular organelles, and APP and the Toll-like receptor system interact. From a clinical perspective, congeners of several of the APP have been developed as potential therapeutic agents and have entered clinical trials with some evidence of benefit.

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Human neutrophil azurocidin synergizes with leukocyte elastase and cathepsin G in the killing of Capnocytophaga sputigena

Cited by 24 publications

References 17 publications

Identification of diagnostic upper gastrointestinal cancer tissue type‑specific urinary biomarkers

Identification of diagnostic upper gastrointestinal cancer tissue type‑specific urinary biomarkers

Elastase Is the Only Human Neutrophil Granule Protein That Alone Is Responsible for In Vitro Killing of Borrelia burgdorferi

Antimicrobial proteins and peptides: anti-infective molecules of mammalian leukocytes

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