Human neuropeptide Y, somatostatin and vasopressin precursors identified in cell‐free translations of hypothalamic mRNA

Ivell, Richard; Emson, Piers C.; Richter, Dietmar

doi:10.1016/0014-5793(84)81278-8

Cited by 15 publications

(3 citation statements)

References 18 publications

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“…Cell-free translation studies using human hypothalamic poly(A)+ RNA, however, indicated that the size of the prepro-vasopressin precursor was similar to that reported from rat and cow (7). This was in agreement with protein data which suggested that high-molecular-mass material with vasopressin-and neurophysin-like structure was present in extracts from human pituitary [S] and lung carcinoma [9].…”

Section: Introductionsupporting

confidence: 80%

“…Transiation products were separated on SDS-polyacrylamide gels and subjected to fluorography [7]. a, b, molecular mass markers (30 and 14 kDa, respectively).…”

Section: Sequence and Organization Of The Hormonementioning

confidence: 99%

“…Poly(A)+ RNA was translated in a rabbit reticulocyte lysate in vitro system, specific translation products were immunoprecipitated and isolated as described [7].…”

Section: Cell-free Translationmentioning

confidence: 99%

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Expression of the vasopressin and oxytocin genes in human hypothalami

et al. 1985

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Poly(A)+ RNA isolated from post‐mortem human hypothalami has been used to characterize the polyprotein precursors to vasopressin and oxytocin. Translation in a cell‐free system and subsequent immunoprecipitation with antibodies raised against either vasopressin or neurophysin identified a product of M r 19000 (prepro‐vasopressin). A second less intense product of M r 16500 was tentatively identified as prepro‐oxytocin. A cDNA library derived from the human hypothalamic poly(A)+ RNA was screened for vasopressin and oxytocin‐encoding cDNA using heterologous probes; clones encoding the two precursors were identified and found to be organized as their rat and bovine counterparts. Northern blot analysis shows that the mRNAs for the two prepro‐hormones consist of ~ 840 (AVP) and ~ 700 (OT) nucleotides.

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Section: Introductionsupporting

confidence: 80%

“…Transiation products were separated on SDS-polyacrylamide gels and subjected to fluorography [7]. a, b, molecular mass markers (30 and 14 kDa, respectively).…”

Section: Sequence and Organization Of The Hormonementioning

confidence: 99%

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Expression of the vasopressin and oxytocin genes in human hypothalami

et al. 1985

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Pancreatic Polypeptide Family: Pancreatic Polypeptide, Neuropeptide Y, and Peptide YY

Taylor

1989

Comprehensive Physiology

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Localization and regulation of vasopressin mRNA in human neurons

Rivkees¹,

Chaar

Hanley

et al. 1989

Synapse

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Vasopressin (prepropressophysin) mRNA is detected in neurons of the supraoptic, suprachiasmatic, and paraventricular nuclei of human postmortem hypothalamic specimens by quantitated in situ hybridization using 35S-labeled single-stranded cDNA probes directed against exon C of the human vasopressin gene. This hybridization displays the anticipated anatomic distribution, as well as several biochemical features supporting its specificity. Hybridization densities in supraoptic neurons, a measure of vasopressin gene expression, display substantial variability from brain-to-brain. We can attribute much of this brain-to-brain variability to differences in antemortem extracellular volume status. This conclusion is based on a) animal models of the human postmortem process, b) animal models of common agonal events, c) good correlations between antemortem volume status and neuronal vasopressin mRNA hybridization densities in human postmortem specimens matched for age and postmortem interval, and d) our inability to correlate human neuronal vasopressin mRNA hybridization densities with other clinical and postmortem features. These results provide an example of antemortem regulation of a human neuroendocrine gene using postmortem tissue.

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Human neuropeptide Y, somatostatin and vasopressin precursors identified in cell‐free translations of hypothalamic mRNA

Abstract: Messenger mRNA has been prepared from post mortem human hypothalami and translated in a cell-free system. Using specific antibodies, biosynthetic precursors have been identified to neuropeptide Y (12 kDa), somatostatin (15 kDa) and vasopressin/neurophysin (19 kDa).

Cited by 15 publications

References 18 publications

Expression of the vasopressin and oxytocin genes in human hypothalami

Expression of the vasopressin and oxytocin genes in human hypothalami

Pancreatic Polypeptide Family: Pancreatic Polypeptide, Neuropeptide Y, and Peptide YY

Localization and regulation of vasopressin mRNA in human neurons

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