Human muscle neural cell adhesion molecule (N-CAM): Identification of a muscle-specific sequence in the extracellular domain

Dickson, George; Gower, Hilary J.; Barton, Chris; Prentice, Howard; Elsom, Vicki L.; Moore, Stephen E.; Cox, Roger; Quinn, Christopher A.; Putt, Wendy; Walsh, Frank S.

doi:10.1016/0092-8674(87)90178-4

Cited by 208 publications

(103 citation statements)

References 42 publications

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“…One exception to this proposal is the interface between the 2 FnIII domains, as deletion of Fn2 did not affect binding. The Fn1 Fn2 boundary is the site of several splice variants, which range from the introduction of a single aag codon to the additional 35 amino acids found in the musclespecific domain (MSD) (39). The existence of these splice variants, and the current finding that Fn2 is not required for bead aggregation, argues that the domain-domain interactions between Fn1 and Fn2 are not required for maximal homophilic binding.…”

Section: Discussionmentioning

confidence: 99%

Neural Cell Adhesion Molecule (N-CAM) Homophilic Binding Mediated by the Two N-terminal Ig Domains Is Influenced by Intramolecular Domain-Domain Interactions

Atkins¹,

Gallin

Owens

et al. 2004

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

Neural Cell Adhesion Molecule (N-CAM) Homophilic Binding Mediated by the Two N-terminal Ig Domains Is Influenced by Intramolecular Domain-Domain Interactions

Atkins¹,

Gallin

Owens

et al. 2004

Journal of Biological Chemistry

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“…There are five different NCAM mRNA size classes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb in adult rat brain [6]. More than 20 different exons are described that code for the distinct NCAM polypeptides [5,[7][8][9][10][11]. In the genome several small exons exist…”

Section: Introductionmentioning

confidence: 99%

Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes

et al. 1990

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Neural cell adhesion molecule; Northern blotting; Oligonucleotide A number of different isoforms of the neural cell adhesion molecule (NCAM) have been identified. The difference between these is due to alternative splicing of a single NCAM gene. In rat brain NCAM mRNAs with sizes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb have been reported. We have synthesized six DNA oligonucleotides, that hybridize to different exons in the NCAM gene. Furthermore we have constructed three oligonucleotides, that exclusively hybridize to mRNAs lacking certain exons, by letting them consist of sequences adjacent to both sides of the splice sites. By means of these probes we have characterized the five NCAM mRNAs in rat brain.

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“…First the prospective myogenic stem cells are initiated along the myogenic pathway in a process found in the extracellular domain of some NCAM isoforms. Downstream of the 5 immunoglobulin domains a set of alternatively spliced exons has been identified and called the MSDl region reflecting the fact that the initial studies were performed on skeletal muscle (Dickson et al, 1987;Walsh and Doherty, 1991). These exons encode a 37 amino acid insert found in the GPI anchored NCAM isoforms in myotubes.…”

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confidence: 99%

Expression of NCAM isoforms during skeletal myogenesis in the mouse embryo

Lyons

Moore

Yahara

et al. 1992

Developmental Dynamics

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We have examined the developmental patterns of neural cell adhesion molecule (NCAM) gene expression in embryonic mouse skeletal muscle cells by in situ hybridization. Moreover, by utilising exon-specific cRNA probes, we have examined tissue specific splicing of the NCAM gene. We show that there is a distinct sequence of NCAM isoform expression during skeletal muscle development. Since NCAMs are also expressed in other cell types, particularly neurons, NCAM mRNAs have been colocalised with acetylcholine receptor a (AChRa) gene transcripts to identify muscle-specific expression. NCAM is first detected in somites as they first form, prior to their differentiation into muscle and nonmuscle compartments. Myotomes, the first skeletal muscle masses to form in the embryo, express mRNAs for the transmembrane 180 and 140 kDa isoforms of NCAM. Both of these transcripts are also detected in the neural tube, and their spatial pattern of expression changes with development. Transcripts containing the muscle-specific domain (MSD) of the NCAM gene are not detected prior to 11 days postcoitum (p.c.1, at a time when rostra1 somites already contain well-developed myotomes. As the level of MSD mRNAs increases at 12 days P.c., the 140 and 180 kDa transcript levels decrease in skeletal muscle masses. The level of all NCAM isoform transcripts declines between 13 and 15 days p.c. in muscle. However, the 180 and 140 kDa NCAM isoforms are expressed at a high level in neural tissue and in other locations in the developing embryo such as in smooth muscle, around vibrissae follicles, and in the perichondrial zone of digits. o 1992 Wiley-Liss, Inc.

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Human muscle neural cell adhesion molecule (N-CAM): Identification of a muscle-specific sequence in the extracellular domain

Cited by 208 publications

References 42 publications

Neural Cell Adhesion Molecule (N-CAM) Homophilic Binding Mediated by the Two N-terminal Ig Domains Is Influenced by Intramolecular Domain-Domain Interactions

Neural Cell Adhesion Molecule (N-CAM) Homophilic Binding Mediated by the Two N-terminal Ig Domains Is Influenced by Intramolecular Domain-Domain Interactions

Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes

Expression of NCAM isoforms during skeletal myogenesis in the mouse embryo

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