Human Monocytes and Lymphocytes Produce Different Mixtures of α-Interferon Subtypes

Goren, Tamar; Fischer, Dina G.; Rubinstein, Menachem

doi:10.1089/jir.1986.6.323

Cited by 21 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, there are large differences in antiviral activities obtained with various cell lines. Goren et al have shown that when subtypes were assayed in bovine MDBK cells (on which IFN-α activity is measured by manufacturer), specific activities were found to be high and in a narrow range compared with human cells (15). Our data show that, with the same concentration (10 IU/ml), various IFN-α subtypes are not equally effective in inducing MxA.…”

Section: Discussionmentioning

confidence: 49%

Correlation between the Anti‐Virus‐Induced Cytopathic Effect Activity of Interferon‐α Subtypes and Induction of MxA Protein In Vitro

Schanen

Chieux

Lobert

et al. 2006

Microbiology and Immunology

View full text Add to dashboard Cite

There are several interferon‐α (IFN‐α) subtypes. Mechanism of disparity in biological effects among members of IFN‐α subtypes remains unexplained. Biological activity of IFN‐α is mediated in part by induction of intracellular antiviral proteins. We studied whether differences in biologic effects of IFN‐α subtypes may rely on their antiviral protein inducing effect. Intracellular induction of MxA protein and anti‐virus‐induced cytopathic effect (CPE) activity of 11 IFN‐α subtypes in human amnion WISH cells have been studied. MxA protein quantitation in cell lysates was performed by immunochemiluminescence assay and anti‐virus‐induced CPE activity was assessed by protection against vesicular stomatitis virus (VSV)‐induced CPE. Range of MxA values was high when cells were treated with 10 and 100 IU/ml of each IFN‐α subtype. Levels of MxA correlated with anti‐VSV‐induced CPE obtained with 10 IU/ml IFN‐α subtype. Together our data show a disparity in MxA‐inducing activity of IFN‐α subtypes and suggest that differences in anti‐VSV‐induced CPE of IFN‐α subtypes in WISH cells can be related to their different ability to induce MxA.

show abstract

Section: Discussionmentioning

confidence: 49%

Correlation between the Anti‐Virus‐Induced Cytopathic Effect Activity of Interferon‐α Subtypes and Induction of MxA Protein In Vitro

Schanen

Chieux

Lobert

et al. 2006

Microbiology and Immunology

View full text Add to dashboard Cite

show abstract

“…Monocytes-macrophages produce IFN-cx under certain stimulatory conditions (15,20). Several studies have also been reported on the induction of IFN-tx by HIV-1 or the rgpl2O protein in human monocytes cultured in the presence of macrophage colony-stimulating factor or in peripheral blood mononuclear cells (3,8,11,13,25).…”

Section: U-2bmentioning

confidence: 99%

Induction of beta interferon by human immunodeficiency virus type 1 and its gp120 protein in human monocytes-macrophages: role of beta interferon in restriction of virus replication

Gessani

Puddu

Varano

et al. 1994

J Virol

View full text Add to dashboard Cite

In vitro cultivated human monocytes show a time-dependent differentiation into macrophages, characterized by an increased expression of macrophage-specific antigens. Monocytes-macrophages were infected with human immunodeficiency virus type 1 strain Ba-L (H1V-lBaL) at different stages of differentiation. When 7-day cultured macrophages were infected in the presence of antibodies to beta interferon (IFN-,B), a significant increase in HIV-1 p24 release was detected. This effect was not observed in 1-day monocytes. This finding suggests that IFN-, secreted by the infected macrophages inhibits p24 release. Treatment of cultured macrophages with recombinant gpl20 (rgpl20) protein resulted in the induction of IFN-13 mRNA and in an antiviral state to vesicular stomatitis virus. This rgpl20-induced antiviral state was largely neutralized by antibodies to IFN-13, whereas anti-IFN-a antibodies were ineffective. In cultured macrophages, 0.1 IU of IFN-P per ml was sufficient to induce a marked inhibition of vesicular stomatitis virus yield, whereas this dose was ineffective in 1-day monocytes. These results indicate that (i) HIV-1 (possibly in part through its gpl20 protein) induces low levels of IFN-0 in macrophages and (ii) this IFN-, is very effective in inducing an antiviral state in differentiated macrophages.

show abstract

Post‐transcriptional regulation of interferon‐alpha 4 subtype production by lymphoblastoid cells

Greenway

Brandt

Hertzog

et al. 1993

Hematological Oncology

View full text Add to dashboard Cite

The constitutive production of interferon-alpha (IFN-alpha) subtypes by the lymphoblastoid cell lines, Namalwa, Daudi and Raji, was investigated using sensitive and semi-quantitative flow cytometric techniques. Further, we sought to determine whether the previously described failure of these cell lines to produce IFN-alpha-4 was a result of the deletion of the IFN A4 gene. Cytoplasmic production of IFN-alpha-2 and IFN-alpha-4 was assessed using IFN-alpha subtype-specific antipeptide antibodies and FITC-labelled secondary antibodies in indirect immunofluorescence-flow cytometry studies. The constitutive production of IFN-alpha-2 was detected in all three cell lines. Significant increases in fluorescence representing increased production of IFN-alpha-2 and possibly other IFN-alpha subtypes were detected after induction by Sendai virus. Approximately 100 per cent of cells in the Namalwa, Daudi and Raji cell populations contained IFN-alpha-2 before and after induction. However, no cells from the same cell populations contained the IFN-alpha-4 subtype. Analysis of genomic DNA isolated from the lymphoblastoid cells using the Polymerase Chain Reaction (PCR) and oligonucleotide primers specific for IFN A2 or IFN A4 confirmed the presence of the genes encoding both IFN-alpha subtypes. Furthermore, using reverse transcriptase-PCR amplification, mRNAs for both IFN-alpha-2 and IFN-alpha-4 were detected. Therefore, in contrast to some leukaemias and derived cell lines where IFN A genes have been deleted, these cell lines of B cell lineage exhibit selective expression of IFN A genes, as a result of altered transcriptional/translational control of IFN-alpha expression.

show abstract

Human Monocytes and Lymphocytes Produce Different Mixtures of α-Interferon Subtypes

Cited by 21 publications

References 15 publications

Correlation between the Anti‐Virus‐Induced Cytopathic Effect Activity of Interferon‐α Subtypes and Induction of MxA Protein In Vitro

Correlation between the Anti‐Virus‐Induced Cytopathic Effect Activity of Interferon‐α Subtypes and Induction of MxA Protein In Vitro

Induction of beta interferon by human immunodeficiency virus type 1 and its gp120 protein in human monocytes-macrophages: role of beta interferon in restriction of virus replication

Post‐transcriptional regulation of interferon‐alpha 4 subtype production by lymphoblastoid cells

Contact Info

Product

Resources

About