Human Monocyte Regulation of Connective Tissue Growth

DeLustro, Frank; Mackel, A M; DeLustro, Barbara; LeRoy, E. Carwile

doi:10.1093/icb/23.1.213

Cited by 12 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lack of dedifferentiation of a large number of cells may also derive from the influence of the numerous inflammatory macrophages present in the limb over the fibroblasts released on the stump surface that, like during mammalian healings (De Lustro et al . ; Wynn ), stimulate scarring. It is therefore suggested that after the initial 7–15 days or longer needed for covering the stump and accumulate mesenchymal cells/fibroblasts in the initial blastema, these cells become less proliferating and more differentiated towards the production of high levels of collagen.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies also indicated that most of the long retaining cells (cells that do not/little dilute injected 5BrdU over 1-2 months), potential progenitor/stem cells of the limbs, are mainly represented by fibrocytes located in the dermis and intermuscle connectives, so that the latter are the likely main source of cells for the initial formation of a limb blastema (Alibardi, unpublished observations). The lack of dedifferentiation of a large number of cells may also derive from the influence of the numerous inflammatory macrophages present in the limb over the fibroblasts released on the stump surface that, like during mammalian healings (De Lustro et al 1983;Wynn 2008), stimulate scarring. It is therefore suggested that after the initial 7-15 days or longer needed for covering the stump and accumulate mesenchymal cells/fibroblasts in the initial blastema, these cells become less proliferating and more differentiated towards the production of high levels of collagen.…”

Section: Various Factors Influence the Different Regenerative Responsmentioning

confidence: 99%

See 1 more Smart Citation

Cell proliferation in the amputated limb of lizard leading to scarring is reduced compared to the regenerating tail

Alibardi

2016

Acta Zoologica

View full text Add to dashboard Cite

After amputation, the tail of lizards regenerates while the limb forms a short scarring outgrowth. Using phospho‐histone‐H3 immunohistochemistry the mitotic activity of limb tissues at 12–25 days after amputation has been studied, when a limb outgrowth of 0.5–2 mm in length is covered by wound epidermis and the underlying connective is turning into a dense scar. In comparison with a regenerating tail of 3–5 mm in length, the number of dividing cells is reduced of 40–70% in different tissues of the scarring limb 1–2 mm in length at 18 days postamputation. Dividing cells are still present at 12–25 days postamputation in the cartilaginous epiphyses of the transected tibia and fibula and of the untransected femur. Also, the injured muscles present at the base of the scarring outgrowth still contain sparse dividing cells after 25 days postamputation of the limb. Together previous studies, the present observations suggest that after the initial proliferation of fibroblasts deriving from the injured tissues, especially from the dermis and intermuscle connectives during the initial 7–15 days postinjury, these cells cover the injured tissues underneath the wound epidermis, but rapidly produce high levels of collagen turning the initial blastema into a scar.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Various Factors Influence the Different Regenerative Responsmentioning

confidence: 99%

Cell proliferation in the amputated limb of lizard leading to scarring is reduced compared to the regenerating tail

Alibardi

2016

Acta Zoologica

View full text Add to dashboard Cite

show abstract

“…The intense damage of tissues in the arm versus the low tissue damage in tail tissues stimulates a persistent and massive debridment by macrophages in the limb. In chronic inflammatory reactions of mammalian tissues, macrophages through the secretion of FGF, PDGF, and TGF beta‐1 stimulate the recruitment and proliferation of fibroblasts capable to synthesize high amount of collagen and therefore induce scarring (DeLustro et al. 1983; Kovacs and DiPietro 1994; Ferguson and O’Kane 2004).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural features of the process of wound healing after tail and limb amputation in lizard

Alibardi

2010

Acta Zoologica

View full text Add to dashboard Cite

Alibardi, L. 2010. Ultrastructural features of the process of wound healing after tail and limb amputation in lizard.-Acta Zoologica (Stockholm) 91: 306-318Wound healing and re-epitelization after amputation of tail and limb in lizard have been studied by electron microscopy to understand the cytological base of immunity to infection in this species. After 2 days post-amputation in both limb and tail stumps, numerous granulocytes are accumulated over the stump, and participate to the formation of the scab. Bacteria remain confined to the scab or are engulfed by leukocytes and migrating keratinocytes located underneath the scab. Bacteria are degraded within lysosomes present in these cells and are not observed among mesenchymal cells or in blood vessels of the regenerative blastema. Granulocytes, migrating keratinocytes, and later macrophages form an effective barrier responsible for limiting microbe penetration. The innate immunity in lizard is very effective in natural (dirty) condition and impedes the spreading of infection to inner tissues. While the complete re-epitelization of the tail stump underneath the scab requires 4-7 days, the same process in the limb requires 8-18 or more days post-amputation, depending from the level of amputation and the persistence of a protruding humerus or femurs on the stump surface. This delay produces the permanence of inflammatory cells such as granulocytes and macrophages in the limb stump for a much longer period than in the tail stump, a process that stimulates scarring.

show abstract

“…Increase in the fibroblast population may then contribute to connective tissue formation. Enhanced growth offibroblasts and collagen production were promoted by blood monocytes via mediators (227) or by a factor released from human alveolar macrophages after stimulation by nonmineral particles (228). However, this macrophage-derived growth factor (MDGF) was unable by itselfto stimulate fibroblast replication, for which initiating factors provided by fibroblasts or platelets were required to establish competence.…”

Section: Macrophage/monocyte Participationmentioning

confidence: 99%