Human milk and infant formulae: Peptide differences and the opportunity to address the functional gap

Lopez, Cyril; Adelfio, Alessandro; Wall, Audrey; Molloy, Brendan; Holton, Thérèse A.; Khaldi, Nora

doi:10.1016/j.crfs.2020.07.003

Cited by 7 publications

(10 citation statements)

References 41 publications

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“…Additionally, many peptides derived from butyrophilin subfamily 1 member A1 (53) and polymeric immunoglobulin receptor (44). Further peptides originated from α S1 -casein (28), osteopontin (21), bile saltactivated lipase (20), perilipin-2 (19), perilipin-3 ( 14) and a few other proteins represented by less than ten peptides.…”

Section: Human Milkmentioning

confidence: 99%

“…The average lengths of β-casein-derived peptides slightly decreased from human (20.3) to goat (19) and further in cow milk samples (16.5) (Figure S5A). Similarly, the average length of α S1 -casein peptides decreased much from human (24.1) to goat (15.5) and cow milk samples (15.1) (Figure S5B).…”

Section: Common Source Proteinsmentioning

confidence: 99%

“…Human breast milk contains a variety of endogenous peptides, at least partially preformed in the mammary glands, with important biological functions [15][16][17]19,23]. The endogenous peptidomes of cow milk [24] and cow milk-based powdered IF have also been reported [13].…”

Section: Identification Of Endogenous Peptidesmentioning

confidence: 99%

“…As production regulations for IFs do not consider the peptidome, they will significantly differ from human milk. Only two studies have compared the peptidomes of human milk and IFs [18,19]. We hypothesized that the differences in the milk sources used for IF production and the different processing conditions applied to powders and ready-to-drink IFs affect the peptidomes of the final products and therefore the aim of this study was to qualitatively characterize the peptidomes of different types of IF and to identify similarities and differences compared to the peptidome of human milk.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the Endogenous Peptidomes of Different Infant Formula Types and Human Milk

Wölk

Gebauer

Hoffmann

et al. 2021

Foods

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Infant formula (IF) is a commonly used replacement whenever mother’s own milk is not available. Most IFs are based on cow milk (powders, liquids). Alternatives, based on other sources such as goat milk or plants, exist. Independent of the source, IF production and composition are strictly regulated. Besides proteins, minerals, and lipids, milk contains a variety of endogenous peptides. Whereas the human milk peptidome has been studied intensively, the peptidomes of IFs have been mostly neglected. This study investigated the peptidomes of different types of first stage IF, including cow milk-based powders and liquids, and powdered goat milk-based IF, highlighting major similarities and differences to human milk. Extracted native peptidomes were analyzed by nanoRPC-ESI-MS/MS using two different fragmentation techniques allowing the confident identification of 1587 peptides. β-Casein peptides dominated in all samples. Interestingly, powdered and liquid cow milk-based IFs differed in the numbers of β- and αS1-casein peptides, indicating processing-derived variations. However, the peptidomes of cow and goat milk-based IF appeared to be more comparable to each other than to human milk. Despite an overlap in the major source proteins, many peptide sequences were different, i.e., species-specific. Remarkably, the data indicate that the human milk peptidome might be donor-specific as well.

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Section: Human Milkmentioning

confidence: 99%

Section: Common Source Proteinsmentioning

confidence: 99%

Section: Identification Of Endogenous Peptidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of the Endogenous Peptidomes of Different Infant Formula Types and Human Milk

Wölk

Gebauer

Hoffmann

et al. 2021

Foods

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“…Egyptian Journal of Chemistry http://ejchem.journals.ekb.eg/ It is well known that human milk contains the necessary nutrients and basic required biologically active components that required for the early development of infant [11]. However, infants are the most subjected group for exposure to dl-PCBs [12].…”

Section: Introductionmentioning

confidence: 99%

A two years study of dioxin-like polychlorinated biphenyls (dl-PCBs) in mother's milk in Qalyubia governorate, Egypt.

et al. 2020

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The high cost of dl-PCBs analysis using a high-resolution gas chromatography/mass spectrometry (HR-GC/MS) generally minimizes it is regular analysis studies, especially in human tissue. In this study, a survey was carried out for analysis of dl-PCBs in thirty human milk samples collected from Qalyubia governorate, Egypt (2016)(2017)(2018). Significant correlations were observed between Ʃdl-PCBs (TEQ), age, and numbers of deliveries. All dl-PCBs congeners were detected in all collected samples. Besides, the non-ortho PCB congener (PCB 126) was found at elevated concentrations. The mean TEQ concentrations of non-ortho and mono-ortho PCBs in the breast milk of the primiparous mothers were 7.30 and 0.20 pgTEQg -1 lipid, respectively. On the other hand, multiparous mothers have a mean TEQ concentration for these dl-PCBs (non-ortho and mono-ortho PCBs) of 6.1 and 0.3 pg TEQ g -1 lipid, respectively. The most found dl-PCBs were PCB-118 and PCB-156, both account 64.9% from ƩPCBs concentrations without TEF. Estimation of infant daily intakes (EIDI) showed that they are at high risk of adverse effects caused by PCBs.

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Analysis of milk with liquid chromatography–mass spectrometry: a review

Imperiale

Morozova

Ferrentino

et al. 2023

Eur Food Res Technol

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As a widely consumed foodstuff, milk and dairy products are increasingly studied over the years. At the present time, milk profiling is used as a benchmark to assess the properties of milk. Modern biomolecular mass spectrometers have become invaluable to fully characterize the milk composition. This review reports the analysis of milk and its components using liquid chromatography coupled with mass spectrometry (LC–MS). LC–MS analysis as a whole will be discussed subdivided into the major constituents of milk, namely, lipids, proteins, sugars and the mineral fraction.

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Human milk and infant formulae: Peptide differences and the opportunity to address the functional gap

Cited by 7 publications

References 41 publications

Analysis of the Endogenous Peptidomes of Different Infant Formula Types and Human Milk

Analysis of the Endogenous Peptidomes of Different Infant Formula Types and Human Milk

A two years study of dioxin-like polychlorinated biphenyls (dl-PCBs) in mother's milk in Qalyubia governorate, Egypt.

Analysis of milk with liquid chromatography–mass spectrometry: a review

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