HUman MicroNucleus project: international database comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes: I. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei

Bonassi, Stefano; Fenech, Michael; Lando, Cecilia; Lin, Yi-Ping; Ceppi, Marcello; Chang, Wushou P.; Holland, Nina; Kirsch‐Volders, Micheline; Zeiger, Errol; Ban, Sadayuki; Barale, Roberto; Bigatti, Maria Paola; Bolognesi, Claudia; Cao, Jia; Giorgio, Marina Di; Ferguson, Lynnette R.; Fučić, Aleksandra; García, Omar; Hrelia, Patrizia; Krishnaja, A.P.; Lee, Tung‐Kwang; Migliore, Lucia; Mikhalevich, Ludmilla; Mirkova, E; Mosesso, Pasquale; Müller, Wolfgang‐Ulrich; Odagiri, Youichi; Scarffi, Maria Rosaria; Szabová, Elena; Vorobtsova, I.E.; Vral, Anne; Zijno, Andrea

doi:10.1002/1098-2280(2001)37:1<31::aid-em1004>3.0.co;2-p

Cited by 395 publications

(172 citation statements)

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“…Some studies have shown an increase in the frequency of MNC or ONA with age [33,[41][42][43][44] and with the use of mouthwash [45]. According to Barnett and King [46] the influence of age on genotoxic and cytotoxic endpoints possibly reflects the increase in spontaneous chromosome instability with ageing, associated with an accumulation of DNA damage due to a progressive impairment of overall DNA-repair capacity.…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the chosen cell population to monitor for genotoxic damage, Ceppi et al [27] showed that there is a high correlation between the extent of micronucleus formation in buccal exfoliated cells and in cultured peripheral blood lymphocytes. The latter cell type has been extensively used -in a somewhat more invasive methodto evaluate the presence of chromosomal damage in humans [30,37,39,[41][42][43]. In cases of chronic exposure, the differences in kinetics of replication and half-life between buccal cells and lymphocytes, which may affect the occurrence of micronuclei, become irrelevant, since chronic exposure leads to a steady-state elevated expression of micronuclei regardless of division rate, if the period of exposure exceeds the time required for one nuclear division [27].…”

Section: Discussionmentioning

confidence: 99%

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Exposure of thermoelectric power-plant workers to volatile organic compounds from fuel oil: Genotoxic and cytotoxic effects in buccal epithelial cells

Garcia

Linhares

Amaral

et al. 2012

Mutation Research/Genetic Toxicology and Environmental Mutagene

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a b s t r a c tThermoelectric power-plant workers are constantly exposed to high levels of potentially genotoxic gaseous substances, such as volatile organic compounds (VOCs) from the combustion of fuel oil or the processing of naphtha. The aim of the present study was to estimate the association between such occupational exposure and the frequency of micronucleated cells and cells with other nuclear anomalies.Buccal epithelial cells were collected from a total of 44 power-plant workers (exposed group) and 47 administrative workers (non-exposed group), and examined for the frequency of micronucleated cells (MNC) and of cells with other nuclear anomalies (ONA: pyknosis, karyolysis, and karyorrhexis) by means of the micronucleus assay. The frequencies of MNC and ONA per 1000 cells in the exposed group (1.8‰ and 82.4‰, respectively) were significantly higher than in the non-exposed group (0.2‰ and 58.3‰, respectively). The exposed group had a twelve-fold increase in risk for formation of MNC compared with non-exposed individuals (RR = 12.1; 95% CI, 5.0-29.2; P < 0.001). The confounding factors analyzed (age, smoking status, alcohol consumption, and mouthwash use) did not show any significant association with the frequency of MNC or ONA.The findings of this study show that workers from power plants exposed to VOCs have a significantly elevated risk for DNA damage. Therefore, bio-monitoring of DNA damage is recommended for this group of workers.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exposure of thermoelectric power-plant workers to volatile organic compounds from fuel oil: Genotoxic and cytotoxic effects in buccal epithelial cells

Garcia

Linhares

Amaral

et al. 2012

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…It is important in case-control studies that the groups are matched properly for these variables. For more information on the use of controls in biomonitoring studies refer to Albertini et al 76 and for important variables affecting MN frequency in the CBMN assay in human lymphocytes refer to Bonassi et al 77 . The appropriate use of controls in in vitro studies is discussed in detail in the report of the In Vitro Micronucleus Assay Working Group 57 .…”

Section: Controls and Important Variablesmentioning

confidence: 99%

Cytokinesis-block micronucleus cytome assay

2007

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“…In epidemiological studies, it is important to evaluate the role played by common confounding factors, such as gender, age, smoking and alcohol consumption, upon the association between disease and exposure (Bonassi et al, 2001;Fenech at al., 1999). Concerning gender, studies realized by Fenech et al (1999) and Wojda et al (2007) reported that biomarker frequencies were greater in females than in males by a factor of 1.2 to 1.6 depending on the age group.…”

Section: Discussionmentioning

confidence: 99%