Human Microbiome <i>Fusobacterium Nucleatum</i> in Esophageal Cancer Tissue Is Associated with Prognosis

Yamamura, Kensuke; Baba, Yoshifumi; Nakagawa, Shigeki; Mima, Kosuke; Miyake, Keisuke; Nakamura, Kenichi; Shinmoto, Hiroshi; Kinoshita, Koichi; Ishimoto, Takatsugu; Iwatsuki, Masaaki; Sakamoto, Yasuo; Yamashita, Yo‐ichi; Yoshida, Naoya; Watanabe, Masayuki

doi:10.1158/1078-0432.ccr-16-1786

Cited by 315 publications

(240 citation statements)

References 51 publications

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“…Interestingly and in agreement with our predictive results that fusobacteria can home-in and accumulate in high Gal-GalNAc displaying cancers, fusobacterial DNA were reported to be overabundant in pancreas (Mitsuhashi et al, 2015), breast (Hieken et al, 2016), and esophagus (Yamamura et al, 2016) adenocarcinomas and in normal and cancer stomach samples (Dicksved et al, 2009; Nardone and Compare, 2015). This, in addition to the well-known prevalence of fusobacteria in the high Gal-GalNAc -levels displaying colon cancer (Castellarin et al, 2012; Kostic et al, 2012; Abed et al, 2016).…”

Section: Resultssupporting

confidence: 87%

Tumor Targeting by Fusobacterium nucleatum: A Pilot Study and Future Perspectives

Abed

Maalouf

Parhi

et al. 2017

Front. Cell. Infect. Microbiol.

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Colorectal adenocarcinoma (CRC) is a common tumor with high mortality rates. Interestingly, CRC was found to be colonized by the oral anaerobic bacteria Fusobacterium nucleatum, which accelerates tumor progression and enables immune evasion. The CRC-specific colonization by fusobacteria is mediated through the recognition of tumor displayed Gal-GalNAc moieties by the fusobacterial Fap2 Gal-GalNAc lectin. Here, we show high Gal-GalNAc levels in additional adenocarcinomas including those found in the stomach, prostate, ovary, colon, uterus, pancreas, breast, lung, and esophagus. This observation coincides with recent reports that found fusobacterial DNA in some of these tumors. Given the tumorigenic role of fusobacteria and its immune evasion properties, we suggest that fusobacterial elimination might improve treatment outcome of the above tumors. Furthermore, as fusobacteria appears to specifically home-in to Gal-GalNAc—displaying tumors, it might be engineered as a platform for treating CRC and the above common, lethal, adenocarcinomas.

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Section: Resultssupporting

confidence: 87%

Tumor Targeting by Fusobacterium nucleatum: A Pilot Study and Future Perspectives

Abed

Maalouf

Parhi

et al. 2017

Front. Cell. Infect. Microbiol.

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“…Another study, on the other hand, evidenced Fusobacterium nucleatum to possible mediate tumour profileration by activating several chemokines 54 . Microbial shifts may be provoked by altered microenvironments that disturb the normal salivary microbiome and, consequently, lead to a different microbial composition.…”

Section: Discussionmentioning

confidence: 98%

The salivary microbiome as an indicator of carcinogenesis in patients with oropharyngeal squamous cell carcinoma: A pilot study

Wolf

Moissl-Eichinger

Perras

et al. 2017

Sci Rep

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This study aimed to undertake an initial, comparative analysis of the oral salivary microbiome of patients with oral and oropharyngeal squamous cell carcinoma versus healthy controls. This project, conceived as a pilot study, included 11 patients (1 female, 10 male, mean age 61.6 yrs., SD = 8.2 yrs.) and 11 healthy controls (1 female, 10 male, mean age 46.7 yrs., SD = 15.1 yrs.). Samples of saliva were analysed by high-throughput sequencing of the 16S rRNA gene using the MiSeq platform. Sequence data revealed microbial changes that may mirror disease progression and reflect clinical preconditions such as age, alcohol consumption, tumour size, lymph node status, smoking habit, and tumour HPV-positivity. Consequently, mapping microbial changes in patients with oral and oropharyngeal squamous cell carcinomas might improve our understanding of the pathobiology of the disease, and help in the design of novel diagnostic and treatment strategies.

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“…Fusobacteria was not found in the controls with normal esophagus, but was found in 10% of patients with RE and in 9% of those with Barrett's esophageal mucosa . Moreover, it has been suggested that Fusobacterium nucleatum in esophageal adenocarcinoma tissues is associated with shorter survival through the activation of chemokines and by causing aggressive tumor behavior …”

Section: Discussionmentioning

confidence: 96%

Changes in the distal esophageal microbiota in Chinese patients with reflux esophagitis

Gao

Chen

et al. 2018

J of Digest Diseases

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Objective Changes in microbiota composition in the distal esophagus may be associated with the pathogenesis of gastroesophageal reflux disease. We aimed to compare the composition of distal esophageal microbiota between Chinese patients with reflux esophagitis (RE) and healthy volunteers using metagenomic high‐throughput DNA sequencing and bioinformatic analyses. Methods Healthy volunteers (controls) and patients with reflux esophagitis (RE) were enrolled. Distal esophageal (2 cm above the gastroesophageal junction) biopsy specimens were obtained under endoscopy. Microbial DNA was extracted from the specimens, followed by 16S rDNA gene amplification and Illumina sequencing. Bioinformatic tools were applied to dissect the community structure. Results No dramatic differences in microbiota were found in RE patients compared with the controls. At the phylum level, only Bacteroidetes differed between the groups, being less abundant in the RE group. The overall number and diversity of species tended to be lower in RE patients, but there were no significant differences between the groups. Three genera, Prevotella, Helicobacter, and Moraxella, were obviously depleted in RE patients, as revealed by linear discriminant analysis. Conclusions The composition of distal esophageal microbiota in Chinese patients with RE showed moderate changes compared with healthy controls. To what extent these changes are associated with the pathogenesis of RE needs further investigation.

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Human Microbiome Fusobacterium Nucleatum in Esophageal Cancer Tissue Is Associated with Prognosis

Cited by 315 publications

References 51 publications

Tumor Targeting by Fusobacterium nucleatum: A Pilot Study and Future Perspectives

Tumor Targeting by Fusobacterium nucleatum: A Pilot Study and Future Perspectives

The salivary microbiome as an indicator of carcinogenesis in patients with oropharyngeal squamous cell carcinoma: A pilot study

Changes in the distal esophageal microbiota in Chinese patients with reflux esophagitis

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