Human Metapneumovirus and Community-Acquired Respiratory Illness in Children

Vicente, Diego; Cilla, Gustavo; Montes, Milagrosa; Pérez-Trallero, Emilio

doi:10.3201/eid0905.020615

Cited by 82 publications

(97 citation statements)

References 8 publications

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“…However, another recently published study seems to indicate that the association between hMPV and RSV may act in concert, potentiating their pathogenic effects and exacerbating the clinical symptoms of the respiratory disease (Bouscambert-Duchamp et al 2005). Vicente et al (2003) did not find hMPV coinfections with other respiratory viruses in a paediatric population with ARTI, a finding that is discordant from the majority of recently published studies (Vicente et al 2003). In the present report, hMPV was detected as a unique respiratory pathogen in only 2.2% of the cases.…”

Section: Discussioncontrasting

confidence: 56%

Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil

Pilger¹,

Cantarelli

Amantéa

et al. 2011

Mem. Inst. Oswaldo Cruz

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The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents

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Section: Discussioncontrasting

confidence: 56%

Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil

Pilger¹,

Cantarelli

Amantéa

et al. 2011

Mem. Inst. Oswaldo Cruz

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“…hMPV has been identified worldwide [5][6][7][8][9] and appears to have a seasonal distribution (winter and spring) [10]. Since its initial description, hMPV has been identified as a leading cause of LRTIs in previously healthy infant and child outpatients [11].…”

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confidence: 99%

The Role of Human Metapneumovirus in Pediatric Respiratory Tract Infection in Qatar

Althani¹,

Azzam

Abboubaker

et al. 2010

Future Virol.

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Aim: The human metapneumovirus (hMPV) has been recently discovered as an etiological agent of acute respiratory infections in infants and children, with similar clinical symptoms to those caused by respiratory syncytial virus. The aim of this study was to determine the prevalence of hMPV and its potential role as a causative agent of respiratory tract infections in children in Qatar. Methods: In the present study, we examined 84 nasopharyngeal aspirates from children with respiratory tract infections, presenting at Al-Saad Pediatric Emergency Center in Doha, Qatar, as outpatients, for the presence of respiratory viruses. Results: A total of 56 out of 84 (66.7%) cases were positive for the presence of respiratory viruses. Out of the 56 positive cases 54 (96%) contained hMPV; whereas 12 out of 56 (21.4%) contained human parainfluenza virus. A total of 14 out of 56 of the positive patients were infected with more than one virus. hMPV was in samples infected with one or more respiratory tract infection viruses and was the most frequently isolated virus from infants less than 6 months of age. Conclusion: This is the first report demonstrating the prevalence of hMPV in children suffering from respiratory tract infections in Qatar. Detection of this virus may have significant clinical implications in this patient population in Qatar.

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“…HMPV was first identified in respiratory specimens from young children hospitalized with mild to severe lower respiratory tract illness (46), and recent studies indicate that HMPV may cause upper and lower respiratory tract illness in patients between the ages of 2 months and 87 years (5,6,8,23,49,50). The disease burden associated with HMPV infection is not well known and may be complicated by the ability of HMPV to cocirculate with respiratory syncytial virus (RSV) in the community (28,37,50). A recent prospective study of young and older adults hospitalized for respiratory infections during the RSV season showed that HMPV was associated with approx-…”

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confidence: 99%

Human Metapneumovirus Persists in BALB/c Mice despite the Presence of Neutralizing Antibodies

Alvarez¹,

Harrod

Shieh

et al. 2004

J Virol

104

152

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Human metapneumovirus (HMPV) has emerged as an important human respiratory pathogen causing upper and lower respiratory tract infections in young children and older adults. Recent epidemiological evidence indicates that HMPV may cocirculate with respiratory syncytial virus, and HMPV infection has been associated with other respiratory diseases. In this study, we show that BALB/c mice are susceptible to HMPV infection, the virus replicates in the lungs with biphasic growth kinetics in which peak titers occur at days 7 and 14 postinfection (p.i.), and infectious HMPV can be recovered from lungs up to day 60 p.i. In addition, we show that genomic HMPV RNA can be detected in the lungs for >180 days p.i. by reverse transcription-PCR; however, neither HMPV RNA nor infectious virus can be detected in serum, spleen, kidneys, heart, trachea, and brain tissue. Lung histopathology revealed prevalent mononuclear cell infiltration in the interstitium beginning at day 2 p.i. and peaking at day 4 p.i. which decreased by day 14 p.i. and was associated with airway remodeling. Increased mucus production evident at day 2 p.i. was concordant with increased bronchial and bronchiolar inflammation. HMPV-specific antibodies were detected by day 14 p.i., neutralizing antibody titers reached >6.46 log 2 end-point titers by day 28 p. i., and depletion of T cells or NK cells resulted in increased HMPV titers in the lungs, suggesting some immune control of viral persistence. This study shows that BALB/c mice are amenable for HMPV studies and indicates that HMPV persists as infectious virus in the lungs of normal mice for several weeks postinfection.Human metapneumovirus (HMPV) is tentatively a member of the Metapneumovirus genus based on genetic sequence similarity to the type species avian metapneumovirus (46). HMPV was first identified in respiratory specimens from young children hospitalized with mild to severe lower respiratory tract illness (46), and recent studies indicate that HMPV may cause upper and lower respiratory tract illness in patients between the ages of 2 months and 87 years (5,6,8,23,49,50). The disease burden associated with HMPV infection is not well known and may be complicated by the ability of HMPV to cocirculate with respiratory syncytial virus (RSV) in the community (28,37,50). A recent prospective study of young and older adults hospitalized for respiratory infections during the RSV season showed that HMPV was associated with approx-

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Human Metapneumovirus and Community-Acquired Respiratory Illness in Children

Cited by 82 publications

References 8 publications

Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil

Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil

The Role of Human Metapneumovirus in Pediatric Respiratory Tract Infection in Qatar

Human Metapneumovirus Persists in BALB/c Mice despite the Presence of Neutralizing Antibodies

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