Human mesenchymal stem cells successfully improve skin-substitute wound healing

Nakagawa, Hiroshi; Akita, Sadanori; Fukui, Masashi; Fujii, Tohru; Akino, Kozo

doi:10.1111/j.1365-2133.2005.06554.x

Cited by 204 publications

(168 citation statements)

References 22 publications

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“…Thus, the secretion of IL-6 by the constitutive activation of the p38 MAPK pathway in MSCs under wound healing; however, discrepancies existed in exploring the underlying mechanisms. MSCs were found serum depletion or hypoxic conditions may act as one of the endogenous responses to various stresses, endowing to mediate the effects by differentiating into skin (25), vessel (12), or neural cells (16), by secreting paracrine tissues or organs with the ability to resist stresses. The exploration of the roles of MSCs in overcoming the difactors (17) or by combined differentiation and paracrine effects (37).…”

Section: Constitutive Activation Of P38 Mapk Mediatesmentioning

confidence: 99%

“…The therapeutic effects were partly attributed to the multilineage potential of MSCs (12,16, the crucial factors contributing to nonhealing wounds (11). Bone marrow-derived stromal or mesenchymal 25,37). We have previously demonstrated that angiogenic factors secreted by MSCs promoted angiogenesis stem cells (MSCs) are capable of self-renewal and have by activating the PI3K-AKT pathway in endothelial of Adult Stem Cells prepared as described previously (35).…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of Wound Healing by Human Multipotent Stromal Cell Conditioned Medium: The Paracrine Factors and p38 MAPK Activation

Yew¹,

Hung

et al. 2011

Cell Transplant

129

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Wound healing can be improved by transplanting mesenchymal stem cells (MSCs). In this study, we have demonstrated the benefits of the conditioned medium derived from human MSCs (CM-MSC) in wound healing using an excisional wound model. CM-MSC accelerated wound closure with increased reepithelialization, cell infiltration, granulation formation, and angiogenesis. Notably, CM-MSC enhanced epithelial and endothelial cell migration, suggesting the contribution of increased cell migration to wound healing enhanced by CM-MSC. Cytokine array, ELISA analysis, and quantitative RT-PCR revealed high levels of IL-6 in CM-MSC. Moreover, IL-6 added to the preconditioned medium enhanced both cell migration and wound healing, and antibodies against IL-6 blocked the increase in cell motility and wound closure by CM-MSC. The IL-6 secretory pathway of MSCs was inhibited by SB203580, an inhibitor of p38 MAPK or siRNA against p38 MAPK, suggesting IL-6 secretion by MSCs is mediated through the activation of p38 MAPK. Inactivation of p38 MAPK also reduced the expression and production of IL-8 and CXCL1 by MSCs, both of which were also demonstrated to enhance cell migration and wound closure. Thus, our data suggest MSCs promote wound healing through releasing a repertoire of paracrine factors via activation of p38 MAPK, and the CM-MSC may be applied to enhance wound healing.

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Section: Constitutive Activation Of P38 Mapk Mediatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancement of Wound Healing by Human Multipotent Stromal Cell Conditioned Medium: The Paracrine Factors and p38 MAPK Activation

Yew¹,

Hung

et al. 2011

Cell Transplant

129

View full text Add to dashboard Cite

show abstract

“…Bone-marrow mesenchymal stem cells (BM-MSC) are capable of selfrenewing and of differentiating into various tissues and cells, such as liver epithelium, lung, gastrointestinal tract, and skin cells [204,205]. In fact it was shown that allogeneic BMMSCs significantly enhanced wound healing in normal and diabetic mice through differentiation and release of proangiogenic factors [206].…”

Section: Cell-based Therapiesmentioning

confidence: 99%

Inflammatory and Angiogenic Abnormalities in Diabetic Wound Healing: Role of Neuropeptides and Therapeutic Perspectives

Tellechea¹

2012

TOCVJ

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Diabetic foot ulceration (DFU) is one of the most costly and debilitating complications of diabetes and is the leading cause of non-traumatic amputations, affecting 15% of the diabetic population. Impaired wound healing in diabetic patients without large-vessel disease has been attributed to microvascular dysfunction, neuropathy, and abnormal cellular and inflammatory responses. These abnormalities have been examined mainly in animal models although a few studies have been undertaken in diabetic patients. This review provides an overview of the inflammatory and vascular abnormalities in DFU and emphasises the role of angiogenic growth factors, endothelial progenitor cells (EPCs), and neuropeptides as mediators of wound healing and potential therapeutic agents for these chronic, non-healing ulcers.

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“…The MSCs have been shown to possess a strong ability to improve tissue damage in response to skin injury, by contributing to collagen deposition (60), wound contraction (61), angiogenesis (62), regeneration of skin appendages, and enhanced growth of epidermal cells (63). Currently, most of the studies related to MSCs in clinical treatments are on BMMSCs; only limited studies mentioned the application of WJMSCs.…”

Section: Wharton's Jelly Multipotent Stem Cells and Skin Reconstructionmentioning

confidence: 99%

The Role of Wharton’s Jelly Mesenchymal Stem Cells in Skin Reconstruction

et al. 2015

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Context: Stem cell therapy, especially in the segment of mesenchymal stem cells (MSCs), is one of the most promising areas of regenerative medicine. Evidence Acquisition: According to research conducted by various researchers, Wharton's Jelly mesenchymal stem cells (WJMSCs) have several advantages compared to others sources, in regenerative medicine: WJMSCs are more primary cells; WJMSCs can be easily isolated and without invasive procedures; WJMSCs have no ethical problems; WJMSCs are more cost effective than other sources of MSCs. Also, WJMSCs were demonstrated to express stem cell mesenchymal markers. Results: Similar to bone marrow MSCs, WJMSCs express major histocompatibility complex (MHC) class I molecules. Conclusions: Although the aforementioned challenges must still be addressed, the potential of WJMSCs in skin regenerative clinical treatments is promising.

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Human mesenchymal stem cells successfully improve skin-substitute wound healing

Abstract: These data suggest that hMSCs together with bFGF in a skin defect model accelerate cutaneous wound healing as the hMSCs transdifferentiate into the epithelium.

Cited by 204 publications

References 22 publications

Enhancement of Wound Healing by Human Multipotent Stromal Cell Conditioned Medium: The Paracrine Factors and p38 MAPK Activation

Enhancement of Wound Healing by Human Multipotent Stromal Cell Conditioned Medium: The Paracrine Factors and p38 MAPK Activation

Inflammatory and Angiogenic Abnormalities in Diabetic Wound Healing: Role of Neuropeptides and Therapeutic Perspectives

The Role of Wharton’s Jelly Mesenchymal Stem Cells in Skin Reconstruction

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