Human Mesenchymal Stem Cells Signals Regulate Neural Stem Cell Fate

Bai, Lianhua; Caplan, Arnold I.; Lennon, Donald P.; Miller, Robert H.

doi:10.1007/s11064-006-9212-x

Cited by 87 publications

(70 citation statements)

References 44 publications

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“…Its role is also consistent with the observation that conditioned medium from hMSCs increased oligodendrogenesis by rNSCs incubated in medium containing 10% FBS [41,42]. hMSCs also increase both oligodendrogenesis and neuronogenesis by mouse neurospheres incubated in serum-free medium [43]. In the optimal NSC growth medium used here, conditioned medium from hMSCs increased differentiation to oligodendrocytes but not astrocytes.…”

Section: Discussionsupporting

confidence: 89%

Human Stem/Progenitor Cells from Bone Marrow Enhance Glial Differentiation of Rat Neural Stem Cells: A Role for Transforming Growth Factor β and Notch Signaling

Robinson

Foraker

Ylöstalo

et al. 2011

Stem Cells and Development

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Multipotent stem=progenitor cells from bone marrow stroma (mesenchymal stromal cells or MSCs) were previously shown to enhance proliferation and differentiation of neural stem cells (NSCs) in vivo, but the molecular basis of the effect was not defined. Here coculturing human MSCs (hMSCs) with rat NSCs (rNSCs) was found to stimulate astrocyte and oligodendrocyte differentiation of the rNSCs. To survey the signaling pathways involved, RNA from the cocultures was analyzed by species-specific microarrays. In the hMSCs, there was an upregulation of transcripts for several secreted factors linked to differentiation: bone morphogenetic protein 1 (BMP1), hepatocyte growth factor (HGF), and transforming growth factor isoforms (TGFb1 and TGFb3). In both the hMSCs and the rNSCs, there was an upregulation of transcripts for Notch signaling. The role of TGFb1 was verified by the demonstration that hMSCs in coculture increased secretion of TGFb1, the rNSCs expressed the receptor, and an inhibitor of TGFb signaling blocked differentiation. The role of Notch signaling was verified by the demonstration that in the cocultures hMSCs expressed a Notch ligand at sites of cell contact with rNSCs, and the rNSCs expressed the receptor, Notch 1. Increased Notch signaling in both cell types was then demonstrated by assays of transcript expression and by a reporter construct for downstream targets of Notch signaling. The results demonstrated that glial differentiation of the rNSCs in the cocultures was driven by increased secretion of soluble factors such as TGFb1 by the hMSCs and probably through increased cell contact signaling between the hMSCs and rNSCs through the Notch pathway.

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Section: Discussionsupporting

confidence: 89%

Human Stem/Progenitor Cells from Bone Marrow Enhance Glial Differentiation of Rat Neural Stem Cells: A Role for Transforming Growth Factor β and Notch Signaling

Robinson

Foraker

Ylöstalo

et al. 2011

Stem Cells and Development

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“…It has been reported that a soluble factor secreted by a stromal cell feeder layer is essential for stimulating OE neuronal cells to form colonies in vitro (Mumm et al, 1996;Shou et al, 2000). Others have shown using human MSCs from the bone marrow, that they are able to regulate the differentiation of neural stem cell fate (Bai et al, 2007;Croft and Przyborski, 2008). In these experiments MSCs promoted the differentiation of neurosphere-derived stem cells from the CNS by the secretion of a soluble factor.…”

Section: Cellular Composition Of the Lpmentioning

confidence: 99%

Olfactory mucosa for transplant‐mediated repair: A complex tissue for a complex injury?

Lindsay

Riddell

Barnett

2009

Glia

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Damage to the brain and spinal cord leads to permanent functional disability because of the very limited capacity of the central nervous system (CNS) for repair. Transplantation of cells into regions of CNS damage represents one approach to enhancing this repair. At present, the ideal cell type for transplant-mediated repair has not been identified but autologous transplantation would be advantageous. Olfactory tissue, in part because of its capacity for regeneration, has emerged as a promising source of cells and several clinical centers are using olfactory cells or tissues in the treatment of CNS damage. Until now, the olfactory ensheathing cell, a specialized glial cell of the olfactory system has been the main focus of attention. Transplants of this cell have been shown to have a neuroprotective function, support axonal regeneration, and remyelinate demyelinated axons. However, the olfactory mucosa is a heterogeneous tissue, composed of a variety of cells supporting both its normal function and its regenerative capacity. It is therefore possible that it contains several cell types that could participate in CNS repair including putative stem cells as well as glia. Here we review the cellular composition of the olfactory tissue and the evidence that equivalent cell types exist in both rodent and human olfactory mucosa suggesting that it is potentially a rich source of autologous cells for transplant-mediated repair of the CNS.

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“…14 They have been used in preclinical models for TE bone, cartilage, muscle, marrow stroma, tendon, fat and other connective tissues. [15][16][17][18][19][20][21] The development of scaffolds possessing characteristics optimal for the induction of MSC-mediated chondrogenesis using a TE approach is of great interest. [22][23][24] Indeed, previous work in our laboratory has shown that by altering the composition and mechanical properties of CG scaffolds, the differentiation of MSCs towards a chondrogenic lineage can be significantly affected.…”

Section: Introductionmentioning

confidence: 99%

Scaffold Mean Pore Size Influences Mesenchymal Stem Cell Chondrogenic Differentiation and Matrix Deposition

Matsiko

Gleeson

O’Brien

2015

Tissue Engineering Part A

220

170

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FJ. Scaffold mean pore size influences mesenchymal stem cell chondrogenic differentiation and matrix deposition. Tissue Engineering. Part A. 2015;21(3-4):486-97. -Use Licence - AbstractRecent investigations into micro-architecture of scaffolds has revealed that mean pore sizes are cell type specific and influence cellular shape, differentiation and extracellular matrix secretion.In this context, the overall goal of this study was to investigate whether scaffold mean pore sizes affect mesenchymal stem cell initial attachment, chondrogenic gene expression and cartilage-like matrix deposition. Collagen-hyaluronic acid (CHyA) scaffolds, recently developed in our laboratory for in vitro chondrogenesis, were fabricated with three distinct mean pore sizes (94 μm, 130 μm and 300 μm) by altering the freeze-drying technique used. It was evident that scaffolds with the largest mean pore sizes (300 μm) stimulated significantly higher cell proliferation, chondrogenic gene expression and cartilage-like matrix deposition relative to scaffolds with smaller mean pore sizes (94 μm, 130 μm). Taken together, these findings demonstrate the importance of scaffold micro-architecture in the development of advanced tissue engineering strategies for articular cartilage defect repair.

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Human Mesenchymal Stem Cells Signals Regulate Neural Stem Cell Fate

Cited by 87 publications

References 44 publications

Human Stem/Progenitor Cells from Bone Marrow Enhance Glial Differentiation of Rat Neural Stem Cells: A Role for Transforming Growth Factor β and Notch Signaling

Human Stem/Progenitor Cells from Bone Marrow Enhance Glial Differentiation of Rat Neural Stem Cells: A Role for Transforming Growth Factor β and Notch Signaling

Olfactory mucosa for transplant‐mediated repair: A complex tissue for a complex injury?

Scaffold Mean Pore Size Influences Mesenchymal Stem Cell Chondrogenic Differentiation and Matrix Deposition

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