Human mesenchymal stem cell derived osteoblasts degrade organic bone matrix in vitro by matrix metalloproteinases

Extracellular Matrix Remodeling: The Common Denominator in Connective Tissue DiseasesPossibilities for Evaluation and Current Understanding of the Matrix as More Than a Passive Architecture, but a Key Player in Tissue Failure

“…49 The synthesis, concentration, and circulating levels (serum concentration) of degradation products of type I collagen have been proven to be increased during breast, bone, lung, ovarian, prostate, and skin malignancy. [50][51][52][53][54][55] Type II collagen is the major component of hyaline cartilage, but is also found in the vitreous body of the eye, the corneal epithelium, the notochord, the nucleus pulposus of invertebral discs, and embryonic epithelial-to-mesenchymal transitions (EMTs). 47 Type II collagen is a homotrimer consisting of three a 1 (II) chains, and the primary sequence has a high content of hydroxylysine and glycosyl residues, which mediate interactions with proteoglycans, another important component of hyaline cartilage.…”

Matrix Metalloprotease Activity Is an Essential Link Between Mechanical Stimulus and Mesenchymal Stem Cell Behavior

“…Alterations to MMP activity, for example, could lead to the degradation of extracellular matrix components like collagen, thereby removing mechanical barriers or exposing cryptic sites that could act to regulate MSCs [45,46]. Furthermore, the modulation of growth factor activity or bioavailability is another likely mechanism of MMP impact on MSC behavior [9].…”

Akt signaling regulates actin organization via modulation of MMP‐2 activity during chondrogenesis of chick wing limb bud mesenchymal cells

“…There are few systematic studies on the expression patterns of the different MMPs and their inhibitors in cartilage and bone [Tamamura et al, 2005;Fichter et al, 2006;Soder et al, 2006]. MMP-9 (92 kDa type IV collagenase/gelatinase B) and MMP-2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase) are known to have an important roles in osteoclastic bone resorption [Parikka et al, 2005;Reinhardt et al, 2005] and cartilage remodeling Milner et al, 2006]. To examine the time points and level of induction of gelatinases during the late period of micromass culture, conditioned media of chondroblasts taken at 3-, 4-and 5-day time points were analyzed by zymography.…”