Human menstrual blood–derived stem cells promote the repair of impaired endometrial stromal cells by activating the p38 MAPK and AKT signaling pathways

Zhu, Haiyan; Jiang, Yinshen; Pan, Y. B.; Shi, Libing

doi:10.1016/j.repbio.2018.06.003

Cited by 31 publications

(18 citation statements)

References 33 publications

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“…A previous study demonstrated increased expression of keratin in endometrial tissues (41). In agreement with the results of the present study, Zhu et al (19) demonstrated that MenSC-based therapies may represent promising methods for the treatment of endometrial injury via the regulation of AKT and p38 signalling pathways.…”

Section: Discussionsupporting

confidence: 93%

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Effects of menstrual blood‑derived stem cells on endometrial injury repair

Song

Zhang

et al. 2018

Mol Med Report

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The present study aimed to investigate the effects of menstrual blood-derived stem cells (MenSCs) on endometrial injury repair. MenSCs were isolated from human menstrual blood and were cultured in vitro. Flow cytometric analysis of cells in the third generation demonstrated that MenSCs exhibited higher expression levels of cluster of differentiation (CD)90 and lower expression levels of CD146, which suggested that the MenSCs were cultured successfully. A mechanical damage model of unilateral (right) endometrium was established in BALB/c nude mice, which were divided into four groups, Normal, negative control (NC), Model and MenSC. MenSCs transfected with adenovirus-enhanced green fluorescent protein were transplanted into the right uterine cavity of mice in the MenSC and NC groups. The protein expression levels of keratin, vimentin, and vascular endothelial growth factor (VEGF) and the average endometrial thickness were measured by immunohistochemistry; the average optical density of vimentin, VEGF and keratin in the MenSC-treated group was significantly higher compared with the untreated Model group. Fertility tests were performed to determine the pregnancy rate of each group; following endometrial damage in BALB/c nude mice, endometrial thickness was decreased in the Model group, whereas model mice treated with MenSC exhibited increased endometrial thickness and increased the pregnancy rates. Therefore, MenSCs may promote the repair of endometrial lesions in mice by promoting the expression of vimentin, VEGF and keratin.

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Section: Discussionsupporting

confidence: 93%

“…At the same time, because menstrual blood is considered human waste, investigators were able to avoid the problem of sensitive medical ethical issues. The use of menstrual blood-derived stem cell (MenSC) transplantation for repair of the endometrial injury in nude mice with endometrial injury has been previously investigated (19).…”

Section: Introductionmentioning

confidence: 99%

Effects of menstrual blood‑derived stem cells on endometrial injury repair

Song

Zhang

et al. 2018

Mol Med Report

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“…Alcayaga-Miranda et al discovered that MenSCs possessed a superior capacity of the migration when compared with BM-MSCs, and the migratory properties were predominantly mediated by some integrins, selectins, and chemokine receptors [51]. Furthermore, Zhu et al proved that MenSCs notably increased the proliferation and migration capacity of impaired endometrial stromal cells in vitro, which provided a vital foundation for treating endometrial-related injury [60]. Interestingly, Wang et al demonstrated that transplantation of MenSCs effectively ameliorated cisplatin-induced POF in mice, and the preferential contributor was directive migration into ovarian interstices to regulate the microenvironment of the organism [61].…”

Section: Therapeutic Mechanisms Of Menscsmentioning

confidence: 99%

“…Therefore, the manner in which MenSCs are injected should be uniformed and agreed upon for further studies of the same disease. It has been demonstrated that MenSC transplantation was safe when evaluating transplanted cells in both animal models and clinical trials [38, 60, 74, 90, 102]. According to Table 1, the collection time post MenSC administration is different (from 2 to 40 days) in pre-clinical research in vivo.…”

Section: Future Perspectives Of Menscs In Treating Diseasesmentioning

confidence: 99%

Menstrual blood-derived stem cells: toward therapeutic mechanisms, novel strategies, and future perspectives in the treatment of diseases

Chen

Cheng

et al. 2019

Stem Cell Res Ther

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Menstrual blood-derived stem cells (MenSCs) have great potential in the treatment of various diseases. As a novel type of mesenchymal stem cells (MSCs), MenSCs have attracted more interest due to their therapeutic effects in both animal models and clinical trials. Here, we described the differentiation, immunomodulation, paracrine, homing, and engraftment mechanisms of MenSCs. These include differentiation into targeting cells, immunomodulation with various immune cells, the paracrine effect on secreting cytokines, and homing and engraftment into injured sites. To better conduct MenSC-based therapy, some novel hotspots were proposed such as CRISPR (clustered regularly interspaced short palindromic repeats)/cas9-mediated gene modification, exosomes for cell-free therapy, single-cell RNA sequence for precision medicine, engineered MenSC-based therapy for the delivery platform, and stem cell niches for improving microenvironment. Subsequently, current challenges were elaborated on, with regard to age of donor, dose of MenSCs, transplantation route, and monitoring time. The management of clinical research with respect to MenSC-based therapy in diseases will become more normative and strict. Thus, a more comprehensive horizon should be considered that includes a combination of traditional solutions and novel strategies. In summary, MenSC-based treatment has a great potential in treating diseases through diverse strategies, and more therapeutic mechanisms and novel strategies need to be elucidated for future regenerative medicine and clinical applications.

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“…It is suggested that eEPs are located in the base of the glands and are the source of the proliferative cells for re-epithelialization [ 205 ]. A recent study has proposed that endometrial stem cells can promote the repair of stromal cells by activating the p38 MAPK and Akt signaling pathways [ 206 ]. Deep sequencing and epigenetic profiling of endometrial stem/progenitor cells and their differentiated progeny will shed new light on their regulations and functions.…”

Section: Regeneration: Repairing the Functionalismentioning

confidence: 99%

Inside the Endometrial Cell Signaling Subway: Mind the Gap(s)

Makieva

Giacomini

Ottolina

et al. 2018

IJMS

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Endometrial cells perceive and respond to their microenvironment forming the basis of endometrial homeostasis. Errors in endometrial cell signaling are responsible for a wide spectrum of endometrial pathologies ranging from infertility to cancer. Intensive research over the years has been decoding the sophisticated molecular means by which endometrial cells communicate to each other and with the embryo. The objective of this review is to provide the scientific community with the first overview of key endometrial cell signaling pathways operating throughout the menstrual cycle. On this basis, a comprehensive and critical assessment of the literature was performed to provide the tools for the authorship of this narrative review summarizing the pivotal components and signaling cascades operating during seven endometrial cell fate “routes”: proliferation, decidualization, implantation, migration, breakdown, regeneration, and angiogenesis. Albeit schematically presented as separate transit routes in a subway network and narrated in a distinct fashion, the majority of the time these routes overlap or occur simultaneously within endometrial cells. This review facilitates identification of novel trajectories of research in endometrial cellular communication and signaling. The meticulous study of endometrial signaling pathways potentiates both the discovery of novel therapeutic targets to tackle disease and vanguard fertility approaches.

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Human menstrual blood–derived stem cells promote the repair of impaired endometrial stromal cells by activating the p38 MAPK and AKT signaling pathways

Cited by 31 publications

References 33 publications

Effects of menstrual blood‑derived stem cells on endometrial injury repair

Effects of menstrual blood‑derived stem cells on endometrial injury repair

Menstrual blood-derived stem cells: toward therapeutic mechanisms, novel strategies, and future perspectives in the treatment of diseases

Inside the Endometrial Cell Signaling Subway: Mind the Gap(s)

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