Human Melanocytes in Tissue Culture1

Hu, Funan; Staricco, Renato J.; Pinkus, Hermann; Fosnaugh, Robert P.

doi:10.1038/jid.1957.3

Cited by 57 publications

(17 citation statements)

References 14 publications

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“…The first account of human melanocytes cultured from skin was published by Hu et al., in the Journal of Investigative Dermatology in 1957 . The authors used explants of skin, embedded in a ‘plasma‐embryonic extract clot’ with ‘human ascetic fluid’ in roller tubes.…”

Section: Background: Roller Bottles and ‘Plasma‐embryonic Extract’mentioning

confidence: 99%

Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin by Eisinger and Marko

Scott

2013

Experimental Dermatology

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Melanocytes are pigment producing cells that arise from the neural crest and migrate to the skin early in fetal development. The pigment that melanocytes synthesize, melanin, plays a critical role in protecting the skin from mutagenic ultraviolet irradiation. Melanocytes are also precursor cells for melanoma, a deadly form of skin cancer. Because melanocytes make up a minority population of cells in the epidermis they have been difficult to propagate in culture. The landmark paper by Eisinger and Marko, described below, was the first successful report of large scale propagation of pure cultures of melanocytes. This paper set the stage for an explosive growth in knowledge in the biology of human melanocytes and allowed scientists to begin dissecting the different oncogenic events involved in the transition of melanocytes to melanoma.

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Section: Background: Roller Bottles and ‘Plasma‐embryonic Extract’mentioning

confidence: 99%

Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin by Eisinger and Marko

Scott

2013

Experimental Dermatology

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“…This means shifting away from the mitogens phorbol myristate acetate/12-tetradecanoylphorbol 13-acetate (PMA/TPA) [1,2] and melanogenesis enhancers isobutylmethylxanthine (IBMX) or cholera toxin (CTX), incompatible with therapeutic applications due to their tumorigenic effect or strong interference with cellular signalling. [1][2][3][4][5][12][13][14][15][16][17] Essential ingredients for in vitro proliferation without such side effects, fetal bovine serum (FBS) or bovine pituitary extract (BPE) have effects analogous to human serum (HS) [18][19][20] but are unsuitable for therapeutic purposes due to potential prion transmission.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9][10][11] However, the emerging demand for cell therapies for skin regeneration and repigmentation requires conformity with medicinal product-grade melanocytes.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5][12][13][14][15][16][17] Essential ingredients for in vitro proliferation without such side effects, fetal bovine serum (FBS) or bovine pituitary extract (BPE) have effects analogous to human serum (HS) [18][19][20] but are unsuitable for therapeutic purposes due to potential prion transmission. [21,22] As a step in the translation of human melanocytes cultivated from the outer root sheath of hair follicle (HUMORS), we developed a me- …”

Section: Introductionmentioning

confidence: 99%

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A human serum‐enriched medium formulation supports high viability and marker expression in primary melanocyte cultures from the outer root sheath and epidermis

Schneider

Lohrenz

Cross

et al. 2017

Experimental Dermatology

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Formulating clinically relevant melanocyte cultivation media that maintain the balance between proliferation and maturation to functional melanocytes is a major experimental and regulatory challenge. Having established an application-directed BPE-free formulation, we then re-formulated a research-grade medium with BPE for purposes of even more effective in vitro cultivation, adjusted to specific requirements of HUMORS and normal human epidermal melanocytes (NHEM).

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“…Several methods and several media formulations have been published over the years for the separation and subsequent culturing of human epidermal melanocytes and keratinocytes (Eisinger et al, 1982;Gilchrest et al, 1984;Hu et al, 1957). With few exceptions, all methods used chemical mitogens to enhance cell growth.…”

Section: Local Wound Care Studiesmentioning

confidence: 99%

Advanced wound care: from genetics to skin cell therapy

Szabad¹

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Human Melanocytes in Tissue Culture1

Cited by 57 publications

References 14 publications

Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin by Eisinger and Marko

Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin by Eisinger and Marko

A human serum‐enriched medium formulation supports high viability and marker expression in primary melanocyte cultures from the outer root sheath and epidermis

Advanced wound care: from genetics to skin cell therapy

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