Human macrophages stimulate expression of inflammatory mediators in adipocytes; effects of second-generation antipsychotics and glucocorticoids on cellular cross-talk

Modulation of hypothalamic AMPK phosphorylation by olanzapine controls energy balance and body weight

“…administration. These results are reinforced by our previous ex vivo studies in human WAT explants describing the inability of SGAs to alter the expression of adipokines or genes involved in energy regulation [42,88]. Moreover, the hypothalamic-iWAT connection by OLA leading to browning paralleled with a reduction of hypothalamic phospho-AMPK in agreement with the work of Martins et al on the effect of BMP8b on energy balance [49].…”

Effects of the second-generation antipsychotic drugs aripiprazole and olanzapine on human adipocyte differentiation

“…The differentiation continued using maintenance media (composition is the same as the induction cocktail, but without IBMX) until day 14. During the complete course of differentiation (14 days), cells were incubated in the absence or presence of either vehicle control (dimethyl sulfoxide, DMSO, Sigma) or therapeutic and supra-therapeutic concentrations of SGAs: ARI 1, 2, and 10 μM (AC457990010; ACROS Organics, ThermoFisher); ARI's active metabolite dehydroaripiprazole (DARI) 0.4 and 4 μM (1042690; US Pharmacopeial Convention, Rockville, USA); and OLA 0.2 and 2 μM, (GP8311, Glentham Life Sciences, Corsham, UK) as shown previously in the treatment of human adipose tissue ex vivo (Sarsenbayeva et al, 2019(Sarsenbayeva et al, , 2021. The drugs were present in the induction and maintenance differentiation medium.…”

“…Our recent study showed that ARI and OLA at therapeutic concentrations have a mild direct effect on lipid and glucose metabolism of mature adipocytes after short-term adipose tissue exposure (up to 72h) (Sarsenbayeva et al, 2019;Sarsenbayeva et al, 2021). This data suggests that SGAs might affect adipocytes before they are mature (during differentiation), longer/chronic treatments might be needed, or the adverse effects could be mediated through other insulin-sensitive tissues and/or the central nervous system.…”

Identification of potential molecular mechanisms and therapeutic targets for recurrent pelvic organ prolapse

“…Evidence on the relationship between other genes and POP is limited. These five remaining hub genes with research prospects deserve further exploration, especially the regulatory effects of ADIPOQ on hormone progress [ 50 ], PPARG on adipocyte differentiation [ 51 ], CEBPA on body weight homeostasis [ 52 ], and LPL and LIPE on triglyceride metabolism need to be determined [ 53 ].…”