Human lung tumours may coexpress different classes of intermediate filaments.

Gatter, K C; Dunnill, M. S.; Muijen, G N van; Mason, D. Y.

doi:10.1136/jcp.39.9.950

Cited by 84 publications

(23 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, vimentin expression is increased in lung cancers, as well as many other cancers of epithelial origin (20,38). The significance of the activation of vimentin expression is twofold.…”

mentioning

confidence: 99%

Poly(ADP-ribose) polymerase-1 regulates vimentin expression in lung cancer cells

Chu

Xu²,

Ferro³

et al. 2007

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

Vimentin is one of the mammalian intermediate filament proteins. It is expressed in cells of mesenchymal origin and is characteristic of proliferating cells at the fetal stage. During malignancy, vimentin expression is activated in certain lung epithelial cells. Examination of a group of lung cancer cells showed a marked difference in their vimentin expression. The difference in vimentin expression among lung cancer cells is due to differential regulation at the transcriptional level. Analysis of the vimentin promoter revealed a 102-bp promoter sequence that is important for promoter activity in a lung cancer cell line in which vimentin is strongly expressed. This promoter region interacts with poly(ADP-ribose) polymerase-1 (PARP-1), which is also a transcription regulator. Exogenous expression of PARP-1 increased vimentin promoter activity. A shortened PARP-1 without the COOH-terminal catalytic domain showed the same promoter activation effect. Treatment of cells with H2O2 reduced PARP-1 and vimentin expression at the protein level. H2O2 also dose dependently suppressed vimentin promoter activity in cells overexpressing PARP-1. These results demonstrate that vimentin expression in lung cancer cells is regulated at the transcriptional level and that PARP-1 binds and activates the vimentin promoter independent of its catalytic domain and may play a role in H2O2-induced inhibition of vimentin expression.

show abstract

mentioning

confidence: 99%

Poly(ADP-ribose) polymerase-1 regulates vimentin expression in lung cancer cells

Chu

Xu²,

Ferro³

et al. 2007

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

show abstract

“…This is consistent with the level present in primary samples of poorly differentiated squamous carcinomas where only scattered positive cells are found while large areas of well-differentiated tumours are reported positive (Broers et al, 1988). The co-expression of cytokeratins and vimentin in primary lung squamous carcinomas has also been reported (Gatter et al, 1986) and these cell lines demonstrate both types of intermediate filament.…”

Section: Discussionmentioning

confidence: 99%

12-O-Tetradecanoylphorbol 13-acetate induced differentiation in human lung squamous carcinoma cells

1993

Lung Cancer

View full text Add to dashboard Cite

Summary Three human lung squamous carcinoma cell lines (NX002, CX140 and CX143) demonstrate features of squamous differentiation including involucrin synthesis and competence to form comified envelopes. 12-O-Tetradecanoylphorbol 13-acetate inhibits growth of these cell lines and this growth inhibition is associated with enhanced differentiation.Squamous cell carcinoma is the most common subtype of non-small cell lung cancer and represents 40% of all lung cancer cases (Minna et al., 1989). The factors that control proliferation and differentiation in this disease are poorly understood and an improved knowledge of these might aid the development of therapeutic strategies such as the use of differentiation induction in this tumour type (Bloch, 1984;Sartorelli, 1985). Lung squamous carcinoma cells undergoing terminal differentiation demonstrate a number of features including expression of involucrin (a protein precursor found beneath the plasma membrane prior to terminal differentiation) and the ability to form cornified envelopes (Miyazaki et al., 1982;Banks-Schlegel et al., 1985;Levitt et al., 1990;Said et al., 1983;Salge et al., 1990 (Broers et al., 1988).In the present study, we describe differentiation features within three new cell lines and show how they relate to the xenografts from which they were derived. The effect of the differentiation inducer 12-O-tetradecanoylphorbol 13-acetate (TPA) on the growth and differentiation of these cell lines was then investigated. Materials and methodsThe three xenografts from which the cell lines were derived were obtained from untreated patients in 1983 and brief details of these xenografts have previously been reported (Fergusson et al., 1986). The pathology of both the NX002 and CX140 xenografts is consistent with that of poorly differentiated lung squamous carcinoma and has remained constant over the last 7 years. The CX143 xenograft was obtained from a patient with adenosquamous carcinoma and although both adeno and squamous components could be observed in early passages of the xenograft, the pathology of later passages was consistent with a poorly differentiated squamous carcinoma.Cell lines were derived from all three xenografts between their 20th and 27th passages and grown in RPMI 1640 media supplemented with 5% foetal calf serum (FCS), streptomycin (100 igml-'), penicillin (1OOIUml-') and glutamine (2g.M) and kept at 37°C in 5% CO2 and 90% humidity. For these experiments, cell lines were in their 6th-20th passage.For experiments wherein the effects of TPA on growth were examined, cells in exponential phase of growth were trypsinised and plated into 24 well trays at a density of 5 x I04 cells/well. Twenty-four hours later, TPA at concentrations ranging from 10`0 to 10-6 M was added to quadruplicate wells. After 4 days, cells were trypsinised and counted using a ZF coulter counter.For detection of antigens, cells were stained by an indirect immunoperoxidase method using avidin-biotinylated horseradish peroxidase complex (Hsu et al., 1981). Mouse monoclonal anti...

show abstract

“…Many neuroendocrine carcinomas express more than one class of intermediate filaments, often coexpress cytokeratin and NFs (Gatter et al, 1986;Lehto et al, 1985;Merot et al, 1986;Miettinen et al, 1985;Ramaekers et al, 1987) and this coexpression may be related to a special histogenesis. Tumours with an established neural crest derivation display divergence in their intermediate filament expression, probably reflecting the early segregation of neural crest cells before migration and their different susceptibility to environmental influences (Ziller et al, 1983).…”

Section: Resultsmentioning

confidence: 99%

“…The immunohistochemical demonstration of IF proteins in tumour cells is widely recognised as an index of cellular differentiation and this approach is therefore extensively used in the histologic diagnosis of human tumours (Battifora, 1988;Parentes & Rubenstein, 1987;Puts et al, 1986). Coexpression of at least two different classes of IFs may be seen in some types of malignancy, notably among lung tumours (Blobel et al, 1985;Gatter et al, 1986;Lehto et al, 1985;Ramaekers et al, 1987).…”

mentioning

confidence: 99%

Differential expression of neurofilament triplet proteins in carcinoid tumours: an immunohistochemical study

Astarloa

Sánchez‐Franco²,

Cacicedo³

et al. 1991

Br J Cancer

View full text Add to dashboard Cite

Human lung tumours may coexpress different classes of intermediate filaments.

Cited by 84 publications

References 25 publications

Poly(ADP-ribose) polymerase-1 regulates vimentin expression in lung cancer cells

Poly(ADP-ribose) polymerase-1 regulates vimentin expression in lung cancer cells

12-O-Tetradecanoylphorbol 13-acetate induced differentiation in human lung squamous carcinoma cells

Differential expression of neurofilament triplet proteins in carcinoid tumours: an immunohistochemical study

Contact Info

Product

Resources

About