Human liver autofluorescence: An intrinsic tissue parameter discriminating normal and diseased conditions

Croce, Anna Cleta; Simone, Uliana De; Freitas, Isabel; Boncompagni, Eleonora; Neri, Daniele; Cillo, Umberto; Bottiroli, Giovanni

doi:10.1002/lsm.20923

Cited by 45 publications

(48 citation statements)

References 39 publications

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“…The procedures followed for the fitting analysis were already described in detail, as to both the choice of half-Gaussian Modified Gaussian (GMG) spectral function parameters characterizing each single fluorophore and the estimation of their contribution to the AF overall signal [22, 27, 33]. In brief, the spectral functions describing the emission profile of each single fluorophore involved in liver tissue AF were combined by means of subsequent adjustments to match the best fit of the curve representing their sum with the experimental spectrum profile.…”

Section: Methodsmentioning

confidence: 99%

Integrated Autofluorescence Characterization of a Modified-Diet Liver Model with Accumulation of Lipids and Oxidative Stress

Croce

Ferrigno

Piccolini

et al. 2014

BioMed Research International

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Oxidative stress in fatty livers is mainly generated by impaired mitochondrial β-oxidation, inducing tissue damages and disease progression. Under suitable excitation, light liver endogenous fluorophores can give rise to autofluorescence (AF) emission, the properties of which depend on the organ morphofunctional state. In this work, we characterized the AF properties of a rat liver model of lipid accumulation and oxidative stress, induced by a 1–9-week hypercaloric methionine-choline deficient (MCD) diet administration. The AF analysis (excitation at 366 nm) was performed in vivo, via fiber optic probe, or ex vivo. The contribution of endogenous fluorophores involved in redox reactions and in tissue organization was estimated through spectral curve fitting analysis, and AF results were validated by means of different histochemical and biochemical assays (lipids, collagen, vitamin A, ROS, peroxidised proteins, and lipid peroxidation -TBARS-, GSH, and ATP). In comparison with the control, AF spectra changes found already at 1 week of MCD diet reflect alterations both in tissue composition and organization (proteins, lipopigments, and vitamin A) and in oxidoreductive pathway engagement (NAD(P)H, flavins), with a subsequent attempt to recover redox homeostasis. These data confirm the AF analysis potential to provide a comprehensive diagnostic information on negative effects of oxidative metabolism alteration.

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Section: Methodsmentioning

confidence: 99%

Integrated Autofluorescence Characterization of a Modified-Diet Liver Model with Accumulation of Lipids and Oxidative Stress

Croce

Ferrigno

Piccolini

et al. 2014

BioMed Research International

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“…falciparum 3D7HT-GFP sporozoites. Though efficient detection of parasite-specific GFP signal at the early stages of infection was hampered due to high autofluorescence in normal hepatocytes [9, 47, 48], primary cells infected with Pf 3D7HT-GFP were reproducibly detectable by flow cytometry between 72–144 hours pi (Fig 7B). It needs to be seen if flow cytometry based detection can be applied to the ex vivo detection of the liver stage parasites in the recently developed humanized mouse models of P .…”

Section: Discussionmentioning

confidence: 99%

Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro

Dumoulin

Trop

et al. 2015

PLoS ONE

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Malaria, the disease caused by Plasmodium parasites, remains a major global health burden. The liver stage of Plasmodium falciparum infection is a leading target for immunological and pharmacological interventions. Therefore, novel approaches providing specific detection and isolation of live P. falciparum exoerythrocytic forms (EEFs) are warranted. Utilizing a recently generated parasite strain expressing green fluorescent protein (GFP) we established a method which, allows for detection and isolation of developing live P. falciparum liver stages by flow cytometry. Using this technique we compared the susceptibility of five immortalized human hepatocyte cell lines and primary hepatocyte cultures from three donors to infection by P. falciparum sporozoites. Here, we show that EEFs can be detected and isolated from in vitro infected cultures of the HC-04 cell line and primary human hepatocytes. We confirmed the presence of developing parasites in sorted live human hepatocytes and characterized their morphology by fluorescence microscopy. Finally, we validated the practical applications of our approach by re-examining the importance of host ligand CD81 for hepatocyte infection by P. falciparum sporozoites in vitro and assessment of the inhibitory activity of anti-sporozoite antibodies. This methodology provides us with the tools to study both, the basic biology of the P. falciparum liver stage and the effects of host-derived factors on the development of P. falciparum EEFs.

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“…[6,11] Liver tissue represents a very enticing testing ground for fluorescence diagnostic potentials due to the numerous endogenous fluorophores involved in its several metabolic, biosynthetic, catabolic, and detoxification functions. [3] It was found out that native fluorescence characteristics of biological fluids likehuman blood plasma, [14] rat serum, [15] and human urine [11] of normal and liver diseased subjects have different fluorescence spectral characteristics. Portosystemic shunt causes decreased liver function, resulting in a number of clinical signs, which predominantly affect the central nervous system, the gastrointestinal tract, and to a smaller extent the urinary tract.…”

Section: Methodsmentioning

confidence: 99%

“…[1] FS (fluorescence spectroscopy) is a potential diagnostic technique for many disorders. [2] Successful diagnostic application of this technique could be achieved because autofluorescence alterations of human tissues [2][3][4] or body fluids [5,6] are caused by pathological process. Some studies were also performed in animal models.…”

Section: Introductionmentioning

confidence: 99%

Pilot Study of Canine Urine Analysis Using Fluorescent Fingerprint

Šteffeková

Birková

Baranová

et al. 2015

Spectroscopy Letters

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Urine contains a variety of compounds including a number of natural fluorophores. Fluorescence spectroscopy has proven to be a useful tool in analytical science. Fluorescence detection has three major advantages over other light-based investigation methods: high sensitivity, speed, and safety. Our work presents the newest approach to analysis of dog urine. Fluorescent fingerprint can very quickly reveal differences between complicated mixtures without adding any reagents. We describe autofluorescence characteristics of the urine of healthy dogs and of those with various disorders using fluorescent fingerprint. Fluorescent analysis has given good results to distinguish between pathological urine and the healthy standard in dogs. Our results suggest that this method can be used to characterize fluorescent properties of canine urine and to reveal some pathological changes in dogs.

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Human liver autofluorescence: An intrinsic tissue parameter discriminating normal and diseased conditions

Abstract: The study provides a valid premise for a development of AF-based optical biopsy techniques for a real-time discrimination of liver anatomo-pathological patterns.

Cited by 45 publications

References 39 publications

Integrated Autofluorescence Characterization of a Modified-Diet Liver Model with Accumulation of Lipids and Oxidative Stress

Integrated Autofluorescence Characterization of a Modified-Diet Liver Model with Accumulation of Lipids and Oxidative Stress

Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro

Pilot Study of Canine Urine Analysis Using Fluorescent Fingerprint

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