Human kinesin superfamily member 4 is dominantly localized in the nuclear matrix and is associated with chromosomes during mitosis

Lee, Young M.; Lee, Seungoh; Lee, Eun-Young; Shin, Hyunjin; Hahn, Hwa-Sun; Choi, Won‐Young; Kim, Wankee

doi:10.1042/0264-6021:3600549

Cited by 39 publications

(26 citation statements)

References 29 publications

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“…However, when magnified, this dot turned out to be a doublet facing each other at the apical ends of two daughter cells. Of kinesin-like proteins that are known to be required for cytokinesis, recently identified M phase phosphoprotein 1 exhibits similar pattern of localization as a doublet at midbody (Abaza et al, 2003).We did not observe this pattern of KIF4 localization in a previous study (Lee et al, 2001) where KIF4 only associates with segregating chromosomes through mitosis. The difference between the previous and to obtain the different stages of mitotic cell.…”

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confidence: 41%

“…This new antibody showed a high titer and different antigenic specificity than those antibody used in an earlier study prepared from the soluble cytosolic proteins from bacterial cell lysates (Lee et al, 2001). Western blot analysis using purified mouse and rabbit KIF4 antibodies showed a single band of 140 kDa in the HeLa cell extract (Figure 1).…”

Section: R Esults and Discussion Specificity Of K If4 Antibodiesmentioning

confidence: 99%

“…It also has been shown to be involved in the anterograde transport of vesicles containing L1, a cell adhesion molecule implicated in axonal elongation (Peretti et al, 2000). Previously we found that human KIF4 of 1,232 amino acids is a chromokinesin (Lee et al, 2001). Although it is revealed that human KIF4 interacts with BRCA2-associated factor 35 in a complex of 540 kDa (Lee and Kim, 2003), its cellular function (s) remains undefined.…”

Section: Introductionmentioning

confidence: 99%

“…After induction with IPTG, inclusion body fraction that harbors most His-tag-KIF4 fusion proteins was concentrated and used for purification of immunogen. Following the standard immunization procedures, mouse and rabbit polyclonal KIF4 antibodies were affinity-purified as described (Lee et al, 2001). Anti-a-tubulin monoclonal antibody (AB-1) was purchased from Oncogene (San Diego, CA).…”

Section: Antibodies and W Estern Blot Analysismentioning

confidence: 99%

“…We have been interested in localization of human KIF4 during the late stages of mitosis, although its other locations beside of chromosomes during mitosis were not noticed in the mitotic cells (Lee et al, 2001). With KIF4 antibodies having a different epitope specificity, we could clearly envision its localization to midzone called central spindle…”

Section: Introductionmentioning

confidence: 99%

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Kinesin superfamily protein member 4 (KIF4) is localized to midzone and midbody in dividing cells

Lee

Kim²

2004

Exp Mol Med

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Abbreviations: DAPI, 4',6-diamidino-2-phenylindole; DMEM, Dulbecco's minimum essential media; FITC, Fluorescein isothiocyanate; KIF4, kinesin superfamily protein member 4 A bstractIn association w ith m icrotubules, a variety of kinesins play im portant roles in cellular functions such as intracellular transport of organelles or vesicles, signal transduction, and cell division. In a previous study w e revealed that hum an kinesin superfam ily protein m em ber 4 (KIF4) is a chrom okinesin that binds to chrom osom es. Since localization of several kinds of kinesin at m idzone called central spindle, or m idbody that connects tw o daughter cells, or both, suggests their im plication in cell division, w e investigated K IF4 localization of during m itosis and cytokinesis in Hela cells. In addition to association w ith segregating chrom osom es through entire m itosis, it also localized to the m idzone and to m idbody at ana/telophase through cytokinesis. Especially in cells at cytokinesis, KIF4 appeared as a doublet facing each other at the apical ends of tw o daughter cells. Threedim ensional analysis of architectural relationship betw een m icrotubule bundles and KIF4 indicated that KIF4 form s a ring structure w rapping around the m icrotubule bundles. These results suggest that KIF4 is involved in cytokinesis, although direct evidence w as not provided in this study.Keywords: cell division; chromosome; kinesin; mitosis; segregation; structure IntroductionMicrotubule-dependent movement carried out by two superfamily motor proteins called cytoplasmic dynein and kinesin is fundamental to many biological processes including cell division and intracellular transport of membranous organelles in higher eukaryotic cells (Hirokawa, 1998;Rieder and Salmon, 1998;Heald and Walczak, 1999). Kinesin or kinesin-like proteins have highly conserved motor domains responsible for microtubule binding and ATP hydrolysis for movement along microtubules. In contrast, no significant sequence similarity is detected in the coiled-coil stalk and cargo-binding tail domains. The diversity of kinesin suggests that each kinesin may have a specific function.According to the recent phylogenetic analysis of kinesins (http://www.blocks.fhcrc.org/~kinesin/), kinesin superfamily member 4 (KIF4) includes 4 each vertebrate [human and murine KIF4s, chick chromokinesin, and Xenopus KLP1(Xklp1)] and invertebrate (KLP31D and KLP3A of Drosophila, and T01G1 and Y43F4B of C. elegans) kinesins. Chick chromokinesin with DNA binding activity is a nuclear protein and associates with chromosome arms (Wang and Adler, 1995), and Xklp1, which is considered to be a nuclear protein, positions mitotic chomosome and stabilizes bipolar mitotic spindle (Vernos et al., 1995). Murine KIF4 is found in both cytoplasm and nucleus, and colocalizes with membranous organelles in interphase cells and with spindle microtubules in mitotic cells (Sekine et al., 1994). It also has been shown to be involved in the anterograde transport of vesicles containing L1, a cell adhesion molecul...

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contrasting

confidence: 41%

Section: R Esults and Discussion Specificity Of K If4 Antibodiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Antibodies and W Estern Blot Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Kinesin superfamily protein member 4 (KIF4) is localized to midzone and midbody in dividing cells

Lee

Kim²

2004

Exp Mol Med

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The molecular basis for kinesin functional specificity during mitosis

Welburn

2013

Cytoskeleton

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Microtubule-based motor proteins play key roles during mitosis to assemble the bipolar spindle, define the cell division axis, and align and segregate the chromosomes. The majority of mitotic motors are members of the kinesin superfamily. Despite sharing a conserved catalytic core, each kinesin has distinct functions and localization, and is uniquely regulated in time and space. These distinct behaviors and functional specificity are generated by variations in the enzymatic domain as well as the non-conserved regions outside of the kinesin motor domain and the stalk. These flanking regions can directly modulate the properties of the kinesin motor through dimerization or self-interactions, and can associate with extrinsic factors, such as microtubule or DNA binding proteins, to provide additional functional properties. This review discusses the recently identified molecular mechanisms that explain how the control and functional specification of mitotic kinesins is achieved. © 2013 Wiley Periodicals, Inc.

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KIF4 Motor Regulates Activity-Dependent Neuronal Survival by Suppressing PARP-1 Enzymatic Activity

Midorikawa

Takei

Hirokawa

2006

Cell

105

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In brain development, apoptosis is a physiological process that controls the final numbers of neurons. Here, we report that the activity-dependent prevention of apoptosis in juvenile neurons is regulated by kinesin superfamily protein 4 (KIF4), a microtubule-based molecular motor. The C-terminal domain of KIF4 is a module that suppresses the activity of poly (ADP-ribose) polymerase-1 (PARP-1), a nuclear enzyme known to maintain cell homeostasis by repairing DNA and serving as a transcriptional regulator. When neurons are stimulated by membrane depolarization, calcium signaling mediated by CaMKII induces dissociation of KIF4 from PARP-1, resulting in upregulation of PARP-1 activity, which supports neuron survival. After dissociation from PARP-1, KIF4 enters into the cytoplasm from the nucleus and moves to the distal part of neurites in a microtubule-dependent manner. We suggested that KIF4 controls the activity-dependent survival of postmitotic neurons by regulating PARP-1 activity in brain development.

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Human kinesin superfamily member 4 is dominantly localized in the nuclear matrix and is associated with chromosomes during mitosis

Cited by 39 publications

References 29 publications

Kinesin superfamily protein member 4 (KIF4) is localized to midzone and midbody in dividing cells

Kinesin superfamily protein member 4 (KIF4) is localized to midzone and midbody in dividing cells

The molecular basis for kinesin functional specificity during mitosis

KIF4 Motor Regulates Activity-Dependent Neuronal Survival by Suppressing PARP-1 Enzymatic Activity

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